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Investigations of the phytochemical content of Sceletium tortuosum following the preparation of "Kougoed" by fermentation of plant material.
J Ethnopharmacol. 2009 Jan 12; 121(1):86-91.JE

Abstract

AIM OF THE STUDY

Sceletium plant species that contain alkaloids are claimed to have mood elevation and anti-anxiety properties, especially after the plant material has been fermented. The fermented preparation is locally known as "kougoed" or "channa" and has been emphasized and advertised for its increased potency when incorporated in commercial products. The aim of the study was to investigate quantitative and qualitative changes in alkaloidal content following fermentation of plant samples carried out under controlled conditions and also on pure mesembrine hydrochloride (MHCl).

MATERIALS AND METHODS

Samples were prepared from the aerial parts of Sceletium tortuosum. Studies were also conducted on mesembrine hydrochloride (MHCl) in aqueous and methanolic solutions under similar conditions of exposure to sunlight as well as under ambient and elevated temperature (40+/-2 degrees C). Quantitative and qualitative changes in alkaloidal content were monitored by HPLC and LC-MS, respectively.

RESULTS AND CONCLUSIONS

The initial fermentation study showed transformation of mesembrine to Delta(7)mesembrenone, where the content of the former decreased from a concentration of 1.33% to 0.05% whilst the latter increased from below its limit of quantitation (LoQ) to 0.11% on the 10th day. The experiments on pure MHCl revealed similar transformations in aqueous solutions whereas no change was seen in methanolic solutions. Sunlight and aqueous conditions appear necessary to facilitate the transformation, which was confirmed by the absence of such a transformation when solutions of MHCl were kept in the dark.

Authors+Show Affiliations

Division of Pharmaceutics, Faculty of Pharmacy, Rhodes University, Artillery Road, Grahamstown, East Cape, South Africa.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18996176

Citation

Patnala, Srinivas, and Isadore Kanfer. "Investigations of the Phytochemical Content of Sceletium Tortuosum Following the Preparation of "Kougoed" By Fermentation of Plant Material." Journal of Ethnopharmacology, vol. 121, no. 1, 2009, pp. 86-91.
Patnala S, Kanfer I. Investigations of the phytochemical content of Sceletium tortuosum following the preparation of "Kougoed" by fermentation of plant material. J Ethnopharmacol. 2009;121(1):86-91.
Patnala, S., & Kanfer, I. (2009). Investigations of the phytochemical content of Sceletium tortuosum following the preparation of "Kougoed" by fermentation of plant material. Journal of Ethnopharmacology, 121(1), 86-91. https://doi.org/10.1016/j.jep.2008.10.008
Patnala S, Kanfer I. Investigations of the Phytochemical Content of Sceletium Tortuosum Following the Preparation of "Kougoed" By Fermentation of Plant Material. J Ethnopharmacol. 2009 Jan 12;121(1):86-91. PubMed PMID: 18996176.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigations of the phytochemical content of Sceletium tortuosum following the preparation of "Kougoed" by fermentation of plant material. AU - Patnala,Srinivas, AU - Kanfer,Isadore, Y1 - 2008/10/17/ PY - 2008/05/05/received PY - 2008/08/05/revised PY - 2008/10/05/accepted PY - 2008/11/11/pubmed PY - 2009/4/1/medline PY - 2008/11/11/entrez SP - 86 EP - 91 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 121 IS - 1 N2 - AIM OF THE STUDY: Sceletium plant species that contain alkaloids are claimed to have mood elevation and anti-anxiety properties, especially after the plant material has been fermented. The fermented preparation is locally known as "kougoed" or "channa" and has been emphasized and advertised for its increased potency when incorporated in commercial products. The aim of the study was to investigate quantitative and qualitative changes in alkaloidal content following fermentation of plant samples carried out under controlled conditions and also on pure mesembrine hydrochloride (MHCl). MATERIALS AND METHODS: Samples were prepared from the aerial parts of Sceletium tortuosum. Studies were also conducted on mesembrine hydrochloride (MHCl) in aqueous and methanolic solutions under similar conditions of exposure to sunlight as well as under ambient and elevated temperature (40+/-2 degrees C). Quantitative and qualitative changes in alkaloidal content were monitored by HPLC and LC-MS, respectively. RESULTS AND CONCLUSIONS: The initial fermentation study showed transformation of mesembrine to Delta(7)mesembrenone, where the content of the former decreased from a concentration of 1.33% to 0.05% whilst the latter increased from below its limit of quantitation (LoQ) to 0.11% on the 10th day. The experiments on pure MHCl revealed similar transformations in aqueous solutions whereas no change was seen in methanolic solutions. Sunlight and aqueous conditions appear necessary to facilitate the transformation, which was confirmed by the absence of such a transformation when solutions of MHCl were kept in the dark. SN - 0378-8741 UR - https://www.unboundmedicine.com/medline/citation/18996176/Investigations_of_the_phytochemical_content_of_Sceletium_tortuosum_following_the_preparation_of_"Kougoed"_by_fermentation_of_plant_material_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(08)00559-X DB - PRIME DP - Unbound Medicine ER -