TY - JOUR T1 - Membrane preconcentration-capillary electrophoresis tandem mass spectrometry (mPC-CE-MS/MS) in the sequence analysis of biologically derived peptides. AU - Naylor,S, AU - Tomlinson,A J, PY - 1997/02/05/received PY - 1997/06/02/revised PY - 1997/06/04/accepted PY - 2008/10/31/pubmed PY - 2008/10/31/medline PY - 2008/10/31/entrez SP - 603 EP - 12 JF - Talanta JO - Talanta VL - 45 IS - 4 N2 - We demonstrate the use of membrane preconcentration capillary electrophoresis tandem mass spectrometry (mPC-CE-MS/MS) for sequencing peptides at the sub-100 femtomole level. In particular by loading the mPC-CE cartridge off-line with a pressurized bomb apparatus, 100 mul solutions can be loaded in <5 min. Furthermore, mPC-CE-MS in conjunction with on-line transient isotachophoresis carried out in 25 mum i.d. capillaries results in enhanced resolution and theoretical plate values as compared to convention 50-75 mum i.d. capillaries. We show that this is a powerful new approach in the sequencing of biologically derived compounds from complex mixtures such as MHC class I peptides. SN - 0039-9140 UR - https://www.unboundmedicine.com/medline/citation/18967042/Membrane_preconcentration_capillary_electrophoresis_tandem_mass_spectrometry__mPC_CE_MS/MS__in_the_sequence_analysis_of_biologically_derived_peptides_ DB - PRIME DP - Unbound Medicine ER -