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Improved liquid chromatography tandem mass spectrometry method for the determination of phenolic compounds in virgin olive oil.
J Chromatogr A. 2008 Dec 19; 1214(1-2):90-9.JC

Abstract

An improved liquid chromatography (LC) tandem mass spectrometry (MS/MS) method has been developed for the determination of phenolic compounds (phenyl alcohols, phenyl acids, secoiridoid derivatives, lignans and flavonoids) in virgin olive oil. The used LC technique was ultra-performance LC with columns packed with 1.7 microm particles. The obtained results (retention times, linearity, reproducibility, detection limits (LODs) and quantification limits (LOQs) for the analysis of 14 phenolic compounds in standard solutions were compared with those obtained by high-performance LC (HPLC)-fluorescence and UPLC-diode array detection (DAD). When the 1.7 microm column was used, the retention times were decreased three times with respect to conventional HPLC (5 microm). The reproducibility of these methods, expressed as relative standard deviation (RSD) in terms of concentration ranged from 0.4-5.0%. In general, the LODs and LOQs were lower in UPLC-MS/MS than the other two methodologies for all the analytes, with the exception of vanillic acid and pinoresinol which values of LODs and LOQs by HPLC-fluorescence were similar to the values obtained by UPLC-MS/MS. Afterwards, the improved UPLC-MS/MS methodology was used to determine the studied compounds in spiked refined olive oil (ROO) by combining a liquid-liquid extraction (LLE) as a sample pre-treatment technique. The recoveries of the analytes were higher than 70%, with the exception of pinoresinol and 3,4-DHPEA-EDA, which were 61% and 67%, respectively. The LODs and the LOQs ranged from 0.44-127.78 microg/kg, and from 1.11-427.78 microg/kg, respectively for all the analytes. The reproducibility of the method was lower than 3.2%. The LLE-UPLC-MS/MS was successfully applied to analyze phenolic compounds in a virgin olive oil sample within 18 min.

Authors+Show Affiliations

Food Technology Department, CeRTA-TPV, Escuela Técnica Superior de Ingeniería Agraria, Universidad de Lleida, Av/Alcalde Rovira Roure 191, 25198 Lleida, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19010478

Citation

Suárez, Manuel, et al. "Improved Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of Phenolic Compounds in Virgin Olive Oil." Journal of Chromatography. A, vol. 1214, no. 1-2, 2008, pp. 90-9.
Suárez M, Macià A, Romero MP, et al. Improved liquid chromatography tandem mass spectrometry method for the determination of phenolic compounds in virgin olive oil. J Chromatogr A. 2008;1214(1-2):90-9.
Suárez, M., Macià, A., Romero, M. P., & Motilva, M. J. (2008). Improved liquid chromatography tandem mass spectrometry method for the determination of phenolic compounds in virgin olive oil. Journal of Chromatography. A, 1214(1-2), 90-9. https://doi.org/10.1016/j.chroma.2008.10.098
Suárez M, et al. Improved Liquid Chromatography Tandem Mass Spectrometry Method for the Determination of Phenolic Compounds in Virgin Olive Oil. J Chromatogr A. 2008 Dec 19;1214(1-2):90-9. PubMed PMID: 19010478.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved liquid chromatography tandem mass spectrometry method for the determination of phenolic compounds in virgin olive oil. AU - Suárez,Manuel, AU - Macià,Alba, AU - Romero,Maria-Paz, AU - Motilva,Maria-José, Y1 - 2008/11/06/ PY - 2007/11/05/received PY - 2008/10/13/revised PY - 2008/10/15/accepted PY - 2008/11/18/pubmed PY - 2009/2/20/medline PY - 2008/11/18/entrez SP - 90 EP - 9 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1214 IS - 1-2 N2 - An improved liquid chromatography (LC) tandem mass spectrometry (MS/MS) method has been developed for the determination of phenolic compounds (phenyl alcohols, phenyl acids, secoiridoid derivatives, lignans and flavonoids) in virgin olive oil. The used LC technique was ultra-performance LC with columns packed with 1.7 microm particles. The obtained results (retention times, linearity, reproducibility, detection limits (LODs) and quantification limits (LOQs) for the analysis of 14 phenolic compounds in standard solutions were compared with those obtained by high-performance LC (HPLC)-fluorescence and UPLC-diode array detection (DAD). When the 1.7 microm column was used, the retention times were decreased three times with respect to conventional HPLC (5 microm). The reproducibility of these methods, expressed as relative standard deviation (RSD) in terms of concentration ranged from 0.4-5.0%. In general, the LODs and LOQs were lower in UPLC-MS/MS than the other two methodologies for all the analytes, with the exception of vanillic acid and pinoresinol which values of LODs and LOQs by HPLC-fluorescence were similar to the values obtained by UPLC-MS/MS. Afterwards, the improved UPLC-MS/MS methodology was used to determine the studied compounds in spiked refined olive oil (ROO) by combining a liquid-liquid extraction (LLE) as a sample pre-treatment technique. The recoveries of the analytes were higher than 70%, with the exception of pinoresinol and 3,4-DHPEA-EDA, which were 61% and 67%, respectively. The LODs and the LOQs ranged from 0.44-127.78 microg/kg, and from 1.11-427.78 microg/kg, respectively for all the analytes. The reproducibility of the method was lower than 3.2%. The LLE-UPLC-MS/MS was successfully applied to analyze phenolic compounds in a virgin olive oil sample within 18 min. SN - 0021-9673 UR - https://www.unboundmedicine.com/medline/citation/19010478/Improved_liquid_chromatography_tandem_mass_spectrometry_method_for_the_determination_of_phenolic_compounds_in_virgin_olive_oil_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)01830-X DB - PRIME DP - Unbound Medicine ER -