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Virus-like antigen associated with hepatitis A: investigations in children's acute hepatitis.
Boll Ist Sieroter Milan 1976; 55(5):363-70BI

Abstract

Stool specimens, collected from 8 children with viral hepatitis [5 of type A, presumably, and 3 of type B (HBsAg-positive)] during the acute phase, were examined in the electron microscope. The presence of 27-nm virus-like particles, aggregated by the convalescent serum of a chimpanzee infected with MS-1 and not by the serum of the same animal drawn before infection, was detected by immune electron microscopy (IEM) in the stools of 3 out of the 5 children with suspected hepatitis A. The virus-like particles (HA-Ag) present in the stools were aggregated by the serum drawn from the corresponding subject during convalescence and not by that obtained in the acute phase of the disease. Employing as antigen a stool particle preparation purified by isopycnic banding in cesium cloride, it was pointed out that, in all 5 children with suspected hepatitis A, antibodies capable to cause the aggregation of HA-Ag particles, appeared. Anti-HA antibodies turned out to be present also in three lots of commercial immunoglobulins. In the three subjects with type B hepatitis (HBsAg-positive) neither virus-like particles with a 27-nm diameter were observed, nor anti-HA antibodies appeared in the convalescent serum.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

189787

Citation

Crovari, P, et al. "Virus-like Antigen Associated With Hepatitis A: Investigations in Children's Acute Hepatitis." Bollettino dell'Istituto Sieroterapico Milanese, vol. 55, no. 5, 1976, pp. 363-70.
Crovari P, Cuneo-Crovari P, Mistretta AP, et al. Virus-like antigen associated with hepatitis A: investigations in children's acute hepatitis. Boll Ist Sieroter Milan. 1976;55(5):363-70.
Crovari, P., Cuneo-Crovari, P., Mistretta, A. P., & Tolentino, P. (1976). Virus-like antigen associated with hepatitis A: investigations in children's acute hepatitis. Bollettino dell'Istituto Sieroterapico Milanese, 55(5), pp. 363-70.
Crovari P, et al. Virus-like Antigen Associated With Hepatitis A: Investigations in Children's Acute Hepatitis. Boll Ist Sieroter Milan. 1976;55(5):363-70. PubMed PMID: 189787.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Virus-like antigen associated with hepatitis A: investigations in children's acute hepatitis. AU - Crovari,P, AU - Cuneo-Crovari,P, AU - Mistretta,A P, AU - Tolentino,P, PY - 1976/1/1/pubmed PY - 1976/1/1/medline PY - 1976/1/1/entrez SP - 363 EP - 70 JF - Bollettino dell'Istituto sieroterapico milanese JO - Boll Ist Sieroter Milan VL - 55 IS - 5 N2 - Stool specimens, collected from 8 children with viral hepatitis [5 of type A, presumably, and 3 of type B (HBsAg-positive)] during the acute phase, were examined in the electron microscope. The presence of 27-nm virus-like particles, aggregated by the convalescent serum of a chimpanzee infected with MS-1 and not by the serum of the same animal drawn before infection, was detected by immune electron microscopy (IEM) in the stools of 3 out of the 5 children with suspected hepatitis A. The virus-like particles (HA-Ag) present in the stools were aggregated by the serum drawn from the corresponding subject during convalescence and not by that obtained in the acute phase of the disease. Employing as antigen a stool particle preparation purified by isopycnic banding in cesium cloride, it was pointed out that, in all 5 children with suspected hepatitis A, antibodies capable to cause the aggregation of HA-Ag particles, appeared. Anti-HA antibodies turned out to be present also in three lots of commercial immunoglobulins. In the three subjects with type B hepatitis (HBsAg-positive) neither virus-like particles with a 27-nm diameter were observed, nor anti-HA antibodies appeared in the convalescent serum. SN - 0021-2547 UR - https://www.unboundmedicine.com/medline/citation/189787/Virus_like_antigen_associated_with_hepatitis_A:_investigations_in_children's_acute_hepatitis_ L2 - http://www.diseaseinfosearch.org/result/3332 DB - PRIME DP - Unbound Medicine ER -