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Okadaic acid protects human neuroblastoma SH-SY5Y cells from 1-methyl-4-phenylpyridinium ion-induced apoptosis.
Neurosci Lett. 2009 Jan 09; 449(2):93-7.NL

Abstract

1-methyl-4-phenylpyridinium ion (MPP(+)) has been shown to selectively inhibit mitochondrial function and induce a parkinsonism-like syndrome. MPP(+) stimulates the production of reactive oxygen species (ROS) and induces cell death in vitro. In this study, we investigated the protective effects of okadaic acid on MPP(+)-induced cell death in SH-SY5Y neuroblastoma cells. We found that MPP(+)-induced apoptosis and -ROS generation were blocked by okadaic acid. MPP(+)-mediated activation of AKT was also inhibited by okadaic acid. Taken together, these results demonstrate that okadaic acid protects against MPP(+)-induced apoptosis by blocking ROS stimulation and ROS-mediated signaling pathways in SH-SY5Y cells. These data indicated that okadaic acid could provide a therapeutic strategy for the treatment of neurodegenerative diseases including Parkinson's disease.

Authors+Show Affiliations

Department of Anatomy and Neurobiology, Biomedical Science Institute, School of Medicine, Kyung Hee University, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19000740

Citation

Ahn, Kook-Hee, et al. "Okadaic Acid Protects Human Neuroblastoma SH-SY5Y Cells From 1-methyl-4-phenylpyridinium Ion-induced Apoptosis." Neuroscience Letters, vol. 449, no. 2, 2009, pp. 93-7.
Ahn KH, Kim YS, Kim SY, et al. Okadaic acid protects human neuroblastoma SH-SY5Y cells from 1-methyl-4-phenylpyridinium ion-induced apoptosis. Neurosci Lett. 2009;449(2):93-7.
Ahn, K. H., Kim, Y. S., Kim, S. Y., Huh, Y., Park, C., & Jeong, J. W. (2009). Okadaic acid protects human neuroblastoma SH-SY5Y cells from 1-methyl-4-phenylpyridinium ion-induced apoptosis. Neuroscience Letters, 449(2), 93-7. https://doi.org/10.1016/j.neulet.2008.10.103
Ahn KH, et al. Okadaic Acid Protects Human Neuroblastoma SH-SY5Y Cells From 1-methyl-4-phenylpyridinium Ion-induced Apoptosis. Neurosci Lett. 2009 Jan 9;449(2):93-7. PubMed PMID: 19000740.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Okadaic acid protects human neuroblastoma SH-SY5Y cells from 1-methyl-4-phenylpyridinium ion-induced apoptosis. AU - Ahn,Kook-Hee, AU - Kim,Young-Sun, AU - Kim,Seon-Ye, AU - Huh,Youngbuhm, AU - Park,Chan, AU - Jeong,Joo-Won, Y1 - 2008/11/05/ PY - 2008/09/29/received PY - 2008/10/30/revised PY - 2008/10/31/accepted PY - 2008/11/13/pubmed PY - 2009/3/21/medline PY - 2008/11/13/entrez SP - 93 EP - 7 JF - Neuroscience letters JO - Neurosci Lett VL - 449 IS - 2 N2 - 1-methyl-4-phenylpyridinium ion (MPP(+)) has been shown to selectively inhibit mitochondrial function and induce a parkinsonism-like syndrome. MPP(+) stimulates the production of reactive oxygen species (ROS) and induces cell death in vitro. In this study, we investigated the protective effects of okadaic acid on MPP(+)-induced cell death in SH-SY5Y neuroblastoma cells. We found that MPP(+)-induced apoptosis and -ROS generation were blocked by okadaic acid. MPP(+)-mediated activation of AKT was also inhibited by okadaic acid. Taken together, these results demonstrate that okadaic acid protects against MPP(+)-induced apoptosis by blocking ROS stimulation and ROS-mediated signaling pathways in SH-SY5Y cells. These data indicated that okadaic acid could provide a therapeutic strategy for the treatment of neurodegenerative diseases including Parkinson's disease. SN - 0304-3940 UR - https://www.unboundmedicine.com/medline/citation/19000740/Okadaic_acid_protects_human_neuroblastoma_SH_SY5Y_cells_from_1_methyl_4_phenylpyridinium_ion_induced_apoptosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(08)01529-2 DB - PRIME DP - Unbound Medicine ER -