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Flavonoids inhibit histamine release and expression of proinflammatory cytokines in mast cells.
Arch Pharm Res. 2008 Oct; 31(10):1303-11.AP

Abstract

Mast cells participate in allergy and inflammation by secreting inflammatory mediators such as histamine and proinflammatory cytokines. Flavonoids are naturally occurring molecules with antioxidant, cytoprotective, and antiinflammatory actions. However, effect of flavonoids on the release of histamine and proinflammatory mediator, and their comparative mechanism of action in mast cells were not well defined. Here, we compared the effect of six flavonoids (astragalin, fisetin, kaempferol, myricetin, quercetin, and rutin) on the mast cell-mediated allergic inflammation. Fisetin, kaempferol, myricetin, quercetin, and rutin inhibited IgE or phorbol-12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-mediated histamine release in RBL-2H3 cells. These five flavonoids also inhibited elevation of intracellular calcium. Gene expressions and secretion of proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta, IL-6, and IL-8 were assessed in PMACI-stimulated human mast cells (HMC-1). Fisetin, quercetin, and rutin decreased gene expression and production of all the proinflammatory cytokines after PMACI stimulation. Myricetin attenuated TNF-alpha and IL-6 but not IL-1beta and IL-8. Fisetin, myricetin, and rutin suppressed activation of NF-kappaB indicated by inhibition of nuclear translocation of NF-kappaB, NF-kappaB/DNA binding, and NF-kappaB-dependent gene reporter assay. The pharmacological actions of these flavonoids suggest their potential activity for treatment of allergic inflammatory diseases through the down-regulation of mast cell activation.

Authors+Show Affiliations

CMRI, Department of Pharmacology, Kyungpook National University, Daegu 700-422, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18958421

Citation

Park, Hyo-Hyun, et al. "Flavonoids Inhibit Histamine Release and Expression of Proinflammatory Cytokines in Mast Cells." Archives of Pharmacal Research, vol. 31, no. 10, 2008, pp. 1303-11.
Park HH, Lee S, Son HY, et al. Flavonoids inhibit histamine release and expression of proinflammatory cytokines in mast cells. Arch Pharm Res. 2008;31(10):1303-11.
Park, H. H., Lee, S., Son, H. Y., Park, S. B., Kim, M. S., Choi, E. J., Singh, T. S., Ha, J. H., Lee, M. G., Kim, J. E., Hyun, M. C., Kwon, T. K., Kim, Y. H., & Kim, S. H. (2008). Flavonoids inhibit histamine release and expression of proinflammatory cytokines in mast cells. Archives of Pharmacal Research, 31(10), 1303-11. https://doi.org/10.1007/s12272-001-2110-5
Park HH, et al. Flavonoids Inhibit Histamine Release and Expression of Proinflammatory Cytokines in Mast Cells. Arch Pharm Res. 2008;31(10):1303-11. PubMed PMID: 18958421.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Flavonoids inhibit histamine release and expression of proinflammatory cytokines in mast cells. AU - Park,Hyo-Hyun, AU - Lee,Soyoung, AU - Son,Hee-Young, AU - Park,Seung-Bin, AU - Kim,Mi-Sun, AU - Choi,Eun-Ju, AU - Singh,Thoudam S K, AU - Ha,Jeoung-Hee, AU - Lee,Maan-Gee, AU - Kim,Jung-Eun, AU - Hyun,Myung Chul, AU - Kwon,Taeg Kyu, AU - Kim,Yeo Hyang, AU - Kim,Sang-Hyun, Y1 - 2008/10/29/ PY - 2008/06/20/received PY - 2008/10/06/accepted PY - 2008/10/06/revised PY - 2008/10/30/pubmed PY - 2008/12/17/medline PY - 2008/10/30/entrez SP - 1303 EP - 11 JF - Archives of pharmacal research JO - Arch Pharm Res VL - 31 IS - 10 N2 - Mast cells participate in allergy and inflammation by secreting inflammatory mediators such as histamine and proinflammatory cytokines. Flavonoids are naturally occurring molecules with antioxidant, cytoprotective, and antiinflammatory actions. However, effect of flavonoids on the release of histamine and proinflammatory mediator, and their comparative mechanism of action in mast cells were not well defined. Here, we compared the effect of six flavonoids (astragalin, fisetin, kaempferol, myricetin, quercetin, and rutin) on the mast cell-mediated allergic inflammation. Fisetin, kaempferol, myricetin, quercetin, and rutin inhibited IgE or phorbol-12-myristate 13-acetate and calcium ionophore A23187 (PMACI)-mediated histamine release in RBL-2H3 cells. These five flavonoids also inhibited elevation of intracellular calcium. Gene expressions and secretion of proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta, IL-6, and IL-8 were assessed in PMACI-stimulated human mast cells (HMC-1). Fisetin, quercetin, and rutin decreased gene expression and production of all the proinflammatory cytokines after PMACI stimulation. Myricetin attenuated TNF-alpha and IL-6 but not IL-1beta and IL-8. Fisetin, myricetin, and rutin suppressed activation of NF-kappaB indicated by inhibition of nuclear translocation of NF-kappaB, NF-kappaB/DNA binding, and NF-kappaB-dependent gene reporter assay. The pharmacological actions of these flavonoids suggest their potential activity for treatment of allergic inflammatory diseases through the down-regulation of mast cell activation. SN - 0253-6269 UR - https://www.unboundmedicine.com/medline/citation/18958421/Flavonoids_inhibit_histamine_release_and_expression_of_proinflammatory_cytokines_in_mast_cells_ L2 - https://dx.doi.org/10.1007/s12272-001-2110-5 DB - PRIME DP - Unbound Medicine ER -