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Determination of amphetamine and methamphetamine in urine by solid phase extraction and ion-pair liquid chromatography-electrospray-tandem mass spectrometry.
Talanta. 2006 Jan 15; 68(3):987-91.T

Abstract

A method using a solid phase extraction (SPE) and ion-pair liquid chromatography-electrospray-tandem mass spectrometry (LC-ES-MS/MS) was developed for determination of amphetamine (Amp) and methamphetamine (mAmp) in urine samples. A reversed phase C(18) column was utilized for LC separation and MS/MS was used for detection. Trifluoroacetic acid was added to the mobile phase as an ion-pairing reagent. MS(2) was employed for quantitative determination. In addition, d(8)-amphetamine and d(8)-methamphetamine were used as internal standards. An Oasis HLB SPE cartridge, which has hydrophilic and lipophilic functions, was utilized for sample pre-treatment. Recoveries ranging from 97.3 to 102.1% were measured. Good linear ranges, 5-500 ng/ml, for Amp and mAmp were determined. The detection limit of each analytical compound, based on a signal-to-noise ratio of 3, was approximately 1 ng/ml. The applicability of this newly developed method was examined by analyzing several urine samples from drug users.

Authors+Show Affiliations

Department of Chemistry, Soochow University, Taipei, Taiwan. msfuh@mail.scu.edu.twNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18970421

Citation

Fuh, Ming-Ren, et al. "Determination of Amphetamine and Methamphetamine in Urine By Solid Phase Extraction and Ion-pair Liquid Chromatography-electrospray-tandem Mass Spectrometry." Talanta, vol. 68, no. 3, 2006, pp. 987-91.
Fuh MR, Wu TY, Lin TY. Determination of amphetamine and methamphetamine in urine by solid phase extraction and ion-pair liquid chromatography-electrospray-tandem mass spectrometry. Talanta. 2006;68(3):987-91.
Fuh, M. R., Wu, T. Y., & Lin, T. Y. (2006). Determination of amphetamine and methamphetamine in urine by solid phase extraction and ion-pair liquid chromatography-electrospray-tandem mass spectrometry. Talanta, 68(3), 987-91. https://doi.org/10.1016/j.talanta.2005.06.057
Fuh MR, Wu TY, Lin TY. Determination of Amphetamine and Methamphetamine in Urine By Solid Phase Extraction and Ion-pair Liquid Chromatography-electrospray-tandem Mass Spectrometry. Talanta. 2006 Jan 15;68(3):987-91. PubMed PMID: 18970421.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of amphetamine and methamphetamine in urine by solid phase extraction and ion-pair liquid chromatography-electrospray-tandem mass spectrometry. AU - Fuh,Ming-Ren, AU - Wu,Ti-Yu, AU - Lin,Tzuen-Yeuan, Y1 - 2005/08/02/ PY - 2005/04/18/received PY - 2005/06/29/revised PY - 2005/06/29/accepted PY - 2008/10/31/pubmed PY - 2008/10/31/medline PY - 2008/10/31/entrez SP - 987 EP - 91 JF - Talanta JO - Talanta VL - 68 IS - 3 N2 - A method using a solid phase extraction (SPE) and ion-pair liquid chromatography-electrospray-tandem mass spectrometry (LC-ES-MS/MS) was developed for determination of amphetamine (Amp) and methamphetamine (mAmp) in urine samples. A reversed phase C(18) column was utilized for LC separation and MS/MS was used for detection. Trifluoroacetic acid was added to the mobile phase as an ion-pairing reagent. MS(2) was employed for quantitative determination. In addition, d(8)-amphetamine and d(8)-methamphetamine were used as internal standards. An Oasis HLB SPE cartridge, which has hydrophilic and lipophilic functions, was utilized for sample pre-treatment. Recoveries ranging from 97.3 to 102.1% were measured. Good linear ranges, 5-500 ng/ml, for Amp and mAmp were determined. The detection limit of each analytical compound, based on a signal-to-noise ratio of 3, was approximately 1 ng/ml. The applicability of this newly developed method was examined by analyzing several urine samples from drug users. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/18970421/Determination_of_amphetamine_and_methamphetamine_in_urine_by_solid_phase_extraction_and_ion_pair_liquid_chromatography_electrospray_tandem_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(05)00410-8 DB - PRIME DP - Unbound Medicine ER -
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