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Inhibitory effect of rapamycin and dexamethasone on production of IL-17 and IFN-gamma in Vogt-Koyanagi-Harada patients.
Br J Ophthalmol. 2009 Feb; 93(2):249-53.BJ

Abstract

AIMS

To evaluate the effect of rapamycin (RAPA) and dexamethasone (DEX) on the production of IL-17 and IFN-gamma by peripheral blood mononuclear cells (PBMCs) from Vogt-Koyanagi-Harada (VKH) patients and healthy individuals.

METHODS

Blood samples were drawn from 10 active VKH patients and 10 healthy individuals. PBMCs were cultured with or without anti-CD3 and anti-CD28 antibodies in the presence or absence of different concentrations of RAPA or DEX for 72 h. IL-17 and IFN-gamma concentrations in the supernatants were measured by enzyme-linked immunosorbent assay (ELISA).

RESULTS

The expression of IL-17 and IFN-gamma was significantly increased in active VKH patients compared with that in healthy controls. Both RAPA and DEX were able to significantly inhibit the production of IL-17 and IFN-gamma by PBMCs from patients and healthy controls. RAPA was able to completely inhibit IL-17 production at a dosage of 10 ng/ml but only partially suppressed IFN-gamma production even at a much higher concentration (1000 ng/ml). DEX inhibited the production of both IL-17 and IFN-gamma by approximately 70%.

CONCLUSIONS

This study indicates that both RAPA and DEX inhibit the production of IL-17 and IFN-gamma by PBMCs. RAPA is much stronger in inhibiting the production of IL-17 than DEX.

Authors+Show Affiliations

The First Clinical Hospital of ZhengZhou University, ZhengZhou, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19019941

Citation

Yang, K, et al. "Inhibitory Effect of Rapamycin and Dexamethasone On Production of IL-17 and IFN-gamma in Vogt-Koyanagi-Harada Patients." The British Journal of Ophthalmology, vol. 93, no. 2, 2009, pp. 249-53.
Yang K, Wen J, Liu X, et al. Inhibitory effect of rapamycin and dexamethasone on production of IL-17 and IFN-gamma in Vogt-Koyanagi-Harada patients. Br J Ophthalmol. 2009;93(2):249-53.
Yang, K., Wen, J., Liu, X., Kijlstra, A., Chen, L., Chi, W., Zhou, H., Huang, X., & Yang, P. (2009). Inhibitory effect of rapamycin and dexamethasone on production of IL-17 and IFN-gamma in Vogt-Koyanagi-Harada patients. The British Journal of Ophthalmology, 93(2), 249-53. https://doi.org/10.1136/bjo.2008.142489
Yang K, et al. Inhibitory Effect of Rapamycin and Dexamethasone On Production of IL-17 and IFN-gamma in Vogt-Koyanagi-Harada Patients. Br J Ophthalmol. 2009;93(2):249-53. PubMed PMID: 19019941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibitory effect of rapamycin and dexamethasone on production of IL-17 and IFN-gamma in Vogt-Koyanagi-Harada patients. AU - Yang,K, AU - Wen,J, AU - Liu,X, AU - Kijlstra,A, AU - Chen,L, AU - Chi,W, AU - Zhou,H, AU - Huang,X, AU - Yang,P, Y1 - 2008/11/19/ PY - 2008/11/21/pubmed PY - 2009/2/28/medline PY - 2008/11/21/entrez SP - 249 EP - 53 JF - The British journal of ophthalmology JO - Br J Ophthalmol VL - 93 IS - 2 N2 - AIMS: To evaluate the effect of rapamycin (RAPA) and dexamethasone (DEX) on the production of IL-17 and IFN-gamma by peripheral blood mononuclear cells (PBMCs) from Vogt-Koyanagi-Harada (VKH) patients and healthy individuals. METHODS: Blood samples were drawn from 10 active VKH patients and 10 healthy individuals. PBMCs were cultured with or without anti-CD3 and anti-CD28 antibodies in the presence or absence of different concentrations of RAPA or DEX for 72 h. IL-17 and IFN-gamma concentrations in the supernatants were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: The expression of IL-17 and IFN-gamma was significantly increased in active VKH patients compared with that in healthy controls. Both RAPA and DEX were able to significantly inhibit the production of IL-17 and IFN-gamma by PBMCs from patients and healthy controls. RAPA was able to completely inhibit IL-17 production at a dosage of 10 ng/ml but only partially suppressed IFN-gamma production even at a much higher concentration (1000 ng/ml). DEX inhibited the production of both IL-17 and IFN-gamma by approximately 70%. CONCLUSIONS: This study indicates that both RAPA and DEX inhibit the production of IL-17 and IFN-gamma by PBMCs. RAPA is much stronger in inhibiting the production of IL-17 than DEX. SN - 1468-2079 UR - https://www.unboundmedicine.com/medline/citation/19019941/Inhibitory_effect_of_rapamycin_and_dexamethasone_on_production_of_IL_17_and_IFN_gamma_in_Vogt_Koyanagi_Harada_patients_ L2 - http://bjo.bmj.com/cgi/pmidlookup?view=long&pmid=19019941 DB - PRIME DP - Unbound Medicine ER -