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[Effects of fosinopril and losartan on the expression of Toll- like receptor 4 in renal tubular epithelia cells].
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Oct; 33(10):958-65.ZN

Abstract

OBJECTIVE

To determine the mechanism of Toll-like receptor 4(TLR4) in hypertensive renal injury and the protective effect of fosinopril(Fos) and losartan(Los).

METHODS

NRK-52E was incubated into 5 groups: NRK-52E (normal control), NRK-52E+AngII, NRK-52E+AngII+Fos(10(-5) mmol/L),and NRK-52E+AngII+Los(10(-5) mmol/L), NRK-52E +AngII+Fos(10(-5) mmol/L)+Los(10(-5) mmol/L). TLR4-specific RNAi plasmids were stably transfected into NRK-52E. After 24 h, TLR4, IL-6, and TNF-alpha mRNAs were examined by reverse transcription-polymerase chain reaction(RT-PCR). TLR4 proteins were detected by Western blot, NF-kappaB nuclear translocations were tested by immunocytochemistry,and IL-6 and TNF-alpha supernatant levels were tested by enzyme linked immuno-sorbent assay(ELISA).

RESULTS

TLR4, NF-kappaB, IL-6,and TNF-alpha were highly expressed in AngII induced NRK-52E(P<0.01). In NRK-52E that was stably transfected TLR4-special RNAi plamids, TLR4 protein and mRNA expression were obviously inhibited(P<0.05). After stimulation by AngII, the TLR4, IL-6, TNF-alpha levels in the stabe transfection group were increased compared with the normal group(P<0.05). Fos or/and Los down-regulated TLR4, IL-6, and TNF-alpha expressions(P<0.05), but no cooperation was observed.

CONCLUSION

TLR4 may lead to inflammatory reaction in hypertensive renal injury. Fos or/and Los can decrease the expressions of TLR4 and correlate inflammatory factors, which may be part of the renal protective mechanism.

Authors+Show Affiliations

Department of Nephrology, Xiangya Hospital, Central South University, Changsha 410008, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

19001741

Citation

Tang, Tian-feng, et al. "[Effects of Fosinopril and Losartan On the Expression of Toll- Like Receptor 4 in Renal Tubular Epithelia Cells]." Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, vol. 33, no. 10, 2008, pp. 958-65.
Tang TF, Zhou QL, Zhu LL, et al. [Effects of fosinopril and losartan on the expression of Toll- like receptor 4 in renal tubular epithelia cells]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008;33(10):958-65.
Tang, T. F., Zhou, Q. L., Zhu, L. L., Tang, R., & Ao, X. (2008). [Effects of fosinopril and losartan on the expression of Toll- like receptor 4 in renal tubular epithelia cells]. Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, 33(10), 958-65.
Tang TF, et al. [Effects of Fosinopril and Losartan On the Expression of Toll- Like Receptor 4 in Renal Tubular Epithelia Cells]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008;33(10):958-65. PubMed PMID: 19001741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of fosinopril and losartan on the expression of Toll- like receptor 4 in renal tubular epithelia cells]. AU - Tang,Tian-feng, AU - Zhou,Qiao-ling, AU - Zhu,Li-li, AU - Tang,Rong, AU - Ao,Xiang, PY - 2008/11/13/pubmed PY - 2009/4/29/medline PY - 2008/11/13/entrez SP - 958 EP - 65 JF - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences JO - Zhong Nan Da Xue Xue Bao Yi Xue Ban VL - 33 IS - 10 N2 - OBJECTIVE: To determine the mechanism of Toll-like receptor 4(TLR4) in hypertensive renal injury and the protective effect of fosinopril(Fos) and losartan(Los). METHODS: NRK-52E was incubated into 5 groups: NRK-52E (normal control), NRK-52E+AngII, NRK-52E+AngII+Fos(10(-5) mmol/L),and NRK-52E+AngII+Los(10(-5) mmol/L), NRK-52E +AngII+Fos(10(-5) mmol/L)+Los(10(-5) mmol/L). TLR4-specific RNAi plasmids were stably transfected into NRK-52E. After 24 h, TLR4, IL-6, and TNF-alpha mRNAs were examined by reverse transcription-polymerase chain reaction(RT-PCR). TLR4 proteins were detected by Western blot, NF-kappaB nuclear translocations were tested by immunocytochemistry,and IL-6 and TNF-alpha supernatant levels were tested by enzyme linked immuno-sorbent assay(ELISA). RESULTS: TLR4, NF-kappaB, IL-6,and TNF-alpha were highly expressed in AngII induced NRK-52E(P<0.01). In NRK-52E that was stably transfected TLR4-special RNAi plamids, TLR4 protein and mRNA expression were obviously inhibited(P<0.05). After stimulation by AngII, the TLR4, IL-6, TNF-alpha levels in the stabe transfection group were increased compared with the normal group(P<0.05). Fos or/and Los down-regulated TLR4, IL-6, and TNF-alpha expressions(P<0.05), but no cooperation was observed. CONCLUSION: TLR4 may lead to inflammatory reaction in hypertensive renal injury. Fos or/and Los can decrease the expressions of TLR4 and correlate inflammatory factors, which may be part of the renal protective mechanism. SN - 1672-7347 UR - https://www.unboundmedicine.com/medline/citation/19001741/[Effects_of_fosinopril_and_losartan_on_the_expression_of_Toll__like_receptor_4_in_renal_tubular_epithelia_cells]_ L2 - http://xbyxb.csu.edu.cn/xbwk/fileup/PDF/200810958.pdf DB - PRIME DP - Unbound Medicine ER -