Tags

Type your tag names separated by a space and hit enter

CD4 homologues in Atlantic salmon.
Fish Shellfish Immunol 2009; 26(1):10-8FS

Abstract

In mammals CD4 is a membrane glycoprotein on Th cells with four extracellular immunoglobulin-like (Ig-like) domains (D1-D4). It functions as a co-receptor during immune recognition between the TCR and the MHC II/peptide complex. The cytoplasmic domain binds p56lck, a protein kinase responsible for phosphorylating CD3 which is the first interaction in a cascade leading to T cell activation. We have previously reported a CD4-2 gene in rainbow trout (Oncorhynchus mykiss) which was found adjacent to the CD4-1 gene by synteny analysis. There are two subtypes (a and b) of CD4-2 in rainbow trout, with two Ig-like extracellular domains. Here we present the homologues of mammalian CD4 in Atlantic salmon (Salmo salar): CD4-1 with four extracellular domains and CD4-2a and CD4-2b with two extracellular domains. A Southern blot analysis shows two copies of the CD4-1 gene in the genomic DNA of the closely related rainbow trout. The genes for CD4-1 and CD4-2 have been sequenced and show typical traits for CD4 genes, such as the code for the first domain (D1) being divided between two exons and the other domains being largely coded for by single exons. The corresponding translated cDNAs show little (13-17%) identity to higher vertebrates and are approximately 37% similar to other translated, teleost sequences but are 89% identical to the closely related rainbow trout. However they exhibit conserved features such as the Lck binding motif in their cytoplasmic domains and the order of variable and constant type Ig-like domains. qRT-PCR data are presented describing the differential tissue expression of these genes together with other T cell markers (TCR and CD3) in several individuals.

Authors+Show Affiliations

Department of Biology, University of Bergen, Bergen, Norway. lindsey.moore@bio.uib.noNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18983924

Citation

Moore, L J., et al. "CD4 Homologues in Atlantic Salmon." Fish & Shellfish Immunology, vol. 26, no. 1, 2009, pp. 10-8.
Moore LJ, Dijkstra JM, Koppang EO, et al. CD4 homologues in Atlantic salmon. Fish Shellfish Immunol. 2009;26(1):10-8.
Moore, L. J., Dijkstra, J. M., Koppang, E. O., & Hordvik, I. (2009). CD4 homologues in Atlantic salmon. Fish & Shellfish Immunology, 26(1), pp. 10-8. doi:10.1016/j.fsi.2008.09.019.
Moore LJ, et al. CD4 Homologues in Atlantic Salmon. Fish Shellfish Immunol. 2009;26(1):10-8. PubMed PMID: 18983924.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD4 homologues in Atlantic salmon. AU - Moore,L J, AU - Dijkstra,J M, AU - Koppang,E O, AU - Hordvik,I, Y1 - 2008/10/15/ PY - 2008/06/29/received PY - 2008/09/25/revised PY - 2008/09/25/accepted PY - 2008/11/6/pubmed PY - 2009/3/3/medline PY - 2008/11/6/entrez SP - 10 EP - 8 JF - Fish & shellfish immunology JO - Fish Shellfish Immunol. VL - 26 IS - 1 N2 - In mammals CD4 is a membrane glycoprotein on Th cells with four extracellular immunoglobulin-like (Ig-like) domains (D1-D4). It functions as a co-receptor during immune recognition between the TCR and the MHC II/peptide complex. The cytoplasmic domain binds p56lck, a protein kinase responsible for phosphorylating CD3 which is the first interaction in a cascade leading to T cell activation. We have previously reported a CD4-2 gene in rainbow trout (Oncorhynchus mykiss) which was found adjacent to the CD4-1 gene by synteny analysis. There are two subtypes (a and b) of CD4-2 in rainbow trout, with two Ig-like extracellular domains. Here we present the homologues of mammalian CD4 in Atlantic salmon (Salmo salar): CD4-1 with four extracellular domains and CD4-2a and CD4-2b with two extracellular domains. A Southern blot analysis shows two copies of the CD4-1 gene in the genomic DNA of the closely related rainbow trout. The genes for CD4-1 and CD4-2 have been sequenced and show typical traits for CD4 genes, such as the code for the first domain (D1) being divided between two exons and the other domains being largely coded for by single exons. The corresponding translated cDNAs show little (13-17%) identity to higher vertebrates and are approximately 37% similar to other translated, teleost sequences but are 89% identical to the closely related rainbow trout. However they exhibit conserved features such as the Lck binding motif in their cytoplasmic domains and the order of variable and constant type Ig-like domains. qRT-PCR data are presented describing the differential tissue expression of these genes together with other T cell markers (TCR and CD3) in several individuals. SN - 1095-9947 UR - https://www.unboundmedicine.com/medline/citation/18983924/CD4_homologues_in_Atlantic_salmon_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1050-4648(08)00226-X DB - PRIME DP - Unbound Medicine ER -