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Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.).
Theor Appl Genet. 2009 Feb; 118(3):525-39.TA

Abstract

Low-molecular-weight glutenin subunit (LMW-GS) Glu-B3 has a significant influence on the processing quality of the end-use products of common wheat. To characterize the LMW-GS genes at the Glu-B3 locus, gene-specific PCR primers were designed to amplify eight near-isogenic lines and Cheyenne with different Glu-B3 alleles (a, b, c, d, e, f, g, h and i) defined by protein electrophoretic mobility. The complete coding regions of four Glu-B3 genes with complete coding sequence were obtained and designated as GluB3-1, GluB3-2, GluB3-3 and GluB3-4. Ten allele-specific PCR markers designed from the SNPs present in the sequenced variants discriminated the Glu-B3 proteins of electrophoretic mobility alleles a, b, c, d, e, f, g, h and i. These markers were validated on 161 wheat varieties and advanced lines with different Glu-B3 alleles, thus confirming that the markers can be used in marker-assisted breeding for wheat grain processing quality.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Wang LH
Institute of Crop Science, National Wheat Improvement Centre/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, 100081, Beijing, China.
Zhao XL
No affiliation info available
He ZH
No affiliation info available
Ma W
No affiliation info available
Appels R
No affiliation info available
Peña RJ
No affiliation info available
Xia XC
No affiliation info available

MeSH

AllelesChromosomes, PlantGenes, PlantGenetic MarkersGlutensHaplotypesPolymerase Chain ReactionPolymorphism, Single NucleotideProtein SubunitsSequence Analysis, ProteinTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18989655

Citation

Wang, L H., et al. "Characterization of Low-molecular-weight Glutenin Subunit Glu-B3 Genes and Development of STS Markers in Common Wheat (Triticum Aestivum L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 118, no. 3, 2009, pp. 525-39.
Wang LH, Zhao XL, He ZH, et al. Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.). Theor Appl Genet. 2009;118(3):525-39.
Wang, L. H., Zhao, X. L., He, Z. H., Ma, W., Appels, R., Peña, R. J., & Xia, X. C. (2009). Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 118(3), 525-39. https://doi.org/10.1007/s00122-008-0918-9
Wang LH, et al. Characterization of Low-molecular-weight Glutenin Subunit Glu-B3 Genes and Development of STS Markers in Common Wheat (Triticum Aestivum L.). Theor Appl Genet. 2009;118(3):525-39. PubMed PMID: 18989655.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.). AU - Wang,L H, AU - Zhao,X L, AU - He,Z H, AU - Ma,W, AU - Appels,R, AU - Peña,R J, AU - Xia,X C, Y1 - 2008/11/07/ PY - 2008/06/15/received PY - 2008/10/14/accepted PY - 2008/11/8/pubmed PY - 2009/6/25/medline PY - 2008/11/8/entrez SP - 525 EP - 39 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 118 IS - 3 N2 - Low-molecular-weight glutenin subunit (LMW-GS) Glu-B3 has a significant influence on the processing quality of the end-use products of common wheat. To characterize the LMW-GS genes at the Glu-B3 locus, gene-specific PCR primers were designed to amplify eight near-isogenic lines and Cheyenne with different Glu-B3 alleles (a, b, c, d, e, f, g, h and i) defined by protein electrophoretic mobility. The complete coding regions of four Glu-B3 genes with complete coding sequence were obtained and designated as GluB3-1, GluB3-2, GluB3-3 and GluB3-4. Ten allele-specific PCR markers designed from the SNPs present in the sequenced variants discriminated the Glu-B3 proteins of electrophoretic mobility alleles a, b, c, d, e, f, g, h and i. These markers were validated on 161 wheat varieties and advanced lines with different Glu-B3 alleles, thus confirming that the markers can be used in marker-assisted breeding for wheat grain processing quality. SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/18989655/Characterization_of_low_molecular_weight_glutenin_subunit_Glu_B3_genes_and_development_of_STS_markers_in_common_wheat__Triticum_aestivum_L___ L2 - https://dx.doi.org/10.1007/s00122-008-0918-9 DB - PRIME DP - Unbound Medicine ER -