Tags

Type your tag names separated by a space and hit enter

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry monitoring of anthocyanins in extracts from Arabidopsis thaliana leaves.
Rapid Commun Mass Spectrom. 2008 Dec; 22(23):3949-56.RC

Abstract

Anthocyanins are secondary plant metabolites ubiquitous in the plant kingdom. They have different biological activities, so monitoring their content in plant tissue or in feed/food derived from plants may be an important task in different projects from various fields of molecular biology and biotechnology. Profiling of secondary metabolites with high-performance liquid chromatography/mass spectrometry (HPLC/MS) systems is time-consuming, especially when many samples have to be checked within a defined time frame with a reasonable number of repetitions according to the metabolomic standards. Even application of the advanced ultra-performance liquid chromatography (UPLC)/MS or equivalent systems would require a long time for analysis of numerous samples. We demonstrate the applicability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the assessment of level (concentration) of anthocyanins in leaf tissues of four Arabidopsis thaliana ecotypes grown at normal (20 degrees C/16 degrees C day/night) and decreased (4 degrees C) temperature. The quantitative results were obtained for anthocyanins with MALDI-TOF MS using ferulic acid as a matrix. The amounts of anthocyanins in leaves of A. thaliana varied from 0.3-2.5 microg per gram of leaves for ecotypes Col-0 and C24, respectively, and contents of these markedly increased in plants grown in the cold. The applied analytical method exhibited better repeatability of measurements than obtained with an HPLC/ion trap MS system.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Marczak L
Institute of Bioorganic Chemistry PAS, Noskowskiego 12/14, 61-704 Poznań, Poland.
Kachlicki P
No affiliation info available
Koźniewski P
No affiliation info available
Skirycz A
No affiliation info available
Krajewski P
No affiliation info available
Stobiecki M
No affiliation info available

MeSH

AnthocyaninsArabidopsisCoumaric AcidsPlant ExtractsPlant LeavesReproducibility of ResultsSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18980256

Citation

Marczak, Lukasz, et al. "Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry Monitoring of Anthocyanins in Extracts From Arabidopsis Thaliana Leaves." Rapid Communications in Mass Spectrometry : RCM, vol. 22, no. 23, 2008, pp. 3949-56.
Marczak L, Kachlicki P, Koźniewski P, et al. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry monitoring of anthocyanins in extracts from Arabidopsis thaliana leaves. Rapid Commun Mass Spectrom. 2008;22(23):3949-56.
Marczak, L., Kachlicki, P., Koźniewski, P., Skirycz, A., Krajewski, P., & Stobiecki, M. (2008). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry monitoring of anthocyanins in extracts from Arabidopsis thaliana leaves. Rapid Communications in Mass Spectrometry : RCM, 22(23), 3949-56. https://doi.org/10.1002/rcm.3819
Marczak L, et al. Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry Monitoring of Anthocyanins in Extracts From Arabidopsis Thaliana Leaves. Rapid Commun Mass Spectrom. 2008;22(23):3949-56. PubMed PMID: 18980256.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry monitoring of anthocyanins in extracts from Arabidopsis thaliana leaves. AU - Marczak,Lukasz, AU - Kachlicki,Piotr, AU - Koźniewski,Piotr, AU - Skirycz,Aleksandra, AU - Krajewski,Paweł, AU - Stobiecki,Maciej, PY - 2008/11/5/pubmed PY - 2009/5/9/medline PY - 2008/11/5/entrez SP - 3949 EP - 56 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 22 IS - 23 N2 - Anthocyanins are secondary plant metabolites ubiquitous in the plant kingdom. They have different biological activities, so monitoring their content in plant tissue or in feed/food derived from plants may be an important task in different projects from various fields of molecular biology and biotechnology. Profiling of secondary metabolites with high-performance liquid chromatography/mass spectrometry (HPLC/MS) systems is time-consuming, especially when many samples have to be checked within a defined time frame with a reasonable number of repetitions according to the metabolomic standards. Even application of the advanced ultra-performance liquid chromatography (UPLC)/MS or equivalent systems would require a long time for analysis of numerous samples. We demonstrate the applicability of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the assessment of level (concentration) of anthocyanins in leaf tissues of four Arabidopsis thaliana ecotypes grown at normal (20 degrees C/16 degrees C day/night) and decreased (4 degrees C) temperature. The quantitative results were obtained for anthocyanins with MALDI-TOF MS using ferulic acid as a matrix. The amounts of anthocyanins in leaves of A. thaliana varied from 0.3-2.5 microg per gram of leaves for ecotypes Col-0 and C24, respectively, and contents of these markedly increased in plants grown in the cold. The applied analytical method exhibited better repeatability of measurements than obtained with an HPLC/ion trap MS system. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/18980256/Matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_monitoring_of_anthocyanins_in_extracts_from_Arabidopsis_thaliana_leaves_ DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓7]

Related Citations

More