Tags

Type your tag names separated by a space and hit enter

Genotoxicity of the herbicide formulation Roundup (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test.
Mutat Res. 2009 Jan 31; 672(2):95-102.MR

Abstract

The genotoxicity of pesticides is an issue of worldwide concern. The present study was undertaken to evaluate the genotoxic potential of a widely used herbicide formulation, Roundup (glyphosate), in erythrocytes of broad-snouted caiman (Caiman latirostris) after in ovo exposure. Caiman embryos were exposed at early embryonic stage to different sub-lethal concentrations of Roundup (50, 100, 200, 300, 400, 500, 750, 1000, 1250 and 1750microg/egg). At time of hatching, blood samples were obtained from each animal and two short-term tests, the Comet assay and the Micronucleus (MN) test, were performed on erythrocytes to assess DNA damage. A significant increase in DNA damage was observed at a concentration of 500microg/egg or higher, compared to untreated control animals (p<0.05). Results from both the Comet assay and the MN test revealed a concentration-dependent effect. This study demonstrated adverse effects of Roundup on DNA of C. latirostris and confirmed that the Comet assay and the MN test applied on caiman erythrocytes are useful tools in determining potential genotoxicity of pesticides. The identification of sentinel species as well as sensitive biomarkers among the natural biota is imperative to thoroughly evaluate genetic damage, which has significant consequences for short- and long-term survival of the natural species.

Authors+Show Affiliations

Proyecto Yacaré (Gobierno Santa Fe/MUPCN), A. del Valle 8700, CP: 3000, Santa Fe, Argentina. gisepoletta@fbcb.unl.edu.arNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19022394

Citation

Poletta, G L., et al. "Genotoxicity of the Herbicide Formulation Roundup (glyphosate) in Broad-snouted Caiman (Caiman Latirostris) Evidenced By the Comet Assay and the Micronucleus Test." Mutation Research, vol. 672, no. 2, 2009, pp. 95-102.
Poletta GL, Larriera A, Kleinsorge E, et al. Genotoxicity of the herbicide formulation Roundup (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test. Mutat Res. 2009;672(2):95-102.
Poletta, G. L., Larriera, A., Kleinsorge, E., & Mudry, M. D. (2009). Genotoxicity of the herbicide formulation Roundup (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test. Mutation Research, 672(2), 95-102. https://doi.org/10.1016/j.mrgentox.2008.10.007
Poletta GL, et al. Genotoxicity of the Herbicide Formulation Roundup (glyphosate) in Broad-snouted Caiman (Caiman Latirostris) Evidenced By the Comet Assay and the Micronucleus Test. Mutat Res. 2009 Jan 31;672(2):95-102. PubMed PMID: 19022394.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxicity of the herbicide formulation Roundup (glyphosate) in broad-snouted caiman (Caiman latirostris) evidenced by the Comet assay and the Micronucleus test. AU - Poletta,G L, AU - Larriera,A, AU - Kleinsorge,E, AU - Mudry,M D, Y1 - 2008/10/30/ PY - 2008/08/06/received PY - 2008/10/06/revised PY - 2008/10/08/accepted PY - 2008/11/22/entrez PY - 2008/11/22/pubmed PY - 2009/4/1/medline SP - 95 EP - 102 JF - Mutation research JO - Mutat Res VL - 672 IS - 2 N2 - The genotoxicity of pesticides is an issue of worldwide concern. The present study was undertaken to evaluate the genotoxic potential of a widely used herbicide formulation, Roundup (glyphosate), in erythrocytes of broad-snouted caiman (Caiman latirostris) after in ovo exposure. Caiman embryos were exposed at early embryonic stage to different sub-lethal concentrations of Roundup (50, 100, 200, 300, 400, 500, 750, 1000, 1250 and 1750microg/egg). At time of hatching, blood samples were obtained from each animal and two short-term tests, the Comet assay and the Micronucleus (MN) test, were performed on erythrocytes to assess DNA damage. A significant increase in DNA damage was observed at a concentration of 500microg/egg or higher, compared to untreated control animals (p<0.05). Results from both the Comet assay and the MN test revealed a concentration-dependent effect. This study demonstrated adverse effects of Roundup on DNA of C. latirostris and confirmed that the Comet assay and the MN test applied on caiman erythrocytes are useful tools in determining potential genotoxicity of pesticides. The identification of sentinel species as well as sensitive biomarkers among the natural biota is imperative to thoroughly evaluate genetic damage, which has significant consequences for short- and long-term survival of the natural species. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/19022394/Genotoxicity_of_the_herbicide_formulation_Roundup__glyphosate__in_broad_snouted_caiman__Caiman_latirostris__evidenced_by_the_Comet_assay_and_the_Micronucleus_test_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(08)00301-X DB - PRIME DP - Unbound Medicine ER -