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Interruption of glycerol pathway in industrial alcoholic yeasts to improve the ethanol production.
Appl Microbiol Biotechnol. 2009 Feb; 82(2):287-92.AM

Abstract

The two homologous genes GPD1 and GPD2, encoding two isoenzymes of NAD(+)-dependent glycerol-3-phosphate dehydrogenase in industrial yeast Saccharomyces cerevisiae CICIMY0086, had been deleted. The obtained two kinds of mutants gpd1Delta and gpd2Delta were studied under alcoholic fermentation conditions. gpd1Delta mutants exhibited a 4.29% (relative to the amount of substrate consumed) decrease in glycerol production and 6.83% (relative to the amount of substrate consumed) increased ethanol yield while gpd2Delta mutants exhibited a 7.95% (relative to the amount of substrate consumed) decrease in glycerol production and 7.41% (relative to the amount of substrate consumed) increased ethanol yield compared with the parental strain. The growth rate of the two mutants were slightly lower than that of the wild type under the exponential phase whereas ANG1 (gpd1Delta) and the decrease in glycerol production was not accompanied by any decline in the protein content of the strain ANG1 (gpd1Delta) but a slight decrease in the strain ANG2 (gpd2Delta). Meanwhile, dramatic decrease of acetate acid formation was observed in strain ANG1 (gpd1Delta) and ANG2 (gpd2Delta) compared to the parental strain. Therefore, it is possible to improve the ethanol yield by interruption of glycerol pathway in industrial alcoholic yeast.

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Authors+Show Affiliations

Guo ZP
The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, People's Republic of China.
Zhang L
No affiliation info available
Ding ZY
No affiliation info available
Wang ZX
No affiliation info available
Shi GY
No affiliation info available

MeSH

EthanolFermentationGlycerolGlycerol-3-Phosphate Dehydrogenase (NAD+)Industrial MicrobiologySaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Deletion

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19018525

Citation

Guo, Zhong-peng, et al. "Interruption of Glycerol Pathway in Industrial Alcoholic Yeasts to Improve the Ethanol Production." Applied Microbiology and Biotechnology, vol. 82, no. 2, 2009, pp. 287-92.
Guo ZP, Zhang L, Ding ZY, et al. Interruption of glycerol pathway in industrial alcoholic yeasts to improve the ethanol production. Appl Microbiol Biotechnol. 2009;82(2):287-92.
Guo, Z. P., Zhang, L., Ding, Z. Y., Wang, Z. X., & Shi, G. Y. (2009). Interruption of glycerol pathway in industrial alcoholic yeasts to improve the ethanol production. Applied Microbiology and Biotechnology, 82(2), 287-92. https://doi.org/10.1007/s00253-008-1777-7
Guo ZP, et al. Interruption of Glycerol Pathway in Industrial Alcoholic Yeasts to Improve the Ethanol Production. Appl Microbiol Biotechnol. 2009;82(2):287-92. PubMed PMID: 19018525.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interruption of glycerol pathway in industrial alcoholic yeasts to improve the ethanol production. AU - Guo,Zhong-peng, AU - Zhang,Liang, AU - Ding,Zhong-yang, AU - Wang,Zheng-Xiang, AU - Shi,Gui-Yang, Y1 - 2008/11/19/ PY - 2008/09/09/received PY - 2008/10/29/accepted PY - 2008/10/27/revised PY - 2008/11/20/pubmed PY - 2009/2/24/medline PY - 2008/11/20/entrez SP - 287 EP - 92 JF - Applied microbiology and biotechnology JO - Appl Microbiol Biotechnol VL - 82 IS - 2 N2 - The two homologous genes GPD1 and GPD2, encoding two isoenzymes of NAD(+)-dependent glycerol-3-phosphate dehydrogenase in industrial yeast Saccharomyces cerevisiae CICIMY0086, had been deleted. The obtained two kinds of mutants gpd1Delta and gpd2Delta were studied under alcoholic fermentation conditions. gpd1Delta mutants exhibited a 4.29% (relative to the amount of substrate consumed) decrease in glycerol production and 6.83% (relative to the amount of substrate consumed) increased ethanol yield while gpd2Delta mutants exhibited a 7.95% (relative to the amount of substrate consumed) decrease in glycerol production and 7.41% (relative to the amount of substrate consumed) increased ethanol yield compared with the parental strain. The growth rate of the two mutants were slightly lower than that of the wild type under the exponential phase whereas ANG1 (gpd1Delta) and the decrease in glycerol production was not accompanied by any decline in the protein content of the strain ANG1 (gpd1Delta) but a slight decrease in the strain ANG2 (gpd2Delta). Meanwhile, dramatic decrease of acetate acid formation was observed in strain ANG1 (gpd1Delta) and ANG2 (gpd2Delta) compared to the parental strain. Therefore, it is possible to improve the ethanol yield by interruption of glycerol pathway in industrial alcoholic yeast. SN - 1432-0614 UR - https://www.unboundmedicine.com/medline/citation/19018525/Interruption_of_glycerol_pathway_in_industrial_alcoholic_yeasts_to_improve_the_ethanol_production_ L2 - https://dx.doi.org/10.1007/s00253-008-1777-7 DB - PRIME DP - Unbound Medicine ER -