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THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association.
Cell. 2008 Oct 17; 135(2):308-21.Cell

Abstract

During transcription, proteins assemble sequentially with nascent RNA to generate a messenger ribonucleoprotein particle (mRNP). The THO complex and its associated Sub2p helicase are functionally implicated in both transcription and mRNP biogenesis but their precise function remains elusive. We show here that THO/Sub2p mutation leads to the accumulation of a stalled intermediate in mRNP biogenesis that contains nuclear pore components and polyadenylation factors in association with chromatin. Microarray analyses of genomic loci that are aberrantly docked to the nuclear pore in mutants allowed the identification of approximately 400 novel validated target genes that require THO /Sub2p for efficient expression. Our data strongly suggests that the THO complex/Sub2p function is required to coordinate events leading to the acquisition of export competence at a step that follows commitment to 3'-processing.

Authors+Show Affiliations

LEA Laboratory of Nuclear RNA metabolism, Centre de Génétique Moléculaire, Centre National de la Recherche Scientifique - UPR2167, 1, av de la Terrasse, 91190, Gif sur Yvette, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18957205

Citation

Rougemaille, Mathieu, et al. "THO/Sub2p Functions to Coordinate 3'-end Processing With Gene-nuclear Pore Association." Cell, vol. 135, no. 2, 2008, pp. 308-21.
Rougemaille M, Dieppois G, Kisseleva-Romanova E, et al. THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association. Cell. 2008;135(2):308-21.
Rougemaille, M., Dieppois, G., Kisseleva-Romanova, E., Gudipati, R. K., Lemoine, S., Blugeon, C., Boulay, J., Jensen, T. H., Stutz, F., Devaux, F., & Libri, D. (2008). THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association. Cell, 135(2), 308-21. https://doi.org/10.1016/j.cell.2008.08.005
Rougemaille M, et al. THO/Sub2p Functions to Coordinate 3'-end Processing With Gene-nuclear Pore Association. Cell. 2008 Oct 17;135(2):308-21. PubMed PMID: 18957205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association. AU - Rougemaille,Mathieu, AU - Dieppois,Guennaelle, AU - Kisseleva-Romanova,Elena, AU - Gudipati,Rajani Kanth, AU - Lemoine,Sophie, AU - Blugeon,Corinne, AU - Boulay,Jocelyne, AU - Jensen,Torben Heick, AU - Stutz,Françoise, AU - Devaux,Frédéric, AU - Libri,Domenico, PY - 2007/05/27/received PY - 2008/03/26/revised PY - 2008/08/01/accepted PY - 2008/10/30/pubmed PY - 2008/11/15/medline PY - 2008/10/30/entrez SP - 308 EP - 21 JF - Cell JO - Cell VL - 135 IS - 2 N2 - During transcription, proteins assemble sequentially with nascent RNA to generate a messenger ribonucleoprotein particle (mRNP). The THO complex and its associated Sub2p helicase are functionally implicated in both transcription and mRNP biogenesis but their precise function remains elusive. We show here that THO/Sub2p mutation leads to the accumulation of a stalled intermediate in mRNP biogenesis that contains nuclear pore components and polyadenylation factors in association with chromatin. Microarray analyses of genomic loci that are aberrantly docked to the nuclear pore in mutants allowed the identification of approximately 400 novel validated target genes that require THO /Sub2p for efficient expression. Our data strongly suggests that the THO complex/Sub2p function is required to coordinate events leading to the acquisition of export competence at a step that follows commitment to 3'-processing. SN - 1097-4172 UR - https://www.unboundmedicine.com/medline/citation/18957205/THO/Sub2p_functions_to_coordinate_3'_end_processing_with_gene_nuclear_pore_association_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0092-8674(08)01006-4 DB - PRIME DP - Unbound Medicine ER -