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Measurement of mycophenolic acid and its glucuronide using a novel rapid liquid chromatography-electrospray ionization tandem mass spectrometry assay.
Clin Biochem. 2009 Jan; 42(1-2):83-90.CB

Abstract

OBJECTIVES

Mycophenolic acid (MPA), the active metabolite of the ester prodrug mycophenolate mofetil is an immunosuppressant which selectively inhibits inosine-monophosphate dehydrogenase. The requirement for therapeutic drug monitoring shown in previous studies raises the necessity of acquiring accurate and sensitive methods to measure MPA and also its metabolite mycophenolic acid glucuronide (MPAG).

DESIGN AND METHODS

We developed a robust, rapid, sensitive and highly specific HPLC-electrospray ionization mass spectrometry method to assay MPA and its metabolite MPAG in human plasma and serum. Ion suppression was investigated by a post column infusion experiment.

RESULTS

Determination of MPA and MPAG were performed during a 3.0-min run time. Multiple calibration curves for the analysis of MPA and MPAG exhibited consistent linearity and reproducibility in the range of 0.05 to 100 mg/L (r>0.999) and 6 to 400 mg/L r>0.998, respectively. Limits of detection were 0.009 mg/L for MPA and 4.5 mg/L for MPAG and lower limits of quantification were 0.011 mg/L for MPA and 4.9 mg/L for MPAG. Interassay imprecision was <6.0% for both substances. Mean recovery was 48.9% (range 43.3-60.0%) for MPA and 112.2% (range 95.0-127.0%) for MPAG. Agreement was relatively good for MPA (n=122) between the presented method and a validated ELISA method (Viva analyzer, Siemens Medicals Solutions Diagnostics, NY). The Passing-Bablok regression line was: EMIT=0.91 (LC-MS/MS)+0.17 [mg/L]; r=0.97.

CONCLUSIONS

This simple, robust and interference-free LC-MS/MS assay allows the rapid and accurate determination of MPA and MPAG in human plasma and other body fluids.

Authors+Show Affiliations

Institut für Laboratoriums- und Transfusionsmedizin, Herz- und Diabeteszentrum Nordrhein-Westfalen, Universitätsklinik der Ruhr-Universität Bochum, Georgstrasse 11, 32545 Bad Oeynhausen, Germany. jkuhn@hdz-nrw.deNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18996104

Citation

Kuhn, Joachim, et al. "Measurement of Mycophenolic Acid and Its Glucuronide Using a Novel Rapid Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry Assay." Clinical Biochemistry, vol. 42, no. 1-2, 2009, pp. 83-90.
Kuhn J, Prante C, Kleesiek K, et al. Measurement of mycophenolic acid and its glucuronide using a novel rapid liquid chromatography-electrospray ionization tandem mass spectrometry assay. Clin Biochem. 2009;42(1-2):83-90.
Kuhn, J., Prante, C., Kleesiek, K., & Götting, C. (2009). Measurement of mycophenolic acid and its glucuronide using a novel rapid liquid chromatography-electrospray ionization tandem mass spectrometry assay. Clinical Biochemistry, 42(1-2), 83-90. https://doi.org/10.1016/j.clinbiochem.2008.10.004
Kuhn J, et al. Measurement of Mycophenolic Acid and Its Glucuronide Using a Novel Rapid Liquid Chromatography-electrospray Ionization Tandem Mass Spectrometry Assay. Clin Biochem. 2009;42(1-2):83-90. PubMed PMID: 18996104.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Measurement of mycophenolic acid and its glucuronide using a novel rapid liquid chromatography-electrospray ionization tandem mass spectrometry assay. AU - Kuhn,Joachim, AU - Prante,Christian, AU - Kleesiek,Knut, AU - Götting,Christian, Y1 - 2008/10/25/ PY - 2008/05/15/received PY - 2008/09/30/revised PY - 2008/10/06/accepted PY - 2008/11/11/pubmed PY - 2009/4/21/medline PY - 2008/11/11/entrez SP - 83 EP - 90 JF - Clinical biochemistry JO - Clin. Biochem. VL - 42 IS - 1-2 N2 - OBJECTIVES: Mycophenolic acid (MPA), the active metabolite of the ester prodrug mycophenolate mofetil is an immunosuppressant which selectively inhibits inosine-monophosphate dehydrogenase. The requirement for therapeutic drug monitoring shown in previous studies raises the necessity of acquiring accurate and sensitive methods to measure MPA and also its metabolite mycophenolic acid glucuronide (MPAG). DESIGN AND METHODS: We developed a robust, rapid, sensitive and highly specific HPLC-electrospray ionization mass spectrometry method to assay MPA and its metabolite MPAG in human plasma and serum. Ion suppression was investigated by a post column infusion experiment. RESULTS: Determination of MPA and MPAG were performed during a 3.0-min run time. Multiple calibration curves for the analysis of MPA and MPAG exhibited consistent linearity and reproducibility in the range of 0.05 to 100 mg/L (r>0.999) and 6 to 400 mg/L r>0.998, respectively. Limits of detection were 0.009 mg/L for MPA and 4.5 mg/L for MPAG and lower limits of quantification were 0.011 mg/L for MPA and 4.9 mg/L for MPAG. Interassay imprecision was <6.0% for both substances. Mean recovery was 48.9% (range 43.3-60.0%) for MPA and 112.2% (range 95.0-127.0%) for MPAG. Agreement was relatively good for MPA (n=122) between the presented method and a validated ELISA method (Viva analyzer, Siemens Medicals Solutions Diagnostics, NY). The Passing-Bablok regression line was: EMIT=0.91 (LC-MS/MS)+0.17 [mg/L]; r=0.97. CONCLUSIONS: This simple, robust and interference-free LC-MS/MS assay allows the rapid and accurate determination of MPA and MPAG in human plasma and other body fluids. SN - 1873-2933 UR - https://www.unboundmedicine.com/medline/citation/18996104/Measurement_of_mycophenolic_acid_and_its_glucuronide_using_a_novel_rapid_liquid_chromatography_electrospray_ionization_tandem_mass_spectrometry_assay_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0009-9120(08)00544-4 DB - PRIME DP - Unbound Medicine ER -