TY - JOUR T1 - Simultaneous detection of Escherichia coli O157:H7, Salmonella spp. and Listeria monocytogenes with an array-based immunosorbent assay using universal protein G-liposomal nanovesicles. AU - Chen,Chien-Sheng, AU - Durst,Richard A, Y1 - 2005/10/27/ PY - 2005/07/08/received PY - 2005/09/27/revised PY - 2005/09/27/accepted PY - 2008/10/31/pubmed PY - 2008/10/31/medline PY - 2008/10/31/entrez SP - 232 EP - 8 JF - Talanta JO - Talanta VL - 69 IS - 1 N2 - A novel universal reagent for immunoassays, protein G-liposomal nanovesicles has been developed and successfully used in an immunomagnetic bead sandwich assay for the detection of Escherichia coli O157:H7 [C.-S. Chen, A.J. Baeumner, R.A. Durst, Talanta 67 (2005) 205]. To demonstrate the universal capability of protein G-liposomal nanovesicles, this reagent was used to develop an array-based immunosorbent assay for the simultaneous detection of E. coli O157:H7, Salmonella, and Listeria monocytogenes. Both direct and competitive immunoassay formats were used to demonstrate the feasibility of detecting multiple analytes in a single test by using universal protein G-liposomal nanovesicles. Both pure and mixed cultures were examined in the direct immunoassay format. Results indicate that the limits of detection (LODs) of the direct assay for E. coli O157:H7, Salmonella enterica serovar Typhimurium and L. monocytogenes in pure cultures were approximately 100, 500 and 1.5 x 10(4)CFU/ml, respectively. In mixed cultures, the LODs were approximately 3.1 x 10(3), 7.8 x 10(4), and 7.9 x 10(5)CFU/ml. In the competitive assay format, the LODs for E. coli O157:H7, S. enterica serovar Typhimurium, and L. monocytogenes were approximately 1.5 x 10(4), 5 x 10(4), and 1.2 x 10(5)CFU/ml for the pure cultures. These results showed that protein G-liposomal nanovesicles can be successfully used in a simultaneous immunoassay for several food-borne pathogens, thereby demonstrating that they are effective universal reagents for use in immunoassays. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/18970559/Simultaneous_detection_of_Escherichia_coli_O157:H7_Salmonella_spp__and_Listeria_monocytogenes_with_an_array_based_immunosorbent_assay_using_universal_protein_G_liposomal_nanovesicles_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(05)00655-7 DB - PRIME DP - Unbound Medicine ER -