Tags

Type your tag names separated by a space and hit enter

Involvement of ubiquitin proteasome system in protective mechanisms of Puerarin to MPP(+)-elicited apoptosis.
Neurosci Res. 2009 Jan; 63(1):52-8.NR

Abstract

It has been well documented that dysfunction of ubiquitin proteasome system (UPS) in the neuron exacerbated the Parkinson's disease (PD). However, whether or not UPS is involved in the protective effect of Puerarin on 1-Methyl-4-Phenyl-1, 2, 3, 6-Tetrahydropyridine (MPP(+))-elicited cell death is yet to be elucidated. In this study, treatment of SH-SY5Y cells with 1mM MPP(+)-elicited a characteristic apoptotic cell death and pretreatment with Puerarin protected cells against MPP(+)-induced apoptosis as evidenced by promoting cell viability, improving morphological changes and reducing apoptotic rate. To further explore the potential protective mechanism of Puerarin in MPP(+)-induced SH-SY5Y cell death, UPS activity, mitochondria-dependent apoptosis and caspase-3 activity were measured. Puerarin pretreatment attenuated MPP(+)-induced dysfunction of protease activity, thereby reducing accumulation of ubiquitin-conjugated proteins. Meanwhile, caspase-3 activity was remarkably attenuated by Puerarin. In addition, the ratio of bcl-2/bax was increased by Puerarin in comparison with MPP(+)-treated group. Taken together, these results suggest that Puerarin could protect MPP(+)-induced SH-SY5Y cells from apoptosis by regulating the function of UPS.

Authors+Show Affiliations

Dept of Pharmacology, Anhui Medical University, Hefei 230032, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19022306

Citation

Cheng, Yue-Fa, et al. "Involvement of Ubiquitin Proteasome System in Protective Mechanisms of Puerarin to MPP(+)-elicited Apoptosis." Neuroscience Research, vol. 63, no. 1, 2009, pp. 52-8.
Cheng YF, Zhu GQ, Wang M, et al. Involvement of ubiquitin proteasome system in protective mechanisms of Puerarin to MPP(+)-elicited apoptosis. Neurosci Res. 2009;63(1):52-8.
Cheng, Y. F., Zhu, G. Q., Wang, M., Cheng, H., Zhou, A., Wang, N., Fang, N., Wang, X. C., Xiao, X. Q., Chen, Z. W., & Li, Q. L. (2009). Involvement of ubiquitin proteasome system in protective mechanisms of Puerarin to MPP(+)-elicited apoptosis. Neuroscience Research, 63(1), 52-8. https://doi.org/10.1016/j.neures.2008.10.009
Cheng YF, et al. Involvement of Ubiquitin Proteasome System in Protective Mechanisms of Puerarin to MPP(+)-elicited Apoptosis. Neurosci Res. 2009;63(1):52-8. PubMed PMID: 19022306.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of ubiquitin proteasome system in protective mechanisms of Puerarin to MPP(+)-elicited apoptosis. AU - Cheng,Yue-Fa, AU - Zhu,Guo-Qi, AU - Wang,Mei, AU - Cheng,Hui, AU - Zhou,An, AU - Wang,Ning, AU - Fang,Nianbai, AU - Wang,Xun-Cui, AU - Xiao,Xiao-Qiu, AU - Chen,Zhi-Wu, AU - Li,Qing-Lin, Y1 - 2008/10/30/ PY - 2008/08/27/received PY - 2008/10/07/revised PY - 2008/10/14/accepted PY - 2008/11/22/pubmed PY - 2009/4/21/medline PY - 2008/11/22/entrez SP - 52 EP - 8 JF - Neuroscience research JO - Neurosci Res VL - 63 IS - 1 N2 - It has been well documented that dysfunction of ubiquitin proteasome system (UPS) in the neuron exacerbated the Parkinson's disease (PD). However, whether or not UPS is involved in the protective effect of Puerarin on 1-Methyl-4-Phenyl-1, 2, 3, 6-Tetrahydropyridine (MPP(+))-elicited cell death is yet to be elucidated. In this study, treatment of SH-SY5Y cells with 1mM MPP(+)-elicited a characteristic apoptotic cell death and pretreatment with Puerarin protected cells against MPP(+)-induced apoptosis as evidenced by promoting cell viability, improving morphological changes and reducing apoptotic rate. To further explore the potential protective mechanism of Puerarin in MPP(+)-induced SH-SY5Y cell death, UPS activity, mitochondria-dependent apoptosis and caspase-3 activity were measured. Puerarin pretreatment attenuated MPP(+)-induced dysfunction of protease activity, thereby reducing accumulation of ubiquitin-conjugated proteins. Meanwhile, caspase-3 activity was remarkably attenuated by Puerarin. In addition, the ratio of bcl-2/bax was increased by Puerarin in comparison with MPP(+)-treated group. Taken together, these results suggest that Puerarin could protect MPP(+)-induced SH-SY5Y cells from apoptosis by regulating the function of UPS. SN - 0168-0102 UR - https://www.unboundmedicine.com/medline/citation/19022306/Involvement_of_ubiquitin_proteasome_system_in_protective_mechanisms_of_Puerarin_to_MPP_+__elicited_apoptosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-0102(08)00269-1 DB - PRIME DP - Unbound Medicine ER -