Tags

Type your tag names separated by a space and hit enter

Moricandia arvensis extracts protect against DNA damage, mutagenesis in bacteria system and scavenge the superoxide anion.
Toxicol In Vitro. 2009 Feb; 23(1):166-75.TV

Abstract

The mutagenic potential of total aqueous, total oligomers flavonoids (TOF), ethyl acetate (EA), chloroform (Chl), petroleum ether (PE) and methanol (MeOH) extracts from aerial parts of Moricandia arvensis was assessed using Ames Salmonella tester strains TA100 and TA1535 with and without metabolic activation (S9), and using plasmid pBluescript DNA assay. None of the different extracts produced a mutagenic effect, except aqueous extract when incubated with Salmonella typhimurium TA100 after metabolic activation. Likewise, the antimutagenicity of the same extracts was tested using the "Ames test". Our results showed that M. arvensis extracts possess antimutagenic effects against sodium azide (SA) in the two tested Salmonella assay systems, except metabolized aqueous and PE extracts when tested with S. typhimurium TA100 assay system. Different extracts were also found to be effective in protecting plasmid DNA against the strand breakage induced by hydroxyl radicals, except PE and aqueous extracts. Antioxidant capacity of the tested extracts was evaluated using the enzymatic (xanthine/xanthine oxidase assay) (X/XOD) and the non enzymatic (NBT/Riboflavine assay) systems. TOF extract was the more effective one in inhibiting both xanthine oxidase activity and NBT reduction.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Skandrani I
Unité de Pharmacognosie/Biologie Moléculaire 99/UR/07-03, Faculté de Pharmacie de Monastir, Rue Avicenne, Monastir 5000, Tunisia.
Bouhlel I
No affiliation info available
Limem I
No affiliation info available
Boubaker J
No affiliation info available
Bhouri W
No affiliation info available
Neffati A
No affiliation info available
Ben Sghaier M
No affiliation info available
Kilani S
No affiliation info available
Ghedira K
No affiliation info available
Ghedira-Chekir L
No affiliation info available

MeSH

AnimalsAntimutagenic AgentsAntioxidantsBrassicaceaeDNA DamageDose-Response Relationship, DrugFree Radical ScavengersMedicine, African TraditionalMutagenicity TestsMutagensPlant ExtractsRatsRibosomal Protein S9Ribosomal ProteinsSalmonella typhimuriumSodium AzideSuperoxides

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19015021

Citation

Skandrani, Ines, et al. "Moricandia Arvensis Extracts Protect Against DNA Damage, Mutagenesis in Bacteria System and Scavenge the Superoxide Anion." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 23, no. 1, 2009, pp. 166-75.
Skandrani I, Bouhlel I, Limem I, et al. Moricandia arvensis extracts protect against DNA damage, mutagenesis in bacteria system and scavenge the superoxide anion. Toxicol In Vitro. 2009;23(1):166-75.
Skandrani, I., Bouhlel, I., Limem, I., Boubaker, J., Bhouri, W., Neffati, A., Ben Sghaier, M., Kilani, S., Ghedira, K., & Ghedira-Chekir, L. (2009). Moricandia arvensis extracts protect against DNA damage, mutagenesis in bacteria system and scavenge the superoxide anion. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 23(1), 166-75. https://doi.org/10.1016/j.tiv.2008.10.010
Skandrani I, et al. Moricandia Arvensis Extracts Protect Against DNA Damage, Mutagenesis in Bacteria System and Scavenge the Superoxide Anion. Toxicol In Vitro. 2009;23(1):166-75. PubMed PMID: 19015021.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Moricandia arvensis extracts protect against DNA damage, mutagenesis in bacteria system and scavenge the superoxide anion. AU - Skandrani,Ines, AU - Bouhlel,Ines, AU - Limem,Ilef, AU - Boubaker,Jihed, AU - Bhouri,Wissem, AU - Neffati,Aicha, AU - Ben Sghaier,Mohamed, AU - Kilani,Soumaya, AU - Ghedira,Kamel, AU - Ghedira-Chekir,Leila, Y1 - 2008/10/30/ PY - 2008/07/04/received PY - 2008/10/15/revised PY - 2008/10/22/accepted PY - 2008/11/19/pubmed PY - 2009/3/25/medline PY - 2008/11/19/entrez SP - 166 EP - 75 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 23 IS - 1 N2 - The mutagenic potential of total aqueous, total oligomers flavonoids (TOF), ethyl acetate (EA), chloroform (Chl), petroleum ether (PE) and methanol (MeOH) extracts from aerial parts of Moricandia arvensis was assessed using Ames Salmonella tester strains TA100 and TA1535 with and without metabolic activation (S9), and using plasmid pBluescript DNA assay. None of the different extracts produced a mutagenic effect, except aqueous extract when incubated with Salmonella typhimurium TA100 after metabolic activation. Likewise, the antimutagenicity of the same extracts was tested using the "Ames test". Our results showed that M. arvensis extracts possess antimutagenic effects against sodium azide (SA) in the two tested Salmonella assay systems, except metabolized aqueous and PE extracts when tested with S. typhimurium TA100 assay system. Different extracts were also found to be effective in protecting plasmid DNA against the strand breakage induced by hydroxyl radicals, except PE and aqueous extracts. Antioxidant capacity of the tested extracts was evaluated using the enzymatic (xanthine/xanthine oxidase assay) (X/XOD) and the non enzymatic (NBT/Riboflavine assay) systems. TOF extract was the more effective one in inhibiting both xanthine oxidase activity and NBT reduction. SN - 0887-2333 UR - https://www.unboundmedicine.com/medline/citation/19015021/Moricandia_arvensis_extracts_protect_against_DNA_damage_mutagenesis_in_bacteria_system_and_scavenge_the_superoxide_anion_ DB - PRIME DP - Unbound Medicine ER -