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Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to rapidly screen for albuminuria.
Rapid Commun Mass Spectrom. 2008 Dec; 22(23):3754-60.RC

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is used as an alternative method for the rapid diagnosis of albuminuria. This technique requires no further sample pretreatment than simply mixing the urine sample with a MALDI matrix and drying under ambient conditions. The resulting MALDI mass spectra reveal albumin ions having charges ranging from +1 to +5. The detection of albumin is possible using any of the three most common MALDI matrices - sinapinic acid (SA), 2,5-dihydroxybenzoic acid (2,5-DHB), or 4-hydroxy-alpha-cyanocinnamic acid (alpha-CHC). Using this analytical approach, the limit of detection for albumin in urine is 10(-6) M, approximately 5 to 10 times lower than that detectable through conventional chemical testing.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Shiea J
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan. jetea@mail.nsysu.edu.tw
Cho YT
No affiliation info available
Lin YH
No affiliation info available
Chang CW
No affiliation info available
Lo LH
No affiliation info available
Lee YC
No affiliation info available
Ke HL
No affiliation info available
Wu WJ
No affiliation info available
Wu DC
No affiliation info available

MeSH

AdultAlbuminuriaCoumaric AcidsFemaleGentisatesHumansMaleMiddle AgedSensitivity and SpecificitySpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationYoung Adult

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18980259

Citation

Shiea, Jentaie, et al. "Using Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry to Rapidly Screen for Albuminuria." Rapid Communications in Mass Spectrometry : RCM, vol. 22, no. 23, 2008, pp. 3754-60.
Shiea J, Cho YT, Lin YH, et al. Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to rapidly screen for albuminuria. Rapid Commun Mass Spectrom. 2008;22(23):3754-60.
Shiea, J., Cho, Y. T., Lin, Y. H., Chang, C. W., Lo, L. H., Lee, Y. C., Ke, H. L., Wu, W. J., & Wu, D. C. (2008). Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to rapidly screen for albuminuria. Rapid Communications in Mass Spectrometry : RCM, 22(23), 3754-60. https://doi.org/10.1002/rcm.3792
Shiea J, et al. Using Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry to Rapidly Screen for Albuminuria. Rapid Commun Mass Spectrom. 2008;22(23):3754-60. PubMed PMID: 18980259.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to rapidly screen for albuminuria. AU - Shiea,Jentaie, AU - Cho,Yi-Tzu, AU - Lin,Yen-Hsiu, AU - Chang,Chin-Wen, AU - Lo,Li-Hua, AU - Lee,Yi-Chern, AU - Ke,Hung-Lung, AU - Wu,Wen-Jeng, AU - Wu,Deng-Chyang, PY - 2008/11/5/pubmed PY - 2009/5/9/medline PY - 2008/11/5/entrez SP - 3754 EP - 60 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 22 IS - 23 N2 - Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is used as an alternative method for the rapid diagnosis of albuminuria. This technique requires no further sample pretreatment than simply mixing the urine sample with a MALDI matrix and drying under ambient conditions. The resulting MALDI mass spectra reveal albumin ions having charges ranging from +1 to +5. The detection of albumin is possible using any of the three most common MALDI matrices - sinapinic acid (SA), 2,5-dihydroxybenzoic acid (2,5-DHB), or 4-hydroxy-alpha-cyanocinnamic acid (alpha-CHC). Using this analytical approach, the limit of detection for albumin in urine is 10(-6) M, approximately 5 to 10 times lower than that detectable through conventional chemical testing. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/18980259/Using_matrix_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_to_rapidly_screen_for_albuminuria_ L2 - https://doi.org/10.1002/rcm.3792 DB - PRIME DP - Unbound Medicine ER -