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The mechanism of the late preconditioning effect of 3-nitropropionic acid.
Arch Pharm Res. 2008 Oct; 31(10):1257-63.AP

Abstract

The aim of the present study was to investigate the mechanism of effect of 3-nitropropionic acid-(3-NP) induced late preconditioning in rat heart. For this purpose 20-30 min before 3-NP (20 mg/kg, i.p.) injection, the rats were treated intraperitoneally with 5-hydroxydecanoate (40 mg/kg, 5-HD, mitochondrial K(ATP)-channel blocker), L-NAME (100 mg/kg, NOS inhibitor), N-2-mercaptopropionylglycine (100 mg/kg, MPG, free radical scavenger), or superoxide dismutase+catalase (10000+10000 IU/kg, SOD+CAT). Control rats received saline only without 3-NP pretreatment. After two days, hearts were isolated and perfused at a constant pressure in a Langendorff apparatus. 15-min global ischemia followed by 30-min reperfusion was applied to all hearts. Pretreatment of 3-NP significantly reduced infarct size, creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH) levels, and incidence of ventricular tachycardia (VT) compared with the control group receiving saline only. 5-HD, L-NAME, MPG, or SOD+CAT treatment statistically reversed 3-NP-induced reduction in infarct size. Although CK-MB, LDH levels, and incidence of VT were also reduced by L-NAME, MPG, or SOD+CAT treatment, only 5-HD significantly inhibited beneficial effects of 3-NP on all of the parameters above. These results showed that mito-K(ATP) channels play a pivotal role in late preconditioning effect of 3-NP in the isolated rat heart. However, other mediators such as reactive oxygen species and NO may be, at least in part, involved in mechanisms of this effect.

Authors+Show Affiliations

Gazi University, Faculty of Pharmacy, Department of Pharmacology, Etiler, Ankara, Turkey. bilgenbasgut@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18958415

Citation

Basgut, Bilgen, et al. "The Mechanism of the Late Preconditioning Effect of 3-nitropropionic Acid." Archives of Pharmacal Research, vol. 31, no. 10, 2008, pp. 1257-63.
Basgut B, Aypar E, Basgut E, et al. The mechanism of the late preconditioning effect of 3-nitropropionic acid. Arch Pharm Res. 2008;31(10):1257-63.
Basgut, B., Aypar, E., Basgut, E., Akin, K. O., Kilic, N., & Cakici, I. (2008). The mechanism of the late preconditioning effect of 3-nitropropionic acid. Archives of Pharmacal Research, 31(10), 1257-63. https://doi.org/10.1007/s12272-001-2104-3
Basgut B, et al. The Mechanism of the Late Preconditioning Effect of 3-nitropropionic Acid. Arch Pharm Res. 2008;31(10):1257-63. PubMed PMID: 18958415.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The mechanism of the late preconditioning effect of 3-nitropropionic acid. AU - Basgut,Bilgen, AU - Aypar,Eda, AU - Basgut,Ertug, AU - Akin,K Okhan, AU - Kilic,Nedret, AU - Cakici,Iclal, Y1 - 2008/10/29/ PY - 2008/01/09/received PY - 2008/10/06/accepted PY - 2008/10/06/revised PY - 2008/10/30/pubmed PY - 2008/12/17/medline PY - 2008/10/30/entrez SP - 1257 EP - 63 JF - Archives of pharmacal research JO - Arch Pharm Res VL - 31 IS - 10 N2 - The aim of the present study was to investigate the mechanism of effect of 3-nitropropionic acid-(3-NP) induced late preconditioning in rat heart. For this purpose 20-30 min before 3-NP (20 mg/kg, i.p.) injection, the rats were treated intraperitoneally with 5-hydroxydecanoate (40 mg/kg, 5-HD, mitochondrial K(ATP)-channel blocker), L-NAME (100 mg/kg, NOS inhibitor), N-2-mercaptopropionylglycine (100 mg/kg, MPG, free radical scavenger), or superoxide dismutase+catalase (10000+10000 IU/kg, SOD+CAT). Control rats received saline only without 3-NP pretreatment. After two days, hearts were isolated and perfused at a constant pressure in a Langendorff apparatus. 15-min global ischemia followed by 30-min reperfusion was applied to all hearts. Pretreatment of 3-NP significantly reduced infarct size, creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH) levels, and incidence of ventricular tachycardia (VT) compared with the control group receiving saline only. 5-HD, L-NAME, MPG, or SOD+CAT treatment statistically reversed 3-NP-induced reduction in infarct size. Although CK-MB, LDH levels, and incidence of VT were also reduced by L-NAME, MPG, or SOD+CAT treatment, only 5-HD significantly inhibited beneficial effects of 3-NP on all of the parameters above. These results showed that mito-K(ATP) channels play a pivotal role in late preconditioning effect of 3-NP in the isolated rat heart. However, other mediators such as reactive oxygen species and NO may be, at least in part, involved in mechanisms of this effect. SN - 0253-6269 UR - https://www.unboundmedicine.com/medline/citation/18958415/The_mechanism_of_the_late_preconditioning_effect_of_3_nitropropionic_acid_ L2 - https://dx.doi.org/10.1007/s12272-001-2104-3 DB - PRIME DP - Unbound Medicine ER -