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Soybean (Glycine max) allergy in Europe: Gly m 5 (beta-conglycinin) and Gly m 6 (glycinin) are potential diagnostic markers for severe allergic reactions to soy.
J Allergy Clin Immunol 2009; 123(2):452-8JA

Abstract

BACKGROUND

Soybean is considered an important allergenic food, but published data on soybean allergens are controversial.

OBJECTIVE

We sought to identify relevant soybean allergens and correlate the IgE-binding pattern to clinical characteristics in European patients with confirmed soy allergy.

METHODS

IgE-reactive proteins were identified from a soybean cDNA expression library, purified from natural soybean source, or expressed in Escherichia coli. The IgE reactivity in 30 sera from subjects with a positive double-blind, placebo-controlled soybean challenge (n = 25) or a convincing history of anaphylaxis to soy (n = 5) was analyzed by ELISA or CAP-FEIA.

RESULTS

All subunits of Gly m 5 (beta-conglycinin) and Gly m 6 (glycinin) were IgE-reactive: 53% (16/30) of the study subjects had specific IgE to at least 1 major storage protein, 43% (13/30) to Gly m 5 , and 36% (11/30) to Gly m 6. Gly m 5 was IgE-reactive in 5 of 5 and Gly m 6 in 3 of 5 children. IgE-binding to Gly m 5 or Gly m 6 was found in 86% (6/7) subjects with anaphylaxis to soy and in 55% (6/11) of subjects with moderate but only 33% (4/12) of subjects with mild soy-related symptoms. The odds ratio (P < .05) for severe versus mild allergic reactions in subjects with specific IgE to Gly m 5 or Gly m6 was 12/1.

CONCLUSION

Sensitization to the soybean allergens Gly m 5 or Gly m 6 is potentially indicative for severe allergic reactions to soy.

Authors+Show Affiliations

Division of Allergology, Paul-Ehrlich-Institut, Langen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18996574

Citation

Holzhauser, Thomas, et al. "Soybean (Glycine Max) Allergy in Europe: Gly M 5 (beta-conglycinin) and Gly M 6 (glycinin) Are Potential Diagnostic Markers for Severe Allergic Reactions to Soy." The Journal of Allergy and Clinical Immunology, vol. 123, no. 2, 2009, pp. 452-8.
Holzhauser T, Wackermann O, Ballmer-Weber BK, et al. Soybean (Glycine max) allergy in Europe: Gly m 5 (beta-conglycinin) and Gly m 6 (glycinin) are potential diagnostic markers for severe allergic reactions to soy. J Allergy Clin Immunol. 2009;123(2):452-8.
Holzhauser, T., Wackermann, O., Ballmer-Weber, B. K., Bindslev-Jensen, C., Scibilia, J., Perono-Garoffo, L., ... Vieths, S. (2009). Soybean (Glycine max) allergy in Europe: Gly m 5 (beta-conglycinin) and Gly m 6 (glycinin) are potential diagnostic markers for severe allergic reactions to soy. The Journal of Allergy and Clinical Immunology, 123(2), pp. 452-8. doi:10.1016/j.jaci.2008.09.034.
Holzhauser T, et al. Soybean (Glycine Max) Allergy in Europe: Gly M 5 (beta-conglycinin) and Gly M 6 (glycinin) Are Potential Diagnostic Markers for Severe Allergic Reactions to Soy. J Allergy Clin Immunol. 2009;123(2):452-8. PubMed PMID: 18996574.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Soybean (Glycine max) allergy in Europe: Gly m 5 (beta-conglycinin) and Gly m 6 (glycinin) are potential diagnostic markers for severe allergic reactions to soy. AU - Holzhauser,Thomas, AU - Wackermann,Olga, AU - Ballmer-Weber,Barbara K, AU - Bindslev-Jensen,Carsten, AU - Scibilia,Joseph, AU - Perono-Garoffo,Lorenza, AU - Utsumi,Shigeru, AU - Poulsen,Lars K, AU - Vieths,Stefan, Y1 - 2008/11/08/ PY - 2008/06/04/received PY - 2008/09/10/revised PY - 2008/09/19/accepted PY - 2008/11/11/pubmed PY - 2009/3/4/medline PY - 2008/11/11/entrez SP - 452 EP - 8 JF - The Journal of allergy and clinical immunology JO - J. Allergy Clin. Immunol. VL - 123 IS - 2 N2 - BACKGROUND: Soybean is considered an important allergenic food, but published data on soybean allergens are controversial. OBJECTIVE: We sought to identify relevant soybean allergens and correlate the IgE-binding pattern to clinical characteristics in European patients with confirmed soy allergy. METHODS: IgE-reactive proteins were identified from a soybean cDNA expression library, purified from natural soybean source, or expressed in Escherichia coli. The IgE reactivity in 30 sera from subjects with a positive double-blind, placebo-controlled soybean challenge (n = 25) or a convincing history of anaphylaxis to soy (n = 5) was analyzed by ELISA or CAP-FEIA. RESULTS: All subunits of Gly m 5 (beta-conglycinin) and Gly m 6 (glycinin) were IgE-reactive: 53% (16/30) of the study subjects had specific IgE to at least 1 major storage protein, 43% (13/30) to Gly m 5 , and 36% (11/30) to Gly m 6. Gly m 5 was IgE-reactive in 5 of 5 and Gly m 6 in 3 of 5 children. IgE-binding to Gly m 5 or Gly m 6 was found in 86% (6/7) subjects with anaphylaxis to soy and in 55% (6/11) of subjects with moderate but only 33% (4/12) of subjects with mild soy-related symptoms. The odds ratio (P < .05) for severe versus mild allergic reactions in subjects with specific IgE to Gly m 5 or Gly m6 was 12/1. CONCLUSION: Sensitization to the soybean allergens Gly m 5 or Gly m 6 is potentially indicative for severe allergic reactions to soy. SN - 1097-6825 UR - https://www.unboundmedicine.com/medline/citation/18996574/Soybean__Glycine_max__allergy_in_Europe:_Gly_m_5__beta_conglycinin__and_Gly_m_6__glycinin__are_potential_diagnostic_markers_for_severe_allergic_reactions_to_soy_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0091-6749(08)01729-6 DB - PRIME DP - Unbound Medicine ER -