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Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45gamma.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 01; 64(Pt 11):1070-3.AC

Abstract

Gadd45, MyD118 and CR6 (also termed Gadd45alpha, Gadd45beta and Gadd45gamma, respectively) comprise a family of proteins that play important roles in negative growth control, maintenance of genomic stability, DNA repair, cell-cycle control and apoptosis. Recombinant human Gadd45gamma and its selenomethionine derivative were expressed in an Escherichia coli expression system and purified; they were then crystallized using the hanging-drop vapour-diffusion method. Diffraction-quality crystals were grown at 291 K using PEG 3350 as precipitant. Using synchrotron radiation, the best diffraction data were collected to 2.3 A resolution for native crystals at 100 K; selenomethionyl derivative data were collected to 3.3 A resolution. All the crystals belonged to space group I2(1)3, with approximate unit-cell parameters a = b = c = 126 A.

Authors+Show Affiliations

National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18997345

Citation

Zhang, Wenzheng, et al. "Purification, Crystallization and Preliminary X-ray Diffraction Analysis of Human Gadd45gamma." Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, vol. 64, no. Pt 11, 2008, pp. 1070-3.
Zhang W, Zhao M, Li J, et al. Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45gamma. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008;64(Pt 11):1070-3.
Zhang, W., Zhao, M., Li, J., Li, X., Zeng, Z., & Rao, Z. (2008). Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45gamma. Acta Crystallographica. Section F, Structural Biology and Crystallization Communications, 64(Pt 11), 1070-3. https://doi.org/10.1107/S174430910803306X
Zhang W, et al. Purification, Crystallization and Preliminary X-ray Diffraction Analysis of Human Gadd45gamma. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 1;64(Pt 11):1070-3. PubMed PMID: 18997345.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45gamma. AU - Zhang,Wenzheng, AU - Zhao,Mingzhuo, AU - Li,Jianhui, AU - Li,Xuemei, AU - Zeng,ZongHao, AU - Rao,Zihe, Y1 - 2008/10/31/ PY - 2008/06/25/received PY - 2008/10/13/accepted PY - 2008/11/11/pubmed PY - 2008/12/17/medline PY - 2008/11/11/entrez SP - 1070 EP - 3 JF - Acta crystallographica. Section F, Structural biology and crystallization communications JO - Acta Crystallogr Sect F Struct Biol Cryst Commun VL - 64 IS - Pt 11 N2 - Gadd45, MyD118 and CR6 (also termed Gadd45alpha, Gadd45beta and Gadd45gamma, respectively) comprise a family of proteins that play important roles in negative growth control, maintenance of genomic stability, DNA repair, cell-cycle control and apoptosis. Recombinant human Gadd45gamma and its selenomethionine derivative were expressed in an Escherichia coli expression system and purified; they were then crystallized using the hanging-drop vapour-diffusion method. Diffraction-quality crystals were grown at 291 K using PEG 3350 as precipitant. Using synchrotron radiation, the best diffraction data were collected to 2.3 A resolution for native crystals at 100 K; selenomethionyl derivative data were collected to 3.3 A resolution. All the crystals belonged to space group I2(1)3, with approximate unit-cell parameters a = b = c = 126 A. SN - 1744-3091 UR - https://www.unboundmedicine.com/medline/citation/18997345/Purification_crystallization_and_preliminary_X_ray_diffraction_analysis_of_human_Gadd45gamma_ L2 - http://scripts.iucr.org/cgi-bin/paper?S174430910803306X DB - PRIME DP - Unbound Medicine ER -