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Characterization of thermophilic bacteria using surface-enhanced Raman scattering.
Appl Spectrosc. 2008 Nov; 62(11):1226-32.AS

Abstract

Surface-enhanced Raman scattering (SERS) can provide molecular-level information about the molecules and molecular structures in the vicinity of nanostructured noble metal surfaces such as gold and silver. The three thermophilic bacteria Bacillus licheniformis, Geobacillus stearothermophilus, and Geobacillus pallidus, a Gram-negative bacterium E. coli, and a Gram-positive bacterium B. megaterium are comparatively characterized using SERS. The SERS spectra of thermophilic bacteria are similar, while they show significant differences compared to E. coli and B. megaterium. The findings indicate that a higher number of thiol residues and possible S-S bridges are present in the cell wall structure of thermophilic bacteria, providing their stability at elevated temperatures. Incubating the thermophilic bacteria with colloidal silver suspension at longer times improved the bacteria-silver nanoparticle interaction kinetics, while increased temperature does not have a pronounced effect on spectral features. A tentative assignment of the SERS bands was attempted for thermophilic bacteria. The results indicate that SERS can be a useful tool to study bacterial cell wall molecular differences.

Authors+Show Affiliations

Yeditepe University, Faculty of Engineering and Architecture, Genetics and Bioengineering Department, Kayisdagi, 34755, Istanbul, Turkey. mculha@yeditepe.edu.trNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19007464

Citation

Culha, Mustafa, et al. "Characterization of Thermophilic Bacteria Using Surface-enhanced Raman Scattering." Applied Spectroscopy, vol. 62, no. 11, 2008, pp. 1226-32.
Culha M, Adigüzel A, Yazici MM, et al. Characterization of thermophilic bacteria using surface-enhanced Raman scattering. Appl Spectrosc. 2008;62(11):1226-32.
Culha, M., Adigüzel, A., Yazici, M. M., Kahraman, M., Sahin, F., & Güllüce, M. (2008). Characterization of thermophilic bacteria using surface-enhanced Raman scattering. Applied Spectroscopy, 62(11), 1226-32. https://doi.org/10.1366/000370208786401545
Culha M, et al. Characterization of Thermophilic Bacteria Using Surface-enhanced Raman Scattering. Appl Spectrosc. 2008;62(11):1226-32. PubMed PMID: 19007464.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of thermophilic bacteria using surface-enhanced Raman scattering. AU - Culha,Mustafa, AU - Adigüzel,Ahmet, AU - Yazici,M Müge, AU - Kahraman,Mehmet, AU - Sahin,Fikrettin, AU - Güllüce,Medine, PY - 2008/11/15/pubmed PY - 2008/12/19/medline PY - 2008/11/15/entrez SP - 1226 EP - 32 JF - Applied spectroscopy JO - Appl Spectrosc VL - 62 IS - 11 N2 - Surface-enhanced Raman scattering (SERS) can provide molecular-level information about the molecules and molecular structures in the vicinity of nanostructured noble metal surfaces such as gold and silver. The three thermophilic bacteria Bacillus licheniformis, Geobacillus stearothermophilus, and Geobacillus pallidus, a Gram-negative bacterium E. coli, and a Gram-positive bacterium B. megaterium are comparatively characterized using SERS. The SERS spectra of thermophilic bacteria are similar, while they show significant differences compared to E. coli and B. megaterium. The findings indicate that a higher number of thiol residues and possible S-S bridges are present in the cell wall structure of thermophilic bacteria, providing their stability at elevated temperatures. Incubating the thermophilic bacteria with colloidal silver suspension at longer times improved the bacteria-silver nanoparticle interaction kinetics, while increased temperature does not have a pronounced effect on spectral features. A tentative assignment of the SERS bands was attempted for thermophilic bacteria. The results indicate that SERS can be a useful tool to study bacterial cell wall molecular differences. SN - 0003-7028 UR - https://www.unboundmedicine.com/medline/citation/19007464/Characterization_of_thermophilic_bacteria_using_surface_enhanced_Raman_scattering_ L2 - https://journals.sagepub.com/doi/10.1366/000370208786401545?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -