Tags

Type your tag names separated by a space and hit enter

Intratracheal versus intravenous liposomal delivery of siRNA, antisense oligonucleotides and anticancer drug.
Pharm Res. 2009 Feb; 26(2):382-94.PR

Abstract

PURPOSE

To compare systemic intravenous and local intratracheal delivery of doxorubicin (DOX), antisense oligonucleotides (ASO) and small interfering RNA (siRNA).

METHODS

"Neutral" and cationic liposomes were used to deliver DOX, ASO, and siRNA. Liposomes were characterized by dynamic light scattering, zeta-potential, and atomic force microscopy. Cellular internalization of DOX, ASO and siRNA was studied by confocal microscopy on human lung carcinoma cells. In vivo experiments were carried out on nude mice with an orthotopic model of human lung cancer.

RESULTS

Liposomes provided for an efficient intracellular delivery of DOX, ASO, and siRNA in vitro. Intratracheal delivery of both types of liposomes in vivo led to higher peak concentrations and much longer retention of liposomes, DOX, ASO and siRNA in the lungs when compared with systemic administration. It was found that local intratracheal treatment of lung cancer with liposomal DOX was more efficient when compared with free and liposomal DOX delivered intravenously.

CONCLUSIONS

The present study outlined the clear advantages of local intratracheal delivery of liposomal drugs for the treatment of lung cancer when compared with systemic administration of the same drug.

Authors+Show Affiliations

Department of Pharmaceutics, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, New Jersey 08854-8020, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18958402

Citation

Garbuzenko, Olga B., et al. "Intratracheal Versus Intravenous Liposomal Delivery of siRNA, Antisense Oligonucleotides and Anticancer Drug." Pharmaceutical Research, vol. 26, no. 2, 2009, pp. 382-94.
Garbuzenko OB, Saad M, Betigeri S, et al. Intratracheal versus intravenous liposomal delivery of siRNA, antisense oligonucleotides and anticancer drug. Pharm Res. 2009;26(2):382-94.
Garbuzenko, O. B., Saad, M., Betigeri, S., Zhang, M., Vetcher, A. A., Soldatenkov, V. A., Reimer, D. C., Pozharov, V. P., & Minko, T. (2009). Intratracheal versus intravenous liposomal delivery of siRNA, antisense oligonucleotides and anticancer drug. Pharmaceutical Research, 26(2), 382-94. https://doi.org/10.1007/s11095-008-9755-4
Garbuzenko OB, et al. Intratracheal Versus Intravenous Liposomal Delivery of siRNA, Antisense Oligonucleotides and Anticancer Drug. Pharm Res. 2009;26(2):382-94. PubMed PMID: 18958402.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intratracheal versus intravenous liposomal delivery of siRNA, antisense oligonucleotides and anticancer drug. AU - Garbuzenko,Olga B, AU - Saad,Maha, AU - Betigeri,Seema, AU - Zhang,Min, AU - Vetcher,Alexandre A, AU - Soldatenkov,Viatcheslav A, AU - Reimer,David C, AU - Pozharov,Vitaly P, AU - Minko,Tamara, Y1 - 2008/10/29/ PY - 2008/06/01/received PY - 2008/10/10/accepted PY - 2008/10/30/pubmed PY - 2009/3/24/medline PY - 2008/10/30/entrez SP - 382 EP - 94 JF - Pharmaceutical research JO - Pharm Res VL - 26 IS - 2 N2 - PURPOSE: To compare systemic intravenous and local intratracheal delivery of doxorubicin (DOX), antisense oligonucleotides (ASO) and small interfering RNA (siRNA). METHODS: "Neutral" and cationic liposomes were used to deliver DOX, ASO, and siRNA. Liposomes were characterized by dynamic light scattering, zeta-potential, and atomic force microscopy. Cellular internalization of DOX, ASO and siRNA was studied by confocal microscopy on human lung carcinoma cells. In vivo experiments were carried out on nude mice with an orthotopic model of human lung cancer. RESULTS: Liposomes provided for an efficient intracellular delivery of DOX, ASO, and siRNA in vitro. Intratracheal delivery of both types of liposomes in vivo led to higher peak concentrations and much longer retention of liposomes, DOX, ASO and siRNA in the lungs when compared with systemic administration. It was found that local intratracheal treatment of lung cancer with liposomal DOX was more efficient when compared with free and liposomal DOX delivered intravenously. CONCLUSIONS: The present study outlined the clear advantages of local intratracheal delivery of liposomal drugs for the treatment of lung cancer when compared with systemic administration of the same drug. SN - 0724-8741 UR - https://www.unboundmedicine.com/medline/citation/18958402/Intratracheal_versus_intravenous_liposomal_delivery_of_siRNA_antisense_oligonucleotides_and_anticancer_drug_ DB - PRIME DP - Unbound Medicine ER -