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Determination of estrogenic compounds in wastewater using liquid chromatography-tandem mass spectrometry with electrospray and atmospheric pressure photoionization following desalting extraction.
Chemosphere. 2009 Jan; 74(4):508-14.C

Abstract

Two complementary LC-MS ionization methods, electrospray (ESI) and atmospheric pressure photoionization (APPI), have been optimized to determine three natural estrogenic compounds (estrone, 17beta-estradiol and estriol) and two synthetic estrogenic compounds (17alpha-ethynylestradiol and diethylstilbestrol) in the influent and effluent of wastewater treatment plants (WWTPs). The wastewater samples were first subjected to solid-phase extraction coupled with desalting extraction to remove matrix interference. The analytes were then detected using liquid chromatography-tandem mass spectrometry (LC-MS-MS) with ESI and dopant-assisted (DA) APPI to evaluate the ion suppression effect and to complement the detection and quantification of estrogenic compounds in complex wastewater samples. The average ion suppression factors for the extracts of the WWTP influent analyzed using ESI and APPI were 52+/-5% and 27+/-7%, respectively. The sensitivity and ionization efficiency of the LC-ESI-MS-MS system decreased dramatically when a complex matrix was present in the WWTP influent sample. Estrogenic compounds could be detected in the WWTP influent and effluent samples at concentrations below the parts-per-billion level. The lower detection limits obtained when using ESI and the higher matrix tolerance of the APPI method allowed the complete quantification of estrogenic compounds in very complex samples in a complementary manner.

Authors+Show Affiliations

Department of Chemistry, National Central University, Chung-Li 32054, Taiwan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18996561

Citation

Chen, Hsin-Chang, et al. "Determination of Estrogenic Compounds in Wastewater Using Liquid Chromatography-tandem Mass Spectrometry With Electrospray and Atmospheric Pressure Photoionization Following Desalting Extraction." Chemosphere, vol. 74, no. 4, 2009, pp. 508-14.
Chen HC, Kuo HW, Ding WH. Determination of estrogenic compounds in wastewater using liquid chromatography-tandem mass spectrometry with electrospray and atmospheric pressure photoionization following desalting extraction. Chemosphere. 2009;74(4):508-14.
Chen, H. C., Kuo, H. W., & Ding, W. H. (2009). Determination of estrogenic compounds in wastewater using liquid chromatography-tandem mass spectrometry with electrospray and atmospheric pressure photoionization following desalting extraction. Chemosphere, 74(4), 508-14. https://doi.org/10.1016/j.chemosphere.2008.09.089
Chen HC, Kuo HW, Ding WH. Determination of Estrogenic Compounds in Wastewater Using Liquid Chromatography-tandem Mass Spectrometry With Electrospray and Atmospheric Pressure Photoionization Following Desalting Extraction. Chemosphere. 2009;74(4):508-14. PubMed PMID: 18996561.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of estrogenic compounds in wastewater using liquid chromatography-tandem mass spectrometry with electrospray and atmospheric pressure photoionization following desalting extraction. AU - Chen,Hsin-Chang, AU - Kuo,Han-Wen, AU - Ding,Wang-Hsien, Y1 - 2008/11/08/ PY - 2008/06/06/received PY - 2008/09/27/revised PY - 2008/09/29/accepted PY - 2008/11/11/pubmed PY - 2009/2/20/medline PY - 2008/11/11/entrez SP - 508 EP - 14 JF - Chemosphere JO - Chemosphere VL - 74 IS - 4 N2 - Two complementary LC-MS ionization methods, electrospray (ESI) and atmospheric pressure photoionization (APPI), have been optimized to determine three natural estrogenic compounds (estrone, 17beta-estradiol and estriol) and two synthetic estrogenic compounds (17alpha-ethynylestradiol and diethylstilbestrol) in the influent and effluent of wastewater treatment plants (WWTPs). The wastewater samples were first subjected to solid-phase extraction coupled with desalting extraction to remove matrix interference. The analytes were then detected using liquid chromatography-tandem mass spectrometry (LC-MS-MS) with ESI and dopant-assisted (DA) APPI to evaluate the ion suppression effect and to complement the detection and quantification of estrogenic compounds in complex wastewater samples. The average ion suppression factors for the extracts of the WWTP influent analyzed using ESI and APPI were 52+/-5% and 27+/-7%, respectively. The sensitivity and ionization efficiency of the LC-ESI-MS-MS system decreased dramatically when a complex matrix was present in the WWTP influent sample. Estrogenic compounds could be detected in the WWTP influent and effluent samples at concentrations below the parts-per-billion level. The lower detection limits obtained when using ESI and the higher matrix tolerance of the APPI method allowed the complete quantification of estrogenic compounds in very complex samples in a complementary manner. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/18996561/Determination_of_estrogenic_compounds_in_wastewater_using_liquid_chromatography_tandem_mass_spectrometry_with_electrospray_and_atmospheric_pressure_photoionization_following_desalting_extraction_ DB - PRIME DP - Unbound Medicine ER -