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Expression analysis of the molluscan p53 protein family mRNA in mussels (Mytilus spp.) exposed to organic contaminants.
Comp Biochem Physiol C Toxicol Pharmacol. 2009 Apr; 149(3):414-8.CB

Abstract

In this study, we report the tissue expression analysis of the p53 protein family mRNA in mussels (Mytilus spp.) by means of quantitative RT-PCR. The tissue specific response was evaluated after 24 h exposure to a sublethal benzo[a]pyrene (B[a]P) concentration (75 nM), showing a 2.6 fold induction in digestive gland cells and a dramatic gene down regulation in circulating hemocytes. The comet assay and DNA gel diffusion tests showed significant effects in hemocytes and negligible differences in the digestive gland nuclei, implicating p53 in DNA damage of molluscan hemocytes. Finally, the kinetics of p53 protein family mRNA expression in the digestive gland of animals exposed to B[a]P and crude oil (0.5 ppm) showed partially overlapping trends, characterised by a common down regulation after 1 week exposure. These data should be carefully considered in view of the biological effects of organic pollutants and particularly following spills.

Authors+Show Affiliations

Department of Environmental and Life Sciences, University of Piemonte Orientale Amedeo Avogadro, Via Bellini 25 G, 15100 Alessandria, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18973830

Citation

Banni, Mohamed, et al. "Expression Analysis of the Molluscan P53 Protein Family mRNA in Mussels (Mytilus Spp.) Exposed to Organic Contaminants." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 149, no. 3, 2009, pp. 414-8.
Banni M, Negri A, Rebelo M, et al. Expression analysis of the molluscan p53 protein family mRNA in mussels (Mytilus spp.) exposed to organic contaminants. Comp Biochem Physiol C Toxicol Pharmacol. 2009;149(3):414-8.
Banni, M., Negri, A., Rebelo, M., Rapallo, F., Boussetta, H., Viarengo, A., & Dondero, F. (2009). Expression analysis of the molluscan p53 protein family mRNA in mussels (Mytilus spp.) exposed to organic contaminants. Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 149(3), 414-8. https://doi.org/10.1016/j.cbpc.2008.09.017
Banni M, et al. Expression Analysis of the Molluscan P53 Protein Family mRNA in Mussels (Mytilus Spp.) Exposed to Organic Contaminants. Comp Biochem Physiol C Toxicol Pharmacol. 2009;149(3):414-8. PubMed PMID: 18973830.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression analysis of the molluscan p53 protein family mRNA in mussels (Mytilus spp.) exposed to organic contaminants. AU - Banni,Mohamed, AU - Negri,Alessandro, AU - Rebelo,Mauro, AU - Rapallo,Fabio, AU - Boussetta,Hamadi, AU - Viarengo,Aldo, AU - Dondero,Francesco, Y1 - 2008/10/11/ PY - 2008/07/22/received PY - 2008/09/29/revised PY - 2008/09/29/accepted PY - 2008/11/1/pubmed PY - 2009/6/16/medline PY - 2008/11/1/entrez SP - 414 EP - 8 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 149 IS - 3 N2 - In this study, we report the tissue expression analysis of the p53 protein family mRNA in mussels (Mytilus spp.) by means of quantitative RT-PCR. The tissue specific response was evaluated after 24 h exposure to a sublethal benzo[a]pyrene (B[a]P) concentration (75 nM), showing a 2.6 fold induction in digestive gland cells and a dramatic gene down regulation in circulating hemocytes. The comet assay and DNA gel diffusion tests showed significant effects in hemocytes and negligible differences in the digestive gland nuclei, implicating p53 in DNA damage of molluscan hemocytes. Finally, the kinetics of p53 protein family mRNA expression in the digestive gland of animals exposed to B[a]P and crude oil (0.5 ppm) showed partially overlapping trends, characterised by a common down regulation after 1 week exposure. These data should be carefully considered in view of the biological effects of organic pollutants and particularly following spills. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/18973830/Expression_analysis_of_the_molluscan_p53_protein_family_mRNA_in_mussels__Mytilus_spp___exposed_to_organic_contaminants_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(08)00189-0 DB - PRIME DP - Unbound Medicine ER -