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Impact of cranberry on Escherichia coli cellular surface characteristics.
Biochem Biophys Res Commun 2008; 377(3):992-4BB

Abstract

The anti-adhesive effects of cranberry have been attributed to both interactions of its components with the surface of bacterial cells and to inhibition of p-fimbriae expression. Previous reports also suggested that the presence of cranberry juice changed the Gram stain characteristics of Escherichia coli. Here, we show that the morphology of E. coli is changed when grown in the presence of juice or extract from Vaccinium macrocarpon (cranberry). Gene expression analysis indicates the down regulation of flagellar basal body rod and motor proteins. Consistent with this finding and previous reports, the SEM images indicate a decrease in the visible p-fimbriae. The iodine used in Gram-staining protocols was found to interact differently with the bacterial membrane when cells were cultured in spiked media. Slight alterations in the Gram stain protocol demonstrated that culturing in the presence of cranberry juice does not change the Gram stain characteristics contradicting other reports.

Authors+Show Affiliations

Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, 4555 Overlook Ave SW, Code 6900, Washington, DC 20375, USA. brandy.white@nrl.navy.milNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

18957286

Citation

Johnson, Brandy J., et al. "Impact of Cranberry On Escherichia Coli Cellular Surface Characteristics." Biochemical and Biophysical Research Communications, vol. 377, no. 3, 2008, pp. 992-4.
Johnson BJ, Lin B, Dinderman MA, et al. Impact of cranberry on Escherichia coli cellular surface characteristics. Biochem Biophys Res Commun. 2008;377(3):992-4.
Johnson, B. J., Lin, B., Dinderman, M. A., Rubin, R. A., Malanoski, A. P., & Ligler, F. S. (2008). Impact of cranberry on Escherichia coli cellular surface characteristics. Biochemical and Biophysical Research Communications, 377(3), pp. 992-4. doi:10.1016/j.bbrc.2008.10.098.
Johnson BJ, et al. Impact of Cranberry On Escherichia Coli Cellular Surface Characteristics. Biochem Biophys Res Commun. 2008 Dec 19;377(3):992-4. PubMed PMID: 18957286.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Impact of cranberry on Escherichia coli cellular surface characteristics. AU - Johnson,Brandy J, AU - Lin,Baochuan, AU - Dinderman,Michael A, AU - Rubin,Robert A, AU - Malanoski,Anthony P, AU - Ligler,Frances S, Y1 - 2008/10/26/ PY - 2008/10/01/received PY - 2008/10/20/accepted PY - 2008/10/30/pubmed PY - 2008/12/17/medline PY - 2008/10/30/entrez SP - 992 EP - 4 JF - Biochemical and biophysical research communications JO - Biochem. Biophys. Res. Commun. VL - 377 IS - 3 N2 - The anti-adhesive effects of cranberry have been attributed to both interactions of its components with the surface of bacterial cells and to inhibition of p-fimbriae expression. Previous reports also suggested that the presence of cranberry juice changed the Gram stain characteristics of Escherichia coli. Here, we show that the morphology of E. coli is changed when grown in the presence of juice or extract from Vaccinium macrocarpon (cranberry). Gene expression analysis indicates the down regulation of flagellar basal body rod and motor proteins. Consistent with this finding and previous reports, the SEM images indicate a decrease in the visible p-fimbriae. The iodine used in Gram-staining protocols was found to interact differently with the bacterial membrane when cells were cultured in spiked media. Slight alterations in the Gram stain protocol demonstrated that culturing in the presence of cranberry juice does not change the Gram stain characteristics contradicting other reports. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/18957286/Impact_of_cranberry_on_Escherichia_coli_cellular_surface_characteristics_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(08)02091-3 DB - PRIME DP - Unbound Medicine ER -