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Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45gamma.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 01; 64(Pt 11):1070-3.AC

Abstract

Gadd45, MyD118 and CR6 (also termed Gadd45alpha, Gadd45beta and Gadd45gamma, respectively) comprise a family of proteins that play important roles in negative growth control, maintenance of genomic stability, DNA repair, cell-cycle control and apoptosis. Recombinant human Gadd45gamma and its selenomethionine derivative were expressed in an Escherichia coli expression system and purified; they were then crystallized using the hanging-drop vapour-diffusion method. Diffraction-quality crystals were grown at 291 K using PEG 3350 as precipitant. Using synchrotron radiation, the best diffraction data were collected to 2.3 A resolution for native crystals at 100 K; selenomethionyl derivative data were collected to 3.3 A resolution. All the crystals belonged to space group I2(1)3, with approximate unit-cell parameters a = b = c = 126 A.

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Authors+Show Affiliations

Zhang W
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.
Zhao M
No affiliation info available
Li J
No affiliation info available
Li X
No affiliation info available
Zeng Z
No affiliation info available
Rao Z
No affiliation info available

MeSH

CrystallizationHumansIntracellular Signaling Peptides and ProteinsMolecular Sequence DataRecombinant ProteinsSelenomethionineSynchrotronsX-Ray Diffraction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18997345

Citation

Zhang, Wenzheng, et al. "Purification, Crystallization and Preliminary X-ray Diffraction Analysis of Human Gadd45gamma." Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, vol. 64, no. Pt 11, 2008, pp. 1070-3.
Zhang W, Zhao M, Li J, et al. Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45gamma. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008;64(Pt 11):1070-3.
Zhang, W., Zhao, M., Li, J., Li, X., Zeng, Z., & Rao, Z. (2008). Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45gamma. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 64(Pt 11), 1070-3. https://doi.org/10.1107/S174430910803306X
Zhang W, et al. Purification, Crystallization and Preliminary X-ray Diffraction Analysis of Human Gadd45gamma. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 1;64(Pt 11):1070-3. PubMed PMID: 18997345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45gamma. AU - Zhang,Wenzheng, AU - Zhao,Mingzhuo, AU - Li,Jianhui, AU - Li,Xuemei, AU - Zeng,ZongHao, AU - Rao,Zihe, Y1 - 2008/10/31/ PY - 2008/06/25/received PY - 2008/10/13/accepted PY - 2008/11/11/pubmed PY - 2008/12/17/medline PY - 2008/11/11/entrez SP - 1070 EP - 3 JF - Acta crystallographica. Section F, Structural biology and crystallization communications JO - Acta Crystallogr Sect F Struct Biol Cryst Commun VL - 64 IS - Pt 11 N2 - Gadd45, MyD118 and CR6 (also termed Gadd45alpha, Gadd45beta and Gadd45gamma, respectively) comprise a family of proteins that play important roles in negative growth control, maintenance of genomic stability, DNA repair, cell-cycle control and apoptosis. Recombinant human Gadd45gamma and its selenomethionine derivative were expressed in an Escherichia coli expression system and purified; they were then crystallized using the hanging-drop vapour-diffusion method. Diffraction-quality crystals were grown at 291 K using PEG 3350 as precipitant. Using synchrotron radiation, the best diffraction data were collected to 2.3 A resolution for native crystals at 100 K; selenomethionyl derivative data were collected to 3.3 A resolution. All the crystals belonged to space group I2(1)3, with approximate unit-cell parameters a = b = c = 126 A. SN - 1744-3091 UR - https://www.unboundmedicine.com/medline/citation/18997345/Purification_crystallization_and_preliminary_X_ray_diffraction_analysis_of_human_Gadd45gamma_ L2 - http://scripts.iucr.org/cgi-bin/paper?S174430910803306X DB - PRIME DP - Unbound Medicine ER -