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[Effects of fosinopril and losartan on the expression of Toll- like receptor 4 in renal tubular epithelia cells].
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 Oct; 33(10):958-65.ZN

Abstract

OBJECTIVE

To determine the mechanism of Toll-like receptor 4(TLR4) in hypertensive renal injury and the protective effect of fosinopril(Fos) and losartan(Los).

METHODS

NRK-52E was incubated into 5 groups: NRK-52E (normal control), NRK-52E+AngII, NRK-52E+AngII+Fos(10(-5) mmol/L),and NRK-52E+AngII+Los(10(-5) mmol/L), NRK-52E +AngII+Fos(10(-5) mmol/L)+Los(10(-5) mmol/L). TLR4-specific RNAi plasmids were stably transfected into NRK-52E. After 24 h, TLR4, IL-6, and TNF-alpha mRNAs were examined by reverse transcription-polymerase chain reaction(RT-PCR). TLR4 proteins were detected by Western blot, NF-kappaB nuclear translocations were tested by immunocytochemistry,and IL-6 and TNF-alpha supernatant levels were tested by enzyme linked immuno-sorbent assay(ELISA).

RESULTS

TLR4, NF-kappaB, IL-6,and TNF-alpha were highly expressed in AngII induced NRK-52E(P<0.01). In NRK-52E that was stably transfected TLR4-special RNAi plamids, TLR4 protein and mRNA expression were obviously inhibited(P<0.05). After stimulation by AngII, the TLR4, IL-6, TNF-alpha levels in the stabe transfection group were increased compared with the normal group(P<0.05). Fos or/and Los down-regulated TLR4, IL-6, and TNF-alpha expressions(P<0.05), but no cooperation was observed.

CONCLUSION

TLR4 may lead to inflammatory reaction in hypertensive renal injury. Fos or/and Los can decrease the expressions of TLR4 and correlate inflammatory factors, which may be part of the renal protective mechanism.

Links

Publisher Full Text

Authors+Show Affiliations

Tang TF
Department of Nephrology, Xiangya Hospital, Central South University, Changsha 410008, China.
Zhou QL
No affiliation info available
Zhu LL
No affiliation info available
Tang R
No affiliation info available
Ao X
No affiliation info available

MeSH

AnimalsCell LineEpithelial CellsFosinoprilHypertensionKidney DiseasesKidney TubulesLosartanRNA InterferenceRNA, MessengerRatsToll-Like Receptor 4

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

19001741

Citation

Tang, Tian-feng, et al. "[Effects of Fosinopril and Losartan On the Expression of Toll- Like Receptor 4 in Renal Tubular Epithelia Cells]." Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, vol. 33, no. 10, 2008, pp. 958-65.
Tang TF, Zhou QL, Zhu LL, et al. [Effects of fosinopril and losartan on the expression of Toll- like receptor 4 in renal tubular epithelia cells]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008;33(10):958-65.
Tang, T. F., Zhou, Q. L., Zhu, L. L., Tang, R., & Ao, X. (2008). [Effects of fosinopril and losartan on the expression of Toll- like receptor 4 in renal tubular epithelia cells]. Zhong Nan Da Xue Xue Bao. Yi Xue Ban = Journal of Central South University. Medical Sciences, 33(10), 958-65.
Tang TF, et al. [Effects of Fosinopril and Losartan On the Expression of Toll- Like Receptor 4 in Renal Tubular Epithelia Cells]. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008;33(10):958-65. PubMed PMID: 19001741.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of fosinopril and losartan on the expression of Toll- like receptor 4 in renal tubular epithelia cells]. AU - Tang,Tian-feng, AU - Zhou,Qiao-ling, AU - Zhu,Li-li, AU - Tang,Rong, AU - Ao,Xiang, PY - 2008/11/13/pubmed PY - 2009/4/29/medline PY - 2008/11/13/entrez SP - 958 EP - 65 JF - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences JO - Zhong Nan Da Xue Xue Bao Yi Xue Ban VL - 33 IS - 10 N2 - OBJECTIVE: To determine the mechanism of Toll-like receptor 4(TLR4) in hypertensive renal injury and the protective effect of fosinopril(Fos) and losartan(Los). METHODS: NRK-52E was incubated into 5 groups: NRK-52E (normal control), NRK-52E+AngII, NRK-52E+AngII+Fos(10(-5) mmol/L),and NRK-52E+AngII+Los(10(-5) mmol/L), NRK-52E +AngII+Fos(10(-5) mmol/L)+Los(10(-5) mmol/L). TLR4-specific RNAi plasmids were stably transfected into NRK-52E. After 24 h, TLR4, IL-6, and TNF-alpha mRNAs were examined by reverse transcription-polymerase chain reaction(RT-PCR). TLR4 proteins were detected by Western blot, NF-kappaB nuclear translocations were tested by immunocytochemistry,and IL-6 and TNF-alpha supernatant levels were tested by enzyme linked immuno-sorbent assay(ELISA). RESULTS: TLR4, NF-kappaB, IL-6,and TNF-alpha were highly expressed in AngII induced NRK-52E(P<0.01). In NRK-52E that was stably transfected TLR4-special RNAi plamids, TLR4 protein and mRNA expression were obviously inhibited(P<0.05). After stimulation by AngII, the TLR4, IL-6, TNF-alpha levels in the stabe transfection group were increased compared with the normal group(P<0.05). Fos or/and Los down-regulated TLR4, IL-6, and TNF-alpha expressions(P<0.05), but no cooperation was observed. CONCLUSION: TLR4 may lead to inflammatory reaction in hypertensive renal injury. Fos or/and Los can decrease the expressions of TLR4 and correlate inflammatory factors, which may be part of the renal protective mechanism. SN - 1672-7347 UR - https://www.unboundmedicine.com/medline/citation/19001741/[Effects_of_fosinopril_and_losartan_on_the_expression_of_Toll__like_receptor_4_in_renal_tubular_epithelia_cells]_ L2 - http://xbyxb.csu.edu.cn/xbwk/fileup/PDF/200810958.pdf DB - PRIME DP - Unbound Medicine ER -