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Ultrasound extracted flavonoids from four varieties of Portuguese red grape skins determined by reverse-phase high-performance liquid chromatography with electrochemical detection.
Anal Chim Acta. 2008 Dec 23; 630(2):107-15.AC

Abstract

Several flavonoids present in red grape skins from four varieties of Portuguese grapes were determined by reverse-phase high-performance liquid chromatography (RP-HPLC) with electrochemical detection (ECD). Extraction of flavonoids from red grape skins was performed by ultrasonication, and hydrochloric acid in methanol was used as extraction solvent. The developed RP-HPLC method used combined isocratic and gradient elution with amperometric detection with a glassy carbon-working electrode. Good peak resolution was obtained following direct injection of a sample of red grape extract in a pH 2.20 mobile phase. Eleven different flavonoids: cyanidin-3-O-glucoside (kuromanin), delphinidin-3-O-glucoside (myrtillin), petunidin-3-O-glucoside, peonidin-3-O-glucoside, malvidin-3-O-glucoside (oenin), (+)-catechin, rutin, fisetin, myricetin, morin and quercetin, can be separated in a single run by direct injection of sample solution. The limit of detection obtained for these compounds by ECD was 20-90 pg/L, 1000 times lower when compared with photodiode array (PDA) limit of detection of 12-55 ng/L. RP-HPLC-ECD was characterized by an excellent sensitivity and selectivity, and appropriate for the simultaneous determination of these electroactive phenolic compounds present in red grape skins.

Authors+Show Affiliations

Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade de Coimbra, Coimbra, Portugal.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19012821

Citation

Novak, Ivana, et al. "Ultrasound Extracted Flavonoids From Four Varieties of Portuguese Red Grape Skins Determined By Reverse-phase High-performance Liquid Chromatography With Electrochemical Detection." Analytica Chimica Acta, vol. 630, no. 2, 2008, pp. 107-15.
Novak I, Janeiro P, Seruga M, et al. Ultrasound extracted flavonoids from four varieties of Portuguese red grape skins determined by reverse-phase high-performance liquid chromatography with electrochemical detection. Anal Chim Acta. 2008;630(2):107-15.
Novak, I., Janeiro, P., Seruga, M., & Oliveira-Brett, A. M. (2008). Ultrasound extracted flavonoids from four varieties of Portuguese red grape skins determined by reverse-phase high-performance liquid chromatography with electrochemical detection. Analytica Chimica Acta, 630(2), 107-15. https://doi.org/10.1016/j.aca.2008.10.002
Novak I, et al. Ultrasound Extracted Flavonoids From Four Varieties of Portuguese Red Grape Skins Determined By Reverse-phase High-performance Liquid Chromatography With Electrochemical Detection. Anal Chim Acta. 2008 Dec 23;630(2):107-15. PubMed PMID: 19012821.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrasound extracted flavonoids from four varieties of Portuguese red grape skins determined by reverse-phase high-performance liquid chromatography with electrochemical detection. AU - Novak,Ivana, AU - Janeiro,Patricia, AU - Seruga,Marijan, AU - Oliveira-Brett,Ana Maria, Y1 - 2008/10/14/ PY - 2008/06/12/received PY - 2008/09/30/revised PY - 2008/10/01/accepted PY - 2008/11/18/pubmed PY - 2008/12/17/medline PY - 2008/11/18/entrez SP - 107 EP - 15 JF - Analytica chimica acta JO - Anal Chim Acta VL - 630 IS - 2 N2 - Several flavonoids present in red grape skins from four varieties of Portuguese grapes were determined by reverse-phase high-performance liquid chromatography (RP-HPLC) with electrochemical detection (ECD). Extraction of flavonoids from red grape skins was performed by ultrasonication, and hydrochloric acid in methanol was used as extraction solvent. The developed RP-HPLC method used combined isocratic and gradient elution with amperometric detection with a glassy carbon-working electrode. Good peak resolution was obtained following direct injection of a sample of red grape extract in a pH 2.20 mobile phase. Eleven different flavonoids: cyanidin-3-O-glucoside (kuromanin), delphinidin-3-O-glucoside (myrtillin), petunidin-3-O-glucoside, peonidin-3-O-glucoside, malvidin-3-O-glucoside (oenin), (+)-catechin, rutin, fisetin, myricetin, morin and quercetin, can be separated in a single run by direct injection of sample solution. The limit of detection obtained for these compounds by ECD was 20-90 pg/L, 1000 times lower when compared with photodiode array (PDA) limit of detection of 12-55 ng/L. RP-HPLC-ECD was characterized by an excellent sensitivity and selectivity, and appropriate for the simultaneous determination of these electroactive phenolic compounds present in red grape skins. SN - 1873-4324 UR - https://www.unboundmedicine.com/medline/citation/19012821/Ultrasound_extracted_flavonoids_from_four_varieties_of_Portuguese_red_grape_skins_determined_by_reverse_phase_high_performance_liquid_chromatography_with_electrochemical_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2670(08)01745-5 DB - PRIME DP - Unbound Medicine ER -