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Comparison of bronchoalveolar lavage cytospins and smears in dogs and cats.
J Am Anim Hosp Assoc. 2008 Nov-Dec; 44(6):285-94.JA

Abstract

Differences in the cytological interpretation of bronchoalveolar lavage fluid (BALF) after cytospin preparation (CP) or manual smearing of pelleted cells preparation (MSP) were investigated in client-owned dogs and cats with inflammatory or infectious lower respiratory disease. Bronchoalveolar lavage fluid from healthy cats was also examined. With MSP, cell lysis was more frequently observed, and cellular distribution was more heterogeneous throughout the slide. When samples from healthy and diseased animals were considered together, a significantly greater percentage of neutrophils was seen on CP than on MSP slides (P<0.002). Cytospin preparations were considered of better quality in all individual comparisons. Cytospin preparation is advised in the evaluation of BALF with low total cell count. When only MSPs are evaluated, clinicians should be aware that differential neutrophil counts may underestimate the counts found on CP slides.

Authors+Show Affiliations

Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Liège, Liège, 4000 Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18981193

Citation

Dehard, Sandrine, et al. "Comparison of Bronchoalveolar Lavage Cytospins and Smears in Dogs and Cats." Journal of the American Animal Hospital Association, vol. 44, no. 6, 2008, pp. 285-94.
Dehard S, Bernaerts F, Peeters D, et al. Comparison of bronchoalveolar lavage cytospins and smears in dogs and cats. J Am Anim Hosp Assoc. 2008;44(6):285-94.
Dehard, S., Bernaerts, F., Peeters, D., Detilleux, J., McEntee, K., Day, M. J., & Clercx, C. (2008). Comparison of bronchoalveolar lavage cytospins and smears in dogs and cats. Journal of the American Animal Hospital Association, 44(6), 285-94.
Dehard S, et al. Comparison of Bronchoalveolar Lavage Cytospins and Smears in Dogs and Cats. J Am Anim Hosp Assoc. 2008 Nov-Dec;44(6):285-94. PubMed PMID: 18981193.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of bronchoalveolar lavage cytospins and smears in dogs and cats. AU - Dehard,Sandrine, AU - Bernaerts,Frederique, AU - Peeters,Dominique, AU - Detilleux,Johann, AU - McEntee,Kathleen, AU - Day,Michael J, AU - Clercx,Cecile, PY - 2008/11/5/pubmed PY - 2009/2/10/medline PY - 2008/11/5/entrez SP - 285 EP - 94 JF - Journal of the American Animal Hospital Association JO - J Am Anim Hosp Assoc VL - 44 IS - 6 N2 - Differences in the cytological interpretation of bronchoalveolar lavage fluid (BALF) after cytospin preparation (CP) or manual smearing of pelleted cells preparation (MSP) were investigated in client-owned dogs and cats with inflammatory or infectious lower respiratory disease. Bronchoalveolar lavage fluid from healthy cats was also examined. With MSP, cell lysis was more frequently observed, and cellular distribution was more heterogeneous throughout the slide. When samples from healthy and diseased animals were considered together, a significantly greater percentage of neutrophils was seen on CP than on MSP slides (P<0.002). Cytospin preparations were considered of better quality in all individual comparisons. Cytospin preparation is advised in the evaluation of BALF with low total cell count. When only MSPs are evaluated, clinicians should be aware that differential neutrophil counts may underestimate the counts found on CP slides. SN - 1547-3317 UR - https://www.unboundmedicine.com/medline/citation/18981193/Comparison_of_bronchoalveolar_lavage_cytospins_and_smears_in_dogs_and_cats_ L2 - http://jaaha.org/doi/10.5326/0440285?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -