Tags

Type your tag names separated by a space and hit enter

The Pf3 coat protein contacts TM1 and TM3 of YidC during membrane biogenesis.
FEBS Lett 2008; 582(29):3967-72FL

Abstract

The coat protein of bacteriophage Pf3 is inserted into the plasma membrane of Escherichia coli by the insertase YidC. To identify which of the six transmembrane regions of YidC bind the single-spanning Pf3 coat protein during membrane protein biogenesis, we used the disulfide cross-linking approach. We generated single cysteines in each of the transmembrane regions of YidC and in the center of the hydrophobic region of Pf3 coat protein. We found that the substrate Pf3 coat contacts the first and third transmembrane segment (TM) of YidC as crosslinks between these two proteins can be formed in vivo during membrane biogenesis. A detailed disulfide-mapping study revealed that one face of TM3 of YidC makes contact with the Pf3 protein.

Authors+Show Affiliations

Institute of Microbiology and Molecular Biology, University of Hohenheim, 70599 Stuttgart, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18996118

Citation

Klenner, Christian, et al. "The Pf3 Coat Protein Contacts TM1 and TM3 of YidC During Membrane Biogenesis." FEBS Letters, vol. 582, no. 29, 2008, pp. 3967-72.
Klenner C, Yuan J, Dalbey RE, et al. The Pf3 coat protein contacts TM1 and TM3 of YidC during membrane biogenesis. FEBS Lett. 2008;582(29):3967-72.
Klenner, C., Yuan, J., Dalbey, R. E., & Kuhn, A. (2008). The Pf3 coat protein contacts TM1 and TM3 of YidC during membrane biogenesis. FEBS Letters, 582(29), pp. 3967-72. doi:10.1016/j.febslet.2008.10.044.
Klenner C, et al. The Pf3 Coat Protein Contacts TM1 and TM3 of YidC During Membrane Biogenesis. FEBS Lett. 2008 Dec 10;582(29):3967-72. PubMed PMID: 18996118.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Pf3 coat protein contacts TM1 and TM3 of YidC during membrane biogenesis. AU - Klenner,Christian, AU - Yuan,Jijun, AU - Dalbey,Ross E, AU - Kuhn,Andreas, Y1 - 2008/11/06/ PY - 2008/09/19/received PY - 2008/10/08/revised PY - 2008/10/10/accepted PY - 2008/11/11/pubmed PY - 2009/2/20/medline PY - 2008/11/11/entrez SP - 3967 EP - 72 JF - FEBS letters JO - FEBS Lett. VL - 582 IS - 29 N2 - The coat protein of bacteriophage Pf3 is inserted into the plasma membrane of Escherichia coli by the insertase YidC. To identify which of the six transmembrane regions of YidC bind the single-spanning Pf3 coat protein during membrane protein biogenesis, we used the disulfide cross-linking approach. We generated single cysteines in each of the transmembrane regions of YidC and in the center of the hydrophobic region of Pf3 coat protein. We found that the substrate Pf3 coat contacts the first and third transmembrane segment (TM) of YidC as crosslinks between these two proteins can be formed in vivo during membrane biogenesis. A detailed disulfide-mapping study revealed that one face of TM3 of YidC makes contact with the Pf3 protein. SN - 0014-5793 UR - https://www.unboundmedicine.com/medline/citation/18996118/The_Pf3_coat_protein_contacts_TM1_and_TM3_of_YidC_during_membrane_biogenesis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-5793(08)00876-4 DB - PRIME DP - Unbound Medicine ER -