Tags

Type your tag names separated by a space and hit enter

High-throughput technique for comprehensive analysis of Japanese green tea quality assessment using ultra-performance liquid chromatography with time-of-flight mass spectrometry (UPLC/TOF MS).
J Agric Food Chem. 2008 Nov 26; 56(22):10705-8.JA

Abstract

Applications of metabolomics techniques along with chemometrics provide an understanding in the relationship between metabolome of green tea and its quality. A coupled of ultra-performance liquid chromatography with time-of-flight mass spectrometry (UPLC/TOF MS) allowed a high-throughput and comprehensive analysis with minimal sample preparation. Using this technique, a wide range of metabolites were investigated. Data analysis was rapid, considering that the fingerprinting technique was performed. A set of green tea samples from 2006 tea contest of the Kansai area was analyzed to prove usefulness of the developed technique. Green tea with different qualities were discriminated through principal component analysis (PCA). Consequently, projection to latent structure by means of partial least-squares (PLS) was performed to create a constructive quality-predictive model by means of metabolic fingerprinting. Beside epigallocatechin, other predominant catechins, including epigallocatechin gallate and epicatechin gallate, detected in green tea were found to be significant biomarkers to the high quality of Japanese green tea (Sencha).

Authors+Show Affiliations

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1, Yamadaoka, Suita, Osaka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18973299

Citation

Pongsuwan, Wipawee, et al. "High-throughput Technique for Comprehensive Analysis of Japanese Green Tea Quality Assessment Using Ultra-performance Liquid Chromatography With Time-of-flight Mass Spectrometry (UPLC/TOF MS)." Journal of Agricultural and Food Chemistry, vol. 56, no. 22, 2008, pp. 10705-8.
Pongsuwan W, Bamba T, Harada K, et al. High-throughput technique for comprehensive analysis of Japanese green tea quality assessment using ultra-performance liquid chromatography with time-of-flight mass spectrometry (UPLC/TOF MS). J Agric Food Chem. 2008;56(22):10705-8.
Pongsuwan, W., Bamba, T., Harada, K., Yonetani, T., Kobayashi, A., & Fukusaki, E. (2008). High-throughput technique for comprehensive analysis of Japanese green tea quality assessment using ultra-performance liquid chromatography with time-of-flight mass spectrometry (UPLC/TOF MS). Journal of Agricultural and Food Chemistry, 56(22), 10705-8. https://doi.org/10.1021/jf8018003
Pongsuwan W, et al. High-throughput Technique for Comprehensive Analysis of Japanese Green Tea Quality Assessment Using Ultra-performance Liquid Chromatography With Time-of-flight Mass Spectrometry (UPLC/TOF MS). J Agric Food Chem. 2008 Nov 26;56(22):10705-8. PubMed PMID: 18973299.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-throughput technique for comprehensive analysis of Japanese green tea quality assessment using ultra-performance liquid chromatography with time-of-flight mass spectrometry (UPLC/TOF MS). AU - Pongsuwan,Wipawee, AU - Bamba,Takeshi, AU - Harada,Kazuo, AU - Yonetani,Tsutomu, AU - Kobayashi,Akio, AU - Fukusaki,Eiichiro, PY - 2008/11/1/pubmed PY - 2009/4/4/medline PY - 2008/11/1/entrez SP - 10705 EP - 8 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 56 IS - 22 N2 - Applications of metabolomics techniques along with chemometrics provide an understanding in the relationship between metabolome of green tea and its quality. A coupled of ultra-performance liquid chromatography with time-of-flight mass spectrometry (UPLC/TOF MS) allowed a high-throughput and comprehensive analysis with minimal sample preparation. Using this technique, a wide range of metabolites were investigated. Data analysis was rapid, considering that the fingerprinting technique was performed. A set of green tea samples from 2006 tea contest of the Kansai area was analyzed to prove usefulness of the developed technique. Green tea with different qualities were discriminated through principal component analysis (PCA). Consequently, projection to latent structure by means of partial least-squares (PLS) was performed to create a constructive quality-predictive model by means of metabolic fingerprinting. Beside epigallocatechin, other predominant catechins, including epigallocatechin gallate and epicatechin gallate, detected in green tea were found to be significant biomarkers to the high quality of Japanese green tea (Sencha). SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/18973299/High_throughput_technique_for_comprehensive_analysis_of_Japanese_green_tea_quality_assessment_using_ultra_performance_liquid_chromatography_with_time_of_flight_mass_spectrometry__UPLC/TOF_MS__ L2 - https://doi.org/10.1021/jf8018003 DB - PRIME DP - Unbound Medicine ER -