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Light responses in the mouse retina are prolonged upon targeted deletion of the HCN1 channel gene.
Eur J Neurosci. 2008 Dec; 28(11):2221-30.EJ

Abstract

Hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels contribute to pacemaker activity, and co-determine the integrative behaviour of neurons and shape their response to synaptic stimulation. Four channel isoforms, HCN1-4, have been described in mammals. Recent studies showed particularly strong expression of HCN1 channels in rods and cones of the rat retina, suggesting that HCN1 channels are involved in the shaping of light responses in both types of photoreceptors. Therefore, the loss of HCN1 channels should lead to pronounced changes in light-induced electrical responses under both scotopic and photopic conditions. This was tested using a mouse transgenic approach. We used immunohistochemistry and patch-clamp recording to study the distribution of HCN1 channels in the mouse retina. HCN1 channels were strongly expressed in rod and cone photoreceptors, as well as in some bipolar, amacrine and ganglion cell types. In electroretinograms (ERGs) from animals in which the HCN1 channel gene had been knocked out, the b-wave amplitudes were unaltered (scotopic conditions) or somewhat reduced (photopic conditions), whereas the duration of both scotopic and photopic ERG responses was strikingly prolonged. Our data suggest that in visual information processing, shortening and shaping of light responses by activation of HCN1 at the level of the photoreceptors is an important step in both scotopic and photopic pathways.

Authors+Show Affiliations

Institut für Neurowissenschaften und Biophysik, Forschungszentrum Jülich, Leo-Brandt-Strasse, D-52425 Jülich, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19019198

Citation

Knop, Gabriel C., et al. "Light Responses in the Mouse Retina Are Prolonged Upon Targeted Deletion of the HCN1 Channel Gene." The European Journal of Neuroscience, vol. 28, no. 11, 2008, pp. 2221-30.
Knop GC, Seeliger MW, Thiel F, et al. Light responses in the mouse retina are prolonged upon targeted deletion of the HCN1 channel gene. Eur J Neurosci. 2008;28(11):2221-30.
Knop, G. C., Seeliger, M. W., Thiel, F., Mataruga, A., Kaupp, U. B., Friedburg, C., Tanimoto, N., & Müller, F. (2008). Light responses in the mouse retina are prolonged upon targeted deletion of the HCN1 channel gene. The European Journal of Neuroscience, 28(11), 2221-30. https://doi.org/10.1111/j.1460-9568.2008.06512.x
Knop GC, et al. Light Responses in the Mouse Retina Are Prolonged Upon Targeted Deletion of the HCN1 Channel Gene. Eur J Neurosci. 2008;28(11):2221-30. PubMed PMID: 19019198.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Light responses in the mouse retina are prolonged upon targeted deletion of the HCN1 channel gene. AU - Knop,Gabriel C, AU - Seeliger,Mathias W, AU - Thiel,Frank, AU - Mataruga,Anja, AU - Kaupp,U Benjamin, AU - Friedburg,Christoph, AU - Tanimoto,Naoyuki, AU - Müller,Frank, Y1 - 2008/11/03/ PY - 2008/11/21/pubmed PY - 2009/2/21/medline PY - 2008/11/21/entrez SP - 2221 EP - 30 JF - The European journal of neuroscience JO - Eur J Neurosci VL - 28 IS - 11 N2 - Hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels contribute to pacemaker activity, and co-determine the integrative behaviour of neurons and shape their response to synaptic stimulation. Four channel isoforms, HCN1-4, have been described in mammals. Recent studies showed particularly strong expression of HCN1 channels in rods and cones of the rat retina, suggesting that HCN1 channels are involved in the shaping of light responses in both types of photoreceptors. Therefore, the loss of HCN1 channels should lead to pronounced changes in light-induced electrical responses under both scotopic and photopic conditions. This was tested using a mouse transgenic approach. We used immunohistochemistry and patch-clamp recording to study the distribution of HCN1 channels in the mouse retina. HCN1 channels were strongly expressed in rod and cone photoreceptors, as well as in some bipolar, amacrine and ganglion cell types. In electroretinograms (ERGs) from animals in which the HCN1 channel gene had been knocked out, the b-wave amplitudes were unaltered (scotopic conditions) or somewhat reduced (photopic conditions), whereas the duration of both scotopic and photopic ERG responses was strikingly prolonged. Our data suggest that in visual information processing, shortening and shaping of light responses by activation of HCN1 at the level of the photoreceptors is an important step in both scotopic and photopic pathways. SN - 1460-9568 UR - https://www.unboundmedicine.com/medline/citation/19019198/Light_responses_in_the_mouse_retina_are_prolonged_upon_targeted_deletion_of_the_HCN1_channel_gene_ L2 - https://doi.org/10.1111/j.1460-9568.2008.06512.x DB - PRIME DP - Unbound Medicine ER -