TY - JOUR T1 - Simultaneous micellar electrokinetic chromatography and liquid chromatography of adriblastina and tarabine PFS Their application to some biological fluids. AU - El-Hady,Deia Abd, AU - El-Maali,Nagwa Abo, AU - Gotti,Roberto, AU - Andrisano,Vincenza, Y1 - 2004/12/24/ PY - 2004/05/06/received PY - 2004/09/08/revised PY - 2004/11/23/accepted PY - 2008/10/31/pubmed PY - 2008/10/31/medline PY - 2008/10/31/entrez SP - 253 EP - 60 JF - Talanta JO - Talanta VL - 66 IS - 1 N2 - The current work presents analytical procedures for simultaneous determination of tarabine PFS and adriblastina by micellar electrokinetic chromatography (MEKC) and liquid chromatography (LC). For MEKC analysis, separations and identifications were accomplished using uncoated fused-silica capillary with hydrodynamic injections in the presence of 50mM borate/phophate pH 8.7 and 100mM SDS. The migration times of tarabine PFS and adriblastina were found to be 2.70 and 6.40min, respectively. Calibration curves were established for 10-300ng/mL (r=0.998) tarabine PFS and for 8-120microg/mL (r=0.999) adriblastina. For LC analysis, separations were performed on teicoplanin stationary phase with reversed mobile phase containing methanol:buffer pH 4.05 (20:80%, v/v) at 285nm. The retention times of tarabine PFS and adriblastina were 5.18 and 7.20min, respectively. Calibration curves were established for 3-90microg/mL (r=0.998) tarabine PFS and for 10-120microg/mL (r=0.999) adriblastina. Both MEKC and LC methods were applied for the simultaneous determination of analytes in urine samples. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/18969990/Simultaneous_micellar_electrokinetic_chromatography_and_liquid_chromatography_of_adriblastina_and_tarabine_PFS_Their_application_to_some_biological_fluids_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(04)00682-4 DB - PRIME DP - Unbound Medicine ER -