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Development of surface plasmon resonance immunosensor through metal ion affinity and mixed self-assembled monolayer.
J Microbiol Biotechnol. 2008 Oct; 18(10):1695-700.JM

Abstract

An immunosensor based on surface plasmon resonance (SPR) with enhanced performance was developed through a mixed self-assembled monolayer. A mixture of 16- mercaptohexadecanic acid (16-MHA) and 1-undecanethiol with various molar ratios was self-assembled on gold (Au) surface and the carboxylic acid groups of 16-MHA were then coordinated to Zn ions by exposing the substrate to an ethanolic solution of Zn(NO(3))(2)d6H2O. The antibody was immobilized on the SPR surface by exposing the functionalized substrate to the desired solution of antibody in phosphatebuffered saline (PBS) molecules. The film formation in series was confirmed by SPR and atomic force microscopy (AFM). The functionalized surface was applied to develop an SPR immunosensor for detecting human serum albumin (HSA) and the estimated detection limit (DL) was 4.27 nM. The limit value concentration can be well measured between ill and healthy conditions.

Authors+Show Affiliations

Lee S
Department of Chemical and Biomolecular Engineering, Sogang University, Seoul 121-741, Korea.
Sim SJ
No affiliation info available
Park C
No affiliation info available
Gu MB
No affiliation info available
Hwang UY
No affiliation info available
Yi J
No affiliation info available
Oh BK
No affiliation info available
Lee J
No affiliation info available

MeSH

AntibodiesGoldHumansImmunoassayIonsPalmitic AcidsSerum AlbuminSurface Plasmon ResonanceSurface Properties

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

18955822

Citation

Lee, Sira, et al. "Development of Surface Plasmon Resonance Immunosensor Through Metal Ion Affinity and Mixed Self-assembled Monolayer." Journal of Microbiology and Biotechnology, vol. 18, no. 10, 2008, pp. 1695-700.
Lee S, Sim SJ, Park C, et al. Development of surface plasmon resonance immunosensor through metal ion affinity and mixed self-assembled monolayer. J Microbiol Biotechnol. 2008;18(10):1695-700.
Lee, S., Sim, S. J., Park, C., Gu, M. B., Hwang, U. Y., Yi, J., Oh, B. K., & Lee, J. (2008). Development of surface plasmon resonance immunosensor through metal ion affinity and mixed self-assembled monolayer. Journal of Microbiology and Biotechnology, 18(10), 1695-700.
Lee S, et al. Development of Surface Plasmon Resonance Immunosensor Through Metal Ion Affinity and Mixed Self-assembled Monolayer. J Microbiol Biotechnol. 2008;18(10):1695-700. PubMed PMID: 18955822.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of surface plasmon resonance immunosensor through metal ion affinity and mixed self-assembled monolayer. AU - Lee,Sira, AU - Sim,Sang-Jun, AU - Park,Chulhwan, AU - Gu,Man Bock, AU - Hwang,Un Yeon, AU - Yi,Jongheop, AU - Oh,Byung-Keun, AU - Lee,Jinwon, PY - 2008/10/29/pubmed PY - 2009/6/9/medline PY - 2008/10/29/entrez SP - 1695 EP - 700 JF - Journal of microbiology and biotechnology JO - J Microbiol Biotechnol VL - 18 IS - 10 N2 - An immunosensor based on surface plasmon resonance (SPR) with enhanced performance was developed through a mixed self-assembled monolayer. A mixture of 16- mercaptohexadecanic acid (16-MHA) and 1-undecanethiol with various molar ratios was self-assembled on gold (Au) surface and the carboxylic acid groups of 16-MHA were then coordinated to Zn ions by exposing the substrate to an ethanolic solution of Zn(NO(3))(2)d6H2O. The antibody was immobilized on the SPR surface by exposing the functionalized substrate to the desired solution of antibody in phosphatebuffered saline (PBS) molecules. The film formation in series was confirmed by SPR and atomic force microscopy (AFM). The functionalized surface was applied to develop an SPR immunosensor for detecting human serum albumin (HSA) and the estimated detection limit (DL) was 4.27 nM. The limit value concentration can be well measured between ill and healthy conditions. SN - 1017-7825 UR - https://www.unboundmedicine.com/medline/citation/18955822/Development_of_surface_plasmon_resonance_immunosensor_through_metal_ion_affinity_and_mixed_self_assembled_monolayer_ DB - PRIME DP - Unbound Medicine ER -