TY - JOUR T1 - Determination of amphetamine and methamphetamine in urine by solid phase extraction and ion-pair liquid chromatography-electrospray-tandem mass spectrometry. AU - Fuh,Ming-Ren, AU - Wu,Ti-Yu, AU - Lin,Tzuen-Yeuan, Y1 - 2005/08/02/ PY - 2005/04/18/received PY - 2005/06/29/revised PY - 2005/06/29/accepted PY - 2008/10/31/pubmed PY - 2008/10/31/medline PY - 2008/10/31/entrez SP - 987 EP - 91 JF - Talanta JO - Talanta VL - 68 IS - 3 N2 - A method using a solid phase extraction (SPE) and ion-pair liquid chromatography-electrospray-tandem mass spectrometry (LC-ES-MS/MS) was developed for determination of amphetamine (Amp) and methamphetamine (mAmp) in urine samples. A reversed phase C(18) column was utilized for LC separation and MS/MS was used for detection. Trifluoroacetic acid was added to the mobile phase as an ion-pairing reagent. MS(2) was employed for quantitative determination. In addition, d(8)-amphetamine and d(8)-methamphetamine were used as internal standards. An Oasis HLB SPE cartridge, which has hydrophilic and lipophilic functions, was utilized for sample pre-treatment. Recoveries ranging from 97.3 to 102.1% were measured. Good linear ranges, 5-500 ng/ml, for Amp and mAmp were determined. The detection limit of each analytical compound, based on a signal-to-noise ratio of 3, was approximately 1 ng/ml. The applicability of this newly developed method was examined by analyzing several urine samples from drug users. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/18970421/Determination_of_amphetamine_and_methamphetamine_in_urine_by_solid_phase_extraction_and_ion_pair_liquid_chromatography_electrospray_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(05)00410-8 DB - PRIME DP - Unbound Medicine ER -