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Patients with sickle cell disease have reduced blood antioxidant protection.

Abstract

In previous studies, we found that homozygous sickle cell (HbSS) patients, compared with their healthy (HbAA) counterparts, had reduced levels of the omega-3 fatty acids, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, in red cells, platelets, and mononuclear cells. These differences were not due to lower intake of the two fatty acids. We have investigated whether reduced antioxidant status in the patients could help explain the observed phenomenon. Blood specimens previously obtained for fatty acid study from Nigerian (26 HbSS and 30 HbAA) and British (30 HbSS, 9 sickle cell-hemoglobin C/HbSC, and 15 HbAA) subjects were analyzed for antioxidant status. The Nigerian HbSS patients compared with the controls had lower plasma retinol, alpha-tocopherol, and beta-carotene concentrations (p < 0.005) and reduced activity of red cell Cu/Zn-superoxide dismutase (Cu/Zn-SOD) (p < 0.05). Similarly, the British HbSS group had reduced concentrations of plasma alpha-tocopherol (p < 0.005), and activities of red cell Cu/Zn-superoxide dismutase (p < 0.05) and Se-glutathione peroxidase (Se-GPx) (p < 0.005) than the controls. In addition, the British patients in comparison with those who had HbSC, a mild form of the disease, had lower alpha-tocopherol than that of the HbAA controls (p < 0.005). In the British sickle cell patients, there was a positive correlation between red cell ethanolamine phosphoglyceride (EPG) DHA and Cu/Zn-SOD activity (r = 0.700, p < 0.05), choline phosphoglyceride (CPG) DHA and Se-GPx activity (r = 0.605, p < 0.05), and CPG EPA and Se-GPx activity (r = 0.558, p > 0.05). Similarly, the percent DHA in red cell EPG was positively related with the activity of Se-GPx in the patients with HbSC (r = 0.674, p < 0.05). These findings suggest that the lower levels of membrane EPA and DHA in blood cells of the HbSS patients could be due to peroxidation resulting from a compromised antioxidant competence.

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  • Authors+Show Affiliations

    ,

    Institute of Brain Chemistry and Human Nutrition, London Metropolitan University, London, United Kingdom.

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    Source

    MeSH

    Adolescent
    Adult
    Aged
    Analysis of Variance
    Anemia, Sickle Cell
    Antioxidants
    Child
    Docosahexaenoic Acids
    Eicosapentaenoic Acid
    Glutathione Peroxidase
    Humans
    Middle Aged
    Nigeria
    Oxidative Stress
    Phosphatidylethanolamines
    Superoxide Dismutase
    United Kingdom
    Vitamin A
    Young Adult
    alpha-Tocopherol
    beta Carotene

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    19003736

    Citation

    Ren, Hongmei, et al. "Patients With Sickle Cell Disease Have Reduced Blood Antioxidant Protection." International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift Fur Vitamin- Und Ernahrungsforschung. Journal International De Vitaminologie Et De Nutrition, vol. 78, no. 3, 2008, pp. 139-47.
    Ren H, Ghebremeskel K, Okpala I, et al. Patients with sickle cell disease have reduced blood antioxidant protection. Int J Vitam Nutr Res. 2008;78(3):139-47.
    Ren, H., Ghebremeskel, K., Okpala, I., Lee, A., Ibegbulam, O., & Crawford, M. (2008). Patients with sickle cell disease have reduced blood antioxidant protection. International Journal for Vitamin and Nutrition Research. Internationale Zeitschrift Fur Vitamin- Und Ernahrungsforschung. Journal International De Vitaminologie Et De Nutrition, 78(3), pp. 139-47. doi:10.1024/0300-9831.78.3.139.
    Ren H, et al. Patients With Sickle Cell Disease Have Reduced Blood Antioxidant Protection. Int J Vitam Nutr Res. 2008;78(3):139-47. PubMed PMID: 19003736.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Patients with sickle cell disease have reduced blood antioxidant protection. AU - Ren,Hongmei, AU - Ghebremeskel,Kebreab, AU - Okpala,Iheanyi, AU - Lee,Ava, AU - Ibegbulam,Obike, AU - Crawford,Michael, PY - 2008/11/13/pubmed PY - 2009/2/6/medline PY - 2008/11/13/entrez SP - 139 EP - 47 JF - International journal for vitamin and nutrition research. Internationale Zeitschrift fur Vitamin- und Ernahrungsforschung. Journal international de vitaminologie et de nutrition JO - Int J Vitam Nutr Res VL - 78 IS - 3 N2 - In previous studies, we found that homozygous sickle cell (HbSS) patients, compared with their healthy (HbAA) counterparts, had reduced levels of the omega-3 fatty acids, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, in red cells, platelets, and mononuclear cells. These differences were not due to lower intake of the two fatty acids. We have investigated whether reduced antioxidant status in the patients could help explain the observed phenomenon. Blood specimens previously obtained for fatty acid study from Nigerian (26 HbSS and 30 HbAA) and British (30 HbSS, 9 sickle cell-hemoglobin C/HbSC, and 15 HbAA) subjects were analyzed for antioxidant status. The Nigerian HbSS patients compared with the controls had lower plasma retinol, alpha-tocopherol, and beta-carotene concentrations (p < 0.005) and reduced activity of red cell Cu/Zn-superoxide dismutase (Cu/Zn-SOD) (p < 0.05). Similarly, the British HbSS group had reduced concentrations of plasma alpha-tocopherol (p < 0.005), and activities of red cell Cu/Zn-superoxide dismutase (p < 0.05) and Se-glutathione peroxidase (Se-GPx) (p < 0.005) than the controls. In addition, the British patients in comparison with those who had HbSC, a mild form of the disease, had lower alpha-tocopherol than that of the HbAA controls (p < 0.005). In the British sickle cell patients, there was a positive correlation between red cell ethanolamine phosphoglyceride (EPG) DHA and Cu/Zn-SOD activity (r = 0.700, p < 0.05), choline phosphoglyceride (CPG) DHA and Se-GPx activity (r = 0.605, p < 0.05), and CPG EPA and Se-GPx activity (r = 0.558, p > 0.05). Similarly, the percent DHA in red cell EPG was positively related with the activity of Se-GPx in the patients with HbSC (r = 0.674, p < 0.05). These findings suggest that the lower levels of membrane EPA and DHA in blood cells of the HbSS patients could be due to peroxidation resulting from a compromised antioxidant competence. SN - 0300-9831 UR - https://www.unboundmedicine.com/medline/citation/19003736/Patients_with_sickle_cell_disease_have_reduced_blood_antioxidant_protection_ L2 - http://econtent.hogrefe.com/doi/full/10.1024/0300-9831.78.3.139?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -