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Characterization of T. aestivum-H. californicum chromosome addition lines DA2H and MA5H.
J Genet Genomics. 2008 Nov; 35(11):673-8.JG

Abstract

In order to transfer useful genes of Hordeum californicum into common wheat (Triticum aestivum L.), the T. aestivum c.v. Chinese Spring (CS)-H. californicum amphiploid was crossed to CS, and its backcrossing and self-fertilized progenies were analyzed by morphological observation, cytological, biochemical and molecular marker techniques. Alien addition lines with two H. californicum chromosomes were identified and their genetic constitution was characterized. STS-PCR analysis using chromosome 2B specific markers indicated that chromosome H3 of H. californicum belongs to homologous group 2, and was thus designated 2H. SDS-PAGE showed that chromosome H2 of H. californicum belongs to homologous group 5, and was designated 5H. The CS-H. californicum amphiploid and the chromosome addition lines (DA2H and MA5H) identified were evaluated for powdery mildew (Erysiphe graminis f. sp. triticii) resistance in field. The preliminary results indicated that the amphiploid showed higher powdery mildew resistance than CS. However, chromosome addition lines DA2H and MA5H were highly susceptible to powdery mildew, indicating that major powdery mildew resistant genes of H. californicum should be located on chromosomes other than 2H and 5H.

Authors+Show Affiliations

State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University, Nanjing 210095, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19022201

Citation

Kong, Fang, et al. "Characterization of T. aestivum-H. Californicum Chromosome Addition Lines DA2H and MA5H." Journal of Genetics and Genomics = Yi Chuan Xue Bao, vol. 35, no. 11, 2008, pp. 673-8.
Kong F, Wang H, Cao A, et al. Characterization of T. aestivum-H. californicum chromosome addition lines DA2H and MA5H. J Genet Genomics. 2008;35(11):673-8.
Kong, F., Wang, H., Cao, A., Qin, B., Ji, J., Wang, S., & Wang, X. E. (2008). Characterization of T. aestivum-H. californicum chromosome addition lines DA2H and MA5H. Journal of Genetics and Genomics = Yi Chuan Xue Bao, 35(11), 673-8. https://doi.org/10.1016/S1673-8527(08)60089-2
Kong F, et al. Characterization of T. aestivum-H. Californicum Chromosome Addition Lines DA2H and MA5H. J Genet Genomics. 2008;35(11):673-8. PubMed PMID: 19022201.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of T. aestivum-H. californicum chromosome addition lines DA2H and MA5H. AU - Kong,Fang, AU - Wang,Haiyan, AU - Cao,Aizhong, AU - Qin,Bi, AU - Ji,Jianhui, AU - Wang,Suling, AU - Wang,Xiu-E, PY - 2008/05/12/received PY - 2008/08/01/revised PY - 2008/08/18/accepted PY - 2008/11/22/pubmed PY - 2009/3/31/medline PY - 2008/11/22/entrez SP - 673 EP - 8 JF - Journal of genetics and genomics = Yi chuan xue bao JO - J Genet Genomics VL - 35 IS - 11 N2 - In order to transfer useful genes of Hordeum californicum into common wheat (Triticum aestivum L.), the T. aestivum c.v. Chinese Spring (CS)-H. californicum amphiploid was crossed to CS, and its backcrossing and self-fertilized progenies were analyzed by morphological observation, cytological, biochemical and molecular marker techniques. Alien addition lines with two H. californicum chromosomes were identified and their genetic constitution was characterized. STS-PCR analysis using chromosome 2B specific markers indicated that chromosome H3 of H. californicum belongs to homologous group 2, and was thus designated 2H. SDS-PAGE showed that chromosome H2 of H. californicum belongs to homologous group 5, and was designated 5H. The CS-H. californicum amphiploid and the chromosome addition lines (DA2H and MA5H) identified were evaluated for powdery mildew (Erysiphe graminis f. sp. triticii) resistance in field. The preliminary results indicated that the amphiploid showed higher powdery mildew resistance than CS. However, chromosome addition lines DA2H and MA5H were highly susceptible to powdery mildew, indicating that major powdery mildew resistant genes of H. californicum should be located on chromosomes other than 2H and 5H. SN - 1673-8527 UR - https://www.unboundmedicine.com/medline/citation/19022201/Characterization_of_T__aestivum_H__californicum_chromosome_addition_lines_DA2H_and_MA5H_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1673-8527(08)60089-2 DB - PRIME DP - Unbound Medicine ER -