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Cloning and characterization of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes of a natural-rubber producing plant, Hevea brasiliensis.Biosci Biotechnol Biochem. 2008 Nov; 72(11):2903-17.BB
Abstract
Natural rubber is synthesized as rubber particles in the latex, the fluid cytoplasm of laticifers, of Hevea brasiliensis. Although it has been found that natural rubber is biosynthesized through the mevalonate pathway, the involvement of an alternative 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway is uncertain. We obtained all series of the MEP pathway candidate genes by analyzing expressed sequence tag (EST) information and degenerate PCR in H. brasiliensis. Complementation experiments with Escherichia coli mutants were performed to confirm the functions of the MEP pathway gene products of H. brasiliensis together with those of Arabidopsis thaliana, and it was found that 1-deoxy-D-xylulose-5-phosphate reductoisomerase, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, and 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase of H. brasiliensis were functionally active in the E. coli mutants. Gene expression analysis revealed that the expression level of the HbDXS2 gene in latex was relatively high as compared to those of other MEP pathway genes. However, a feeding experiment with [1-(13)C] 1-deoxy-D-xylulose triacetate, an intermediate derivative of the MEP pathway, indicated that the MEP pathway is not involved in rubber biosynthesis, but is involved in carotenoids biosynthesis in H. brasiliensis.
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MeSH
Pub Type(s)
Journal Article
Language
eng
PubMed ID
18997428
Citation
Sando, Tomoki, et al. "Cloning and Characterization of the 2-C-methyl-D-erythritol 4-phosphate (MEP) Pathway Genes of a Natural-rubber Producing Plant, Hevea Brasiliensis." Bioscience, Biotechnology, and Biochemistry, vol. 72, no. 11, 2008, pp. 2903-17.
Sando T, Takeno S, Watanabe N, et al. Cloning and characterization of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes of a natural-rubber producing plant, Hevea brasiliensis. Biosci Biotechnol Biochem. 2008;72(11):2903-17.
Sando, T., Takeno, S., Watanabe, N., Okumoto, H., Kuzuyama, T., Yamashita, A., Hattori, M., Ogasawara, N., Fukusaki, E., & Kobayashi, A. (2008). Cloning and characterization of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes of a natural-rubber producing plant, Hevea brasiliensis. Bioscience, Biotechnology, and Biochemistry, 72(11), 2903-17.
Sando T, et al. Cloning and Characterization of the 2-C-methyl-D-erythritol 4-phosphate (MEP) Pathway Genes of a Natural-rubber Producing Plant, Hevea Brasiliensis. Biosci Biotechnol Biochem. 2008;72(11):2903-17. PubMed PMID: 18997428.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Cloning and characterization of the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway genes of a natural-rubber producing plant, Hevea brasiliensis.
AU - Sando,Tomoki,
AU - Takeno,Shinya,
AU - Watanabe,Norie,
AU - Okumoto,Hiroshi,
AU - Kuzuyama,Tomohisa,
AU - Yamashita,Atsushi,
AU - Hattori,Masahira,
AU - Ogasawara,Naotake,
AU - Fukusaki,Eiichiro,
AU - Kobayashi,Akio,
Y1 - 2008/11/07/
PY - 2008/11/11/pubmed
PY - 2009/1/31/medline
PY - 2008/11/11/entrez
SP - 2903
EP - 17
JF - Bioscience, biotechnology, and biochemistry
JO - Biosci Biotechnol Biochem
VL - 72
IS - 11
N2 - Natural rubber is synthesized as rubber particles in the latex, the fluid cytoplasm of laticifers, of Hevea brasiliensis. Although it has been found that natural rubber is biosynthesized through the mevalonate pathway, the involvement of an alternative 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway is uncertain. We obtained all series of the MEP pathway candidate genes by analyzing expressed sequence tag (EST) information and degenerate PCR in H. brasiliensis. Complementation experiments with Escherichia coli mutants were performed to confirm the functions of the MEP pathway gene products of H. brasiliensis together with those of Arabidopsis thaliana, and it was found that 1-deoxy-D-xylulose-5-phosphate reductoisomerase, 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, and 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase of H. brasiliensis were functionally active in the E. coli mutants. Gene expression analysis revealed that the expression level of the HbDXS2 gene in latex was relatively high as compared to those of other MEP pathway genes. However, a feeding experiment with [1-(13)C] 1-deoxy-D-xylulose triacetate, an intermediate derivative of the MEP pathway, indicated that the MEP pathway is not involved in rubber biosynthesis, but is involved in carotenoids biosynthesis in H. brasiliensis.
SN - 1347-6947
UR - https://www.unboundmedicine.com/medline/citation/18997428/Cloning_and_characterization_of_the_2_C_methyl_D_erythritol_4_phosphate__MEP__pathway_genes_of_a_natural_rubber_producing_plant_Hevea_brasiliensis_
L2 - https://joi.jlc.jst.go.jp/JST.JSTAGE/bbb/80387?lang=en&from=PubMed
DB - PRIME
DP - Unbound Medicine
ER -
