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Label-free fluorescence detection in capillary and microchip electrophoresis.
Anal Bioanal Chem. 2009 Jan; 393(2):515-25.AB

Abstract

Herein, we summarize the current status of native fluorescence detection in microchannel electrophoresis, with a strong focus on chip-based systems. Fluorescence detection is a powerful technique with unsurpassed sensitivity down to the single-molecule level. Accordingly fluorescence detection is attractive in combination with miniaturised separation techniques. A drawback is, however, the need to derivatize most analytes prior to analysis. This can often be circumvented by utilising excitation light in the UV spectral range in order to excite intrinsic fluorescence. As sensitive absorbance detection is challenging in chip-based systems, deep-UV fluorescence detection is currently one of the most general optical detection techniques in microchip electrophoresis, which is especially attractive for the detection of unlabelled proteins. This review gives an overview of research on native fluorescence detection in capillary (CE) and microchip electrophoresis (MCE) between 1998 and 2008. It discusses material aspects of native fluorescence detection and the instrumentation used, with particular focus on the detector design. Newer developments, featured techniques, and their prospects in the future are also included. In the last section, applications in bioanalysis, drug determination, and environmental analysis are reviewed with regard to limits of detection.

Authors+Show Affiliations

Institute of Analytical Chemistry, Universitat Leipzig, Johannisallee 29, 04103, Leipzig, Germany.No affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

18982318

Citation

Schulze, Philipp, and Detlev Belder. "Label-free Fluorescence Detection in Capillary and Microchip Electrophoresis." Analytical and Bioanalytical Chemistry, vol. 393, no. 2, 2009, pp. 515-25.
Schulze P, Belder D. Label-free fluorescence detection in capillary and microchip electrophoresis. Anal Bioanal Chem. 2009;393(2):515-25.
Schulze, P., & Belder, D. (2009). Label-free fluorescence detection in capillary and microchip electrophoresis. Analytical and Bioanalytical Chemistry, 393(2), 515-25. https://doi.org/10.1007/s00216-008-2452-7
Schulze P, Belder D. Label-free Fluorescence Detection in Capillary and Microchip Electrophoresis. Anal Bioanal Chem. 2009;393(2):515-25. PubMed PMID: 18982318.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Label-free fluorescence detection in capillary and microchip electrophoresis. AU - Schulze,Philipp, AU - Belder,Detlev, Y1 - 2008/11/01/ PY - 2008/08/04/received PY - 2008/10/01/accepted PY - 2008/09/18/revised PY - 2008/11/5/pubmed PY - 2009/2/12/medline PY - 2008/11/5/entrez SP - 515 EP - 25 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 393 IS - 2 N2 - Herein, we summarize the current status of native fluorescence detection in microchannel electrophoresis, with a strong focus on chip-based systems. Fluorescence detection is a powerful technique with unsurpassed sensitivity down to the single-molecule level. Accordingly fluorescence detection is attractive in combination with miniaturised separation techniques. A drawback is, however, the need to derivatize most analytes prior to analysis. This can often be circumvented by utilising excitation light in the UV spectral range in order to excite intrinsic fluorescence. As sensitive absorbance detection is challenging in chip-based systems, deep-UV fluorescence detection is currently one of the most general optical detection techniques in microchip electrophoresis, which is especially attractive for the detection of unlabelled proteins. This review gives an overview of research on native fluorescence detection in capillary (CE) and microchip electrophoresis (MCE) between 1998 and 2008. It discusses material aspects of native fluorescence detection and the instrumentation used, with particular focus on the detector design. Newer developments, featured techniques, and their prospects in the future are also included. In the last section, applications in bioanalysis, drug determination, and environmental analysis are reviewed with regard to limits of detection. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/18982318/Label_free_fluorescence_detection_in_capillary_and_microchip_electrophoresis_ L2 - https://dx.doi.org/10.1007/s00216-008-2452-7 DB - PRIME DP - Unbound Medicine ER -