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Preparative isolation and purification of two amides from Mallotus lianus Croiz by high-speed counter-current chromatography.
J Sep Sci. 2008 Dec; 31(22):3930-5.JS

Abstract

High-speed counter-current chromatography (HSCCC) was applied to the preparative isolation and purification of two amides from Mallotus lianus Croiz. In a single HSCCC separation, using the two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (5:1:5:1 v/v), 247.5 mg of the enriched crude sample was separated to afford 10.3 mg of N-isobutyl-2E,4E,12Z-octadecatrienamide and 15.7 mg of (7Z,10Z,18Z)tricosa-7,10,18-trienamide, a novel compound, with the purities of 98.0 and 94.6%, respectively. The HSCCC fractions were analyzed by HPLC and chemical structures of the compounds were identified by 1D- and 2D-NMR, ESI-, and GC-MS.

Authors+Show Affiliations

Department of Chemistry, Zhejiang University, Hangzhou, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18985666

Citation

Jiang, Liyan, et al. "Preparative Isolation and Purification of Two Amides From Mallotus Lianus Croiz By High-speed Counter-current Chromatography." Journal of Separation Science, vol. 31, no. 22, 2008, pp. 3930-5.
Jiang L, Lu Y, He S, et al. Preparative isolation and purification of two amides from Mallotus lianus Croiz by high-speed counter-current chromatography. J Sep Sci. 2008;31(22):3930-5.
Jiang, L., Lu, Y., He, S., Pan, Y., Sun, C., & Wu, T. (2008). Preparative isolation and purification of two amides from Mallotus lianus Croiz by high-speed counter-current chromatography. Journal of Separation Science, 31(22), 3930-5. https://doi.org/10.1002/jssc.200800396
Jiang L, et al. Preparative Isolation and Purification of Two Amides From Mallotus Lianus Croiz By High-speed Counter-current Chromatography. J Sep Sci. 2008;31(22):3930-5. PubMed PMID: 18985666.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparative isolation and purification of two amides from Mallotus lianus Croiz by high-speed counter-current chromatography. AU - Jiang,Liyan, AU - Lu,Yanbin, AU - He,Shan, AU - Pan,Yuanjiang, AU - Sun,Cuirong, AU - Wu,Tianxing, PY - 2008/11/6/entrez PY - 2008/11/6/pubmed PY - 2009/1/23/medline SP - 3930 EP - 5 JF - Journal of separation science JO - J Sep Sci VL - 31 IS - 22 N2 - High-speed counter-current chromatography (HSCCC) was applied to the preparative isolation and purification of two amides from Mallotus lianus Croiz. In a single HSCCC separation, using the two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (5:1:5:1 v/v), 247.5 mg of the enriched crude sample was separated to afford 10.3 mg of N-isobutyl-2E,4E,12Z-octadecatrienamide and 15.7 mg of (7Z,10Z,18Z)tricosa-7,10,18-trienamide, a novel compound, with the purities of 98.0 and 94.6%, respectively. The HSCCC fractions were analyzed by HPLC and chemical structures of the compounds were identified by 1D- and 2D-NMR, ESI-, and GC-MS. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/18985666/Preparative_isolation_and_purification_of_two_amides_from_Mallotus_lianus_Croiz_by_high_speed_counter_current_chromatography_ L2 - https://doi.org/10.1002/jssc.200800396 DB - PRIME DP - Unbound Medicine ER -