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A new epicatechin gallate and calpain inhibitory activity from Orostachys japonicus.
Fitoterapia. 2009 Jan; 80(1):73-6.F

Abstract

Calpains are calcium-dependent proteases that cleave a variety of intracellular substrates. The overactivation of mu-calpain is associated with a wide range of disease conditions. To search for calpain inhibitors from natural products, the phytochemical constituents of the ethyl acetate fraction of the whole plant of Orostachys japonicus (Crassulaceae) were studied. The various chromatographic separation of this fraction led to the isolation of a new tannin, (-)-epicatechin 5-gallate (1) along with 9 known compounds. Their structures were elucidated by spectroscopic and chemical analyses. Among them, (-)-epicatechin 5-gallate (1) and kaempferol (9) exhibited moderate inhibitory activity against mu-calpain with IC(50) values of 18.0+/-2.9 and 15.4+/-2.0 microg/ml, respectively.

Authors+Show Affiliations

Bioanalysis and Biotransformation Research Center, Life Sciences Research Division, Korea Institute of Science & Technology, PO Box 131, Cheongryang, Seoul 130-650, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18977282

Citation

Kim, Hyoung Ja, et al. "A New Epicatechin Gallate and Calpain Inhibitory Activity From Orostachys Japonicus." Fitoterapia, vol. 80, no. 1, 2009, pp. 73-6.
Kim HJ, Lee JY, Kim SM, et al. A new epicatechin gallate and calpain inhibitory activity from Orostachys japonicus. Fitoterapia. 2009;80(1):73-6.
Kim, H. J., Lee, J. Y., Kim, S. M., Park, D. A., Jin, C., Hong, S. P., & Lee, Y. S. (2009). A new epicatechin gallate and calpain inhibitory activity from Orostachys japonicus. Fitoterapia, 80(1), 73-6. https://doi.org/10.1016/j.fitote.2008.10.003
Kim HJ, et al. A New Epicatechin Gallate and Calpain Inhibitory Activity From Orostachys Japonicus. Fitoterapia. 2009;80(1):73-6. PubMed PMID: 18977282.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A new epicatechin gallate and calpain inhibitory activity from Orostachys japonicus. AU - Kim,Hyoung Ja, AU - Lee,Jun Young, AU - Kim,Sung Min, AU - Park,Da-Ae, AU - Jin,Changbae, AU - Hong,Seon Pyo, AU - Lee,Yong Sup, Y1 - 2008/10/18/ PY - 2008/09/17/received PY - 2008/10/08/revised PY - 2008/10/08/accepted PY - 2008/11/4/pubmed PY - 2009/4/9/medline PY - 2008/11/4/entrez SP - 73 EP - 6 JF - Fitoterapia JO - Fitoterapia VL - 80 IS - 1 N2 - Calpains are calcium-dependent proteases that cleave a variety of intracellular substrates. The overactivation of mu-calpain is associated with a wide range of disease conditions. To search for calpain inhibitors from natural products, the phytochemical constituents of the ethyl acetate fraction of the whole plant of Orostachys japonicus (Crassulaceae) were studied. The various chromatographic separation of this fraction led to the isolation of a new tannin, (-)-epicatechin 5-gallate (1) along with 9 known compounds. Their structures were elucidated by spectroscopic and chemical analyses. Among them, (-)-epicatechin 5-gallate (1) and kaempferol (9) exhibited moderate inhibitory activity against mu-calpain with IC(50) values of 18.0+/-2.9 and 15.4+/-2.0 microg/ml, respectively. SN - 1873-6971 UR - https://www.unboundmedicine.com/medline/citation/18977282/A_new_epicatechin_gallate_and_calpain_inhibitory_activity_from_Orostachys_japonicus_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0367-326X(08)00210-4 DB - PRIME DP - Unbound Medicine ER -