Tags

Type your tag names separated by a space and hit enter

Frequencies of human influenza-specific antibody secreting cells or plasmablasts post vaccination from fresh and frozen peripheral blood mononuclear cells.
J Immunol Methods 2009; 340(1):42-7JI

Abstract

The rise in influenza-specific neutralizing antibody levels is proceeded by a burst of antigen-specific antibody secreting cells (ASC) or plasmablasts identified in peripheral blood approximately 5-10 days post immunization. Blood antigen-specific ASC may function as an immune marker of vaccine responses in comparison to the pre- and post-neutralizing titers; however, some have questioned whether there is adequate survival of ASC isolated from peripheral blood after freezing, making multi-center vaccine trials difficult. Here, we demonstrate similar frequencies of influenza-specific ASC from fresh and frozen peripheral blood mononuclear cells (PBMC). Influenza Hemagglutinin (HA) H1, H3, and H7-specific ASC IgG ELISpots frequencies were compared from the same fresh and frozen PBMC 7 days after 2006 Trivalent Influenza Vaccine (TIV) in 10 young healthy subjects. H1-, H3-, and H7-specific IgG ASC spots/10(6) from fresh PBMC on day 7 were 229+/-341, 98+/-90, and 6+/-11 respectively. Total IgG ASC spots/million PBMC pre- and 7-day post-vaccination were 290+/-188 (0.029% PBMC) and 1691+/-836 (0.17% PBMC) respectively. There was no difference in the H1 -H3-, and total specific ASC IgG ELISpot frequencies from the fresh versus frozen PBMC on day 7 (p=0.43, 0.28, 0.28 respectively). These results demonstrate feasibility of testing whether antigen-specific ASC from frozen PBMC are an early biomarker of long-term antibody responses in multi-center vaccine trials.

Authors+Show Affiliations

Division of Pulmonary & Critical Care Medicine, NY 14642, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18996127

Citation

Kyu, Shuya Y., et al. "Frequencies of Human Influenza-specific Antibody Secreting Cells or Plasmablasts Post Vaccination From Fresh and Frozen Peripheral Blood Mononuclear Cells." Journal of Immunological Methods, vol. 340, no. 1, 2009, pp. 42-7.
Kyu SY, Kobie J, Yang H, et al. Frequencies of human influenza-specific antibody secreting cells or plasmablasts post vaccination from fresh and frozen peripheral blood mononuclear cells. J Immunol Methods. 2009;340(1):42-7.
Kyu, S. Y., Kobie, J., Yang, H., Zand, M. S., Topham, D. J., Quataert, S. A., ... Lee, F. E. (2009). Frequencies of human influenza-specific antibody secreting cells or plasmablasts post vaccination from fresh and frozen peripheral blood mononuclear cells. Journal of Immunological Methods, 340(1), pp. 42-7. doi:10.1016/j.jim.2008.09.025.
Kyu SY, et al. Frequencies of Human Influenza-specific Antibody Secreting Cells or Plasmablasts Post Vaccination From Fresh and Frozen Peripheral Blood Mononuclear Cells. J Immunol Methods. 2009 Jan 1;340(1):42-7. PubMed PMID: 18996127.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Frequencies of human influenza-specific antibody secreting cells or plasmablasts post vaccination from fresh and frozen peripheral blood mononuclear cells. AU - Kyu,Shuya Y, AU - Kobie,James, AU - Yang,Hongmei, AU - Zand,Martin S, AU - Topham,David J, AU - Quataert,Sally A, AU - Sanz,Ignacio, AU - Lee,F Eun-Hyung, Y1 - 2008/11/06/ PY - 2008/04/24/received PY - 2008/09/26/revised PY - 2008/09/30/accepted PY - 2008/11/11/entrez PY - 2008/11/11/pubmed PY - 2009/3/6/medline SP - 42 EP - 7 JF - Journal of immunological methods JO - J. Immunol. Methods VL - 340 IS - 1 N2 - The rise in influenza-specific neutralizing antibody levels is proceeded by a burst of antigen-specific antibody secreting cells (ASC) or plasmablasts identified in peripheral blood approximately 5-10 days post immunization. Blood antigen-specific ASC may function as an immune marker of vaccine responses in comparison to the pre- and post-neutralizing titers; however, some have questioned whether there is adequate survival of ASC isolated from peripheral blood after freezing, making multi-center vaccine trials difficult. Here, we demonstrate similar frequencies of influenza-specific ASC from fresh and frozen peripheral blood mononuclear cells (PBMC). Influenza Hemagglutinin (HA) H1, H3, and H7-specific ASC IgG ELISpots frequencies were compared from the same fresh and frozen PBMC 7 days after 2006 Trivalent Influenza Vaccine (TIV) in 10 young healthy subjects. H1-, H3-, and H7-specific IgG ASC spots/10(6) from fresh PBMC on day 7 were 229+/-341, 98+/-90, and 6+/-11 respectively. Total IgG ASC spots/million PBMC pre- and 7-day post-vaccination were 290+/-188 (0.029% PBMC) and 1691+/-836 (0.17% PBMC) respectively. There was no difference in the H1 -H3-, and total specific ASC IgG ELISpot frequencies from the fresh versus frozen PBMC on day 7 (p=0.43, 0.28, 0.28 respectively). These results demonstrate feasibility of testing whether antigen-specific ASC from frozen PBMC are an early biomarker of long-term antibody responses in multi-center vaccine trials. SN - 0022-1759 UR - https://www.unboundmedicine.com/medline/citation/18996127/Frequencies_of_human_influenza_specific_antibody_secreting_cells_or_plasmablasts_post_vaccination_from_fresh_and_frozen_peripheral_blood_mononuclear_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-1759(08)00309-8 DB - PRIME DP - Unbound Medicine ER -