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Propofol limits rat myocardial ischemia and reperfusion injury with an associated reduction in apoptotic cell death in vivo.
Vascul Pharmacol. 2009 Jan-Feb; 50(1-2):71-7.VP

Abstract

Propofol, a rapidly acting, short duration, intravenous hypnotic anesthetic induction agent, is often used in clinical situations where myocardial ischemia/ reperfusion (I/R) injury is a threat. The aim of the present study was to evaluate the protective effect of propofol on myocardial I/R injury in rat due to apoptosis. Myocardial I/R injury were induced by occluding the left anterior descending (LAD) coronary artery for 25 min followed by either 2 h or 6 h reperfusion. Apoptosis was evaluated by Western blot analysis (Bcl-2, Bax expression), DNA strand breaks, TUNEL analysis and measuring myocardial caspase-3 activity. Propofol significantly reduced infarct size and improved I/R-induced myocardial contractile dysfunction by improving left ventricular diastolic pressure and positive and negative maximal values of the first derivative (+dp/dt) of left ventricular pressure. Propofol increased Bcl-2/Bax expression ratio and decreased caspase-3 activity in I/R rat hearts, which resulted in reduction of myocardial apoptosis as evidenced by TUNEL analysis and DNA laddering experiments. In an in vitro study, propofol increased H9c2 cell viability against oxidative stress induced by glucose oxidase (GOX) in a dose-dependent manner. These data suggest propofol limits I/R injury with an associated reduction in apoptotic cell death in vivo.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Jin YC
Department of Pharmacology School of Medicine and Institute of Health Sciences, Gyeongsang National University, Jinju, Republic of Korea.
Kim W
No affiliation info available
Ha YM
No affiliation info available
Shin IW
No affiliation info available
Sohn JT
No affiliation info available
Kim HJ
No affiliation info available
Seo HG
No affiliation info available
Lee JH
No affiliation info available
Chang KC
No affiliation info available

MeSH

Anesthetics, IntravenousAnimalsApoptosisCaspase 3Hypnotics and SedativesIn Situ Nick-End LabelingMaleMyocardial ContractionMyocardial Reperfusion InjuryMyocytes, CardiacPropofolRatsRats, Sprague-Dawleybcl-2-Associated X Protein

Pub Type(s)

Journal Article

Language

eng

PubMed ID

18996224

Citation

Jin, Yong Chun, et al. "Propofol Limits Rat Myocardial Ischemia and Reperfusion Injury With an Associated Reduction in Apoptotic Cell Death in Vivo." Vascular Pharmacology, vol. 50, no. 1-2, 2009, pp. 71-7.
Jin YC, Kim W, Ha YM, et al. Propofol limits rat myocardial ischemia and reperfusion injury with an associated reduction in apoptotic cell death in vivo. Vascul Pharmacol. 2009;50(1-2):71-7.
Jin, Y. C., Kim, W., Ha, Y. M., Shin, I. W., Sohn, J. T., Kim, H. J., Seo, H. G., Lee, J. H., & Chang, K. C. (2009). Propofol limits rat myocardial ischemia and reperfusion injury with an associated reduction in apoptotic cell death in vivo. Vascular Pharmacology, 50(1-2), 71-7. https://doi.org/10.1016/j.vph.2008.10.002
Jin YC, et al. Propofol Limits Rat Myocardial Ischemia and Reperfusion Injury With an Associated Reduction in Apoptotic Cell Death in Vivo. Vascul Pharmacol. 2009 Jan-Feb;50(1-2):71-7. PubMed PMID: 18996224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Propofol limits rat myocardial ischemia and reperfusion injury with an associated reduction in apoptotic cell death in vivo. AU - Jin,Yong Chun, AU - Kim,Wooyeol, AU - Ha,Yu Mi, AU - Shin,Il Woo, AU - Sohn,Ju Tae, AU - Kim,Hye Jung, AU - Seo,Han Geuk, AU - Lee,Jea Heun, AU - Chang,Ki Churl, Y1 - 2008/10/28/ PY - 2008/05/26/received PY - 2008/08/20/revised PY - 2008/10/13/accepted PY - 2008/11/11/entrez PY - 2008/11/11/pubmed PY - 2009/4/28/medline SP - 71 EP - 7 JF - Vascular pharmacology JO - Vascul Pharmacol VL - 50 IS - 1-2 N2 - Propofol, a rapidly acting, short duration, intravenous hypnotic anesthetic induction agent, is often used in clinical situations where myocardial ischemia/ reperfusion (I/R) injury is a threat. The aim of the present study was to evaluate the protective effect of propofol on myocardial I/R injury in rat due to apoptosis. Myocardial I/R injury were induced by occluding the left anterior descending (LAD) coronary artery for 25 min followed by either 2 h or 6 h reperfusion. Apoptosis was evaluated by Western blot analysis (Bcl-2, Bax expression), DNA strand breaks, TUNEL analysis and measuring myocardial caspase-3 activity. Propofol significantly reduced infarct size and improved I/R-induced myocardial contractile dysfunction by improving left ventricular diastolic pressure and positive and negative maximal values of the first derivative (+dp/dt) of left ventricular pressure. Propofol increased Bcl-2/Bax expression ratio and decreased caspase-3 activity in I/R rat hearts, which resulted in reduction of myocardial apoptosis as evidenced by TUNEL analysis and DNA laddering experiments. In an in vitro study, propofol increased H9c2 cell viability against oxidative stress induced by glucose oxidase (GOX) in a dose-dependent manner. These data suggest propofol limits I/R injury with an associated reduction in apoptotic cell death in vivo. SN - 1537-1891 UR - https://www.unboundmedicine.com/medline/citation/18996224/Propofol_limits_rat_myocardial_ischemia_and_reperfusion_injury_with_an_associated_reduction_in_apoptotic_cell_death_in_vivo_ DB - PRIME DP - Unbound Medicine ER -