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Adaptive immune responses against parvovirus B19 in patients with myocardial disease.
J Clin Virol. 2009 Jan; 44(1):27-32.JC

Abstract

BACKGROUND

Parvovirus B19 (B19V)-DNA is frequently detected in endomyocardial biopsies (EMBs) from patients with acute myocarditis (AMC) and dilated cardiomyopathy (DCM), but also in various healthy tissues. The clinical relevance of this DNA-persistence is unclear.

OBJECTIVES

To investigate potential pathogenic influences of B19V-DNA in EMBs, we analyzed B19V-specific adaptive immune responses in AMC/DCM patients and healthy controls.

STUDY DESIGN

15 AMC/DCM patients with detectable B19V-DNA in EMBs and 51 controls were analyzed for signs of acute B19V-infections and virus-specific immune responses by PCR, ELISA, Western line, and ELISpot-assays.

RESULTS

Productive B19V-infection was determined in three patients. Slightly lower levels of B19V-specific T-cells were observed in patients as compared to the controls, no differences were observed in virus-specific serology. Viral DNA-load in EMBs could not be correlated to the number of B19V-specific T-cells. No differences in T-cell response, viremia and/or serological markers indicative for viral pathogenesis were observed in patients with inflammatory cardiomyopathy.

CONCLUSIONS

Discrepancies in B19V-specific adaptive immunity were not observed in AMC/DCM patients as compared to controls. The data indicate that the exclusive detection of B19V-DNA in EMBs is not sufficient to associate B19V with AMC/DCM but should be complemented with additional virological and immunological parameters in further studies.

Authors+Show Affiliations

Institute of Medical Microbiology and Hygiene, University of Regensburg, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18980860

Citation

Lindner, Juha, et al. "Adaptive Immune Responses Against Parvovirus B19 in Patients With Myocardial Disease." Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, vol. 44, no. 1, 2009, pp. 27-32.
Lindner J, Noutsias M, Lassner D, et al. Adaptive immune responses against parvovirus B19 in patients with myocardial disease. J Clin Virol. 2009;44(1):27-32.
Lindner, J., Noutsias, M., Lassner, D., Wenzel, J., Schultheiss, H. P., Kuehl, U., & Modrow, S. (2009). Adaptive immune responses against parvovirus B19 in patients with myocardial disease. Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology, 44(1), 27-32. https://doi.org/10.1016/j.jcv.2008.09.007
Lindner J, et al. Adaptive Immune Responses Against Parvovirus B19 in Patients With Myocardial Disease. J Clin Virol. 2009;44(1):27-32. PubMed PMID: 18980860.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Adaptive immune responses against parvovirus B19 in patients with myocardial disease. AU - Lindner,Juha, AU - Noutsias,Michel, AU - Lassner,Dirk, AU - Wenzel,Jürgen, AU - Schultheiss,Heinz-Peter, AU - Kuehl,Uwe, AU - Modrow,Susanne, Y1 - 2008/11/05/ PY - 2008/06/27/received PY - 2008/09/02/revised PY - 2008/09/12/accepted PY - 2008/11/5/pubmed PY - 2009/3/11/medline PY - 2008/11/5/entrez SP - 27 EP - 32 JF - Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology JO - J Clin Virol VL - 44 IS - 1 N2 - BACKGROUND: Parvovirus B19 (B19V)-DNA is frequently detected in endomyocardial biopsies (EMBs) from patients with acute myocarditis (AMC) and dilated cardiomyopathy (DCM), but also in various healthy tissues. The clinical relevance of this DNA-persistence is unclear. OBJECTIVES: To investigate potential pathogenic influences of B19V-DNA in EMBs, we analyzed B19V-specific adaptive immune responses in AMC/DCM patients and healthy controls. STUDY DESIGN: 15 AMC/DCM patients with detectable B19V-DNA in EMBs and 51 controls were analyzed for signs of acute B19V-infections and virus-specific immune responses by PCR, ELISA, Western line, and ELISpot-assays. RESULTS: Productive B19V-infection was determined in three patients. Slightly lower levels of B19V-specific T-cells were observed in patients as compared to the controls, no differences were observed in virus-specific serology. Viral DNA-load in EMBs could not be correlated to the number of B19V-specific T-cells. No differences in T-cell response, viremia and/or serological markers indicative for viral pathogenesis were observed in patients with inflammatory cardiomyopathy. CONCLUSIONS: Discrepancies in B19V-specific adaptive immunity were not observed in AMC/DCM patients as compared to controls. The data indicate that the exclusive detection of B19V-DNA in EMBs is not sufficient to associate B19V with AMC/DCM but should be complemented with additional virological and immunological parameters in further studies. SN - 1386-6532 UR - https://www.unboundmedicine.com/medline/citation/18980860/Adaptive_immune_responses_against_parvovirus_B19_in_patients_with_myocardial_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1386-6532(08)00330-2 DB - PRIME DP - Unbound Medicine ER -