Tags

Type your tag names separated by a space and hit enter

Post-reassortment amino acid change in the hemagglutinin of a human-avian influenza H5N1 reassortant virus alters its antigenic specificity.
Acta Virol. 2008; 52(3):181-4.AV

Abstract

It was shown earlier that the reassortant influenza virus having hemagglutinin (HA) gene of A/Duck/Primorie/2621/2001 (H5N2) virus and 7 genes of A/Puerto Rico/8/34 (H1N1) virus produced low yields in embryonated chicken eggs. We found that a variant reassortant selected by serial passages in eggs produced higher yields than the initial reassortant. The variant reassortant had an amino acid substitution in the hemagglutinin N244D (H3 numbering). In this report we demonstrated that the post-reassortment amino acid substitution N244D altered the antigenic specificity of HA as revealed by the loss of reactivity with an anti-H5 monoclonal antibody in hemagglutination-inhibition (HI) test. The results are discussed in association with the evolution of H5 hemagglutinin.

Authors+Show Affiliations

D.I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, Moscow, Russia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

18999893

Citation

Rudneva, I A., et al. "Post-reassortment Amino Acid Change in the Hemagglutinin of a Human-avian Influenza H5N1 Reassortant Virus Alters Its Antigenic Specificity." Acta Virologica, vol. 52, no. 3, 2008, pp. 181-4.
Rudneva IA, Timofeeva TA, Ilyushina NA, et al. Post-reassortment amino acid change in the hemagglutinin of a human-avian influenza H5N1 reassortant virus alters its antigenic specificity. Acta Virol. 2008;52(3):181-4.
Rudneva, I. A., Timofeeva, T. A., Ilyushina, N. A., Varich, N. L., Kochergin-Nikitsky, K. S., & Kaverin, N. V. (2008). Post-reassortment amino acid change in the hemagglutinin of a human-avian influenza H5N1 reassortant virus alters its antigenic specificity. Acta Virologica, 52(3), 181-4.
Rudneva IA, et al. Post-reassortment Amino Acid Change in the Hemagglutinin of a Human-avian Influenza H5N1 Reassortant Virus Alters Its Antigenic Specificity. Acta Virol. 2008;52(3):181-4. PubMed PMID: 18999893.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Post-reassortment amino acid change in the hemagglutinin of a human-avian influenza H5N1 reassortant virus alters its antigenic specificity. AU - Rudneva,I A, AU - Timofeeva,T A, AU - Ilyushina,N A, AU - Varich,N L, AU - Kochergin-Nikitsky,K S, AU - Kaverin,N V, PY - 2008/11/13/pubmed PY - 2008/12/17/medline PY - 2008/11/13/entrez SP - 181 EP - 4 JF - Acta virologica JO - Acta Virol. VL - 52 IS - 3 N2 - It was shown earlier that the reassortant influenza virus having hemagglutinin (HA) gene of A/Duck/Primorie/2621/2001 (H5N2) virus and 7 genes of A/Puerto Rico/8/34 (H1N1) virus produced low yields in embryonated chicken eggs. We found that a variant reassortant selected by serial passages in eggs produced higher yields than the initial reassortant. The variant reassortant had an amino acid substitution in the hemagglutinin N244D (H3 numbering). In this report we demonstrated that the post-reassortment amino acid substitution N244D altered the antigenic specificity of HA as revealed by the loss of reactivity with an anti-H5 monoclonal antibody in hemagglutination-inhibition (HI) test. The results are discussed in association with the evolution of H5 hemagglutinin. SN - 0001-723X UR - https://www.unboundmedicine.com/medline/citation/18999893/Post_reassortment_amino_acid_change_in_the_hemagglutinin_of_a_human_avian_influenza_H5N1_reassortant_virus_alters_its_antigenic_specificity_ L2 - http://www.aepress.sk/_downloads/dl.php?from=pubmed&journal=AV&file=2008_03_181.pdf DB - PRIME DP - Unbound Medicine ER -