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The influence of feeding linoleic, gamma-linolenic and docosahexaenoic acid rich oils on rat brain tumor fatty acids composition and fatty acid binding protein 7 mRNA expression.
Lipids Health Dis 2008; 7:45LH

Abstract

BACKGROUND

Experimental studies indicate that gamma linolenic acid (GLA) and docosahexaenoic acid (DHA) may inhibit glioma cells growth but effects of oral consumption of these fatty acids on brain tumor fatty acid composition have not been determined in vivo.

METHODS

GLA oil (GLAO; 72% GLA), DHA oil (DHAO; 73% DHA) were fed to adult wistar rats (1 mL/rat/day) starting one week prior to C6 glioma cells implantation and continued for two weeks after implantation. Control group were fed same amount of high linoleic acid safflower oil (74-77% linoleic acid). Fatty acid composition of tumor samples was determined in a set of 8-12 animals in each group and serum fatty acid in 6 animals per each group. Gene expression of tumor fatty acid binding protein 7 (FABP7), epidermal growth factor receptor (EGFR), peroxisome proliferator activated receptor gamma (PPAR-gamma) and retinoid x receptor-alpha (RXR-alpha) were determined in a set of 18 animals per group.

RESULTS

DHAO feeding increased EPA of brain tumors and decreased ratio of n-6/n-3 fatty acids. Serum levels of EPA were also increased in DHAO group. A similar trend in serum and tumor levels of DHA were observed in DHAO group but it did not achieve statistical significance. GLAO increased serum concentration of GLA but had no significant effect on tumor GLA or dihomo-gamma linolenic acid (DGLA) concentrations. Gene expression of FABP7 was up-regulated in tumors of DHAO group but no other significant effects were observed on EGFR, PPAR-gamma or RXR-alpha expression, and expression of these genes in tumors of GLAO were not different from SFO group.

CONCLUSION

Dietary supplementation of DHA containing oil could be an effective way to increase levels of long chain n-3 fatty acids in brain tumors and this increase may be mediated partly by up-regulation of FABP7 expression.

Authors+Show Affiliations

Department of Nutrition and Biochemistry, Tehran University of Medical Sciences, Tehran, Iran. jnasrolah@razi.tums.ac.irNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19014610

Citation

Nasrollahzadeh, Javad, et al. "The Influence of Feeding Linoleic, Gamma-linolenic and Docosahexaenoic Acid Rich Oils On Rat Brain Tumor Fatty Acids Composition and Fatty Acid Binding Protein 7 mRNA Expression." Lipids in Health and Disease, vol. 7, 2008, p. 45.
Nasrollahzadeh J, Siassi F, Doosti M, et al. The influence of feeding linoleic, gamma-linolenic and docosahexaenoic acid rich oils on rat brain tumor fatty acids composition and fatty acid binding protein 7 mRNA expression. Lipids Health Dis. 2008;7:45.
Nasrollahzadeh, J., Siassi, F., Doosti, M., Eshraghian, M. R., Shokri, F., Modarressi, M. H., ... Karimian, S. M. (2008). The influence of feeding linoleic, gamma-linolenic and docosahexaenoic acid rich oils on rat brain tumor fatty acids composition and fatty acid binding protein 7 mRNA expression. Lipids in Health and Disease, 7, p. 45. doi:10.1186/1476-511X-7-45.
Nasrollahzadeh J, et al. The Influence of Feeding Linoleic, Gamma-linolenic and Docosahexaenoic Acid Rich Oils On Rat Brain Tumor Fatty Acids Composition and Fatty Acid Binding Protein 7 mRNA Expression. Lipids Health Dis. 2008 Nov 16;7:45. PubMed PMID: 19014610.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The influence of feeding linoleic, gamma-linolenic and docosahexaenoic acid rich oils on rat brain tumor fatty acids composition and fatty acid binding protein 7 mRNA expression. AU - Nasrollahzadeh,Javad, AU - Siassi,Fereydoun, AU - Doosti,Mahmood, AU - Eshraghian,Mohammad Reza, AU - Shokri,Fazel, AU - Modarressi,Mohammad Hossein, AU - Mohammadi-Asl,Javad, AU - Abdi,Khosro, AU - Nikmanesh,Arash, AU - Karimian,Seyed Morteza, Y1 - 2008/11/16/ PY - 2008/08/24/received PY - 2008/11/16/accepted PY - 2008/11/19/pubmed PY - 2009/4/21/medline PY - 2008/11/19/entrez SP - 45 EP - 45 JF - Lipids in health and disease JO - Lipids Health Dis VL - 7 N2 - BACKGROUND: Experimental studies indicate that gamma linolenic acid (GLA) and docosahexaenoic acid (DHA) may inhibit glioma cells growth but effects of oral consumption of these fatty acids on brain tumor fatty acid composition have not been determined in vivo. METHODS: GLA oil (GLAO; 72% GLA), DHA oil (DHAO; 73% DHA) were fed to adult wistar rats (1 mL/rat/day) starting one week prior to C6 glioma cells implantation and continued for two weeks after implantation. Control group were fed same amount of high linoleic acid safflower oil (74-77% linoleic acid). Fatty acid composition of tumor samples was determined in a set of 8-12 animals in each group and serum fatty acid in 6 animals per each group. Gene expression of tumor fatty acid binding protein 7 (FABP7), epidermal growth factor receptor (EGFR), peroxisome proliferator activated receptor gamma (PPAR-gamma) and retinoid x receptor-alpha (RXR-alpha) were determined in a set of 18 animals per group. RESULTS: DHAO feeding increased EPA of brain tumors and decreased ratio of n-6/n-3 fatty acids. Serum levels of EPA were also increased in DHAO group. A similar trend in serum and tumor levels of DHA were observed in DHAO group but it did not achieve statistical significance. GLAO increased serum concentration of GLA but had no significant effect on tumor GLA or dihomo-gamma linolenic acid (DGLA) concentrations. Gene expression of FABP7 was up-regulated in tumors of DHAO group but no other significant effects were observed on EGFR, PPAR-gamma or RXR-alpha expression, and expression of these genes in tumors of GLAO were not different from SFO group. CONCLUSION: Dietary supplementation of DHA containing oil could be an effective way to increase levels of long chain n-3 fatty acids in brain tumors and this increase may be mediated partly by up-regulation of FABP7 expression. SN - 1476-511X UR - https://www.unboundmedicine.com/medline/citation/19014610/The_influence_of_feeding_linoleic_gamma_linolenic_and_docosahexaenoic_acid_rich_oils_on_rat_brain_tumor_fatty_acids_composition_and_fatty_acid_binding_protein_7_mRNA_expression_ L2 - https://lipidworld.biomedcentral.com/articles/10.1186/1476-511X-7-45 DB - PRIME DP - Unbound Medicine ER -