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Performance of a commercial rapid dengue NS1 antigen immunochromatography test with reference to dengue NS1 antigen-capture ELISA.
J Virol Methods. 2009 Feb; 155(2):157-60.JV

Abstract

The performance of a commercial immunochromatography test for rapid detection of dengue NS1 antigen present in serum or plasma of patients was evaluated against a commercial dengue NS1 antigen-capture ELISA. The rapid immunochromatography test gave an overall sensitivity of 90.4% with a specificity of 99.5%. The sensitivity was highest for serum samples from which virus was isolated (96.3%) and lowest for those from which virus was not isolated and RT-PCR was negative (76.4%). The sensitivity was significantly higher for serum samples from patients with acute primary dengue (92.3%) than those from patients with acute secondary dengue (79.1%). The positive predictive value and negative predictive value of this commercial immunochromatography test were 99.6% and 87.9% respectively.

Authors+Show Affiliations

Virology Unit, Infectious Disease Research Centre, Institute for Medical Research, Jalan Pahang, 50588 Kuala Lumpur, Malaysia. zainah@imr.gov.myNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

19022293

Citation

Zainah, S, et al. "Performance of a Commercial Rapid Dengue NS1 Antigen Immunochromatography Test With Reference to Dengue NS1 Antigen-capture ELISA." Journal of Virological Methods, vol. 155, no. 2, 2009, pp. 157-60.
Zainah S, Wahab AH, Mariam M, et al. Performance of a commercial rapid dengue NS1 antigen immunochromatography test with reference to dengue NS1 antigen-capture ELISA. J Virol Methods. 2009;155(2):157-60.
Zainah, S., Wahab, A. H., Mariam, M., Fauziah, M. K., Khairul, A. H., Roslina, I., Sairulakhma, A., Kadimon, S. S., Jais, M. S., & Chua, K. B. (2009). Performance of a commercial rapid dengue NS1 antigen immunochromatography test with reference to dengue NS1 antigen-capture ELISA. Journal of Virological Methods, 155(2), 157-60. https://doi.org/10.1016/j.jviromet.2008.10.016
Zainah S, et al. Performance of a Commercial Rapid Dengue NS1 Antigen Immunochromatography Test With Reference to Dengue NS1 Antigen-capture ELISA. J Virol Methods. 2009;155(2):157-60. PubMed PMID: 19022293.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Performance of a commercial rapid dengue NS1 antigen immunochromatography test with reference to dengue NS1 antigen-capture ELISA. AU - Zainah,S, AU - Wahab,A H Abdul, AU - Mariam,M, AU - Fauziah,M K, AU - Khairul,A H, AU - Roslina,I, AU - Sairulakhma,A, AU - Kadimon,S S, AU - Jais,M S Mat, AU - Chua,K B, Y1 - 2008/12/02/ PY - 2008/07/21/received PY - 2008/10/15/revised PY - 2008/10/20/accepted PY - 2008/11/22/pubmed PY - 2009/3/19/medline PY - 2008/11/22/entrez SP - 157 EP - 60 JF - Journal of virological methods JO - J Virol Methods VL - 155 IS - 2 N2 - The performance of a commercial immunochromatography test for rapid detection of dengue NS1 antigen present in serum or plasma of patients was evaluated against a commercial dengue NS1 antigen-capture ELISA. The rapid immunochromatography test gave an overall sensitivity of 90.4% with a specificity of 99.5%. The sensitivity was highest for serum samples from which virus was isolated (96.3%) and lowest for those from which virus was not isolated and RT-PCR was negative (76.4%). The sensitivity was significantly higher for serum samples from patients with acute primary dengue (92.3%) than those from patients with acute secondary dengue (79.1%). The positive predictive value and negative predictive value of this commercial immunochromatography test were 99.6% and 87.9% respectively. SN - 0166-0934 UR - https://www.unboundmedicine.com/medline/citation/19022293/Performance_of_a_commercial_rapid_dengue_NS1_antigen_immunochromatography_test_with_reference_to_dengue_NS1_antigen_capture_ELISA_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-0934(08)00387-X DB - PRIME DP - Unbound Medicine ER -