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Characterization and expression analysis of a gene encoding a secreted lipase-like protein expressed in the salivary glands of the larval Hessian fly, Mayetiola destructor (Say).
J Insect Physiol. 2009 Feb; 55(2):104-11.JI

Abstract

In a salivary gland transcriptomics study we identified a cDNA with a full-length open reading frame for a gene (MdesL1) encoding a lipase-like protein expressed in the salivary glands of the larval Hessian fly, Mayetiola destructor (Say). Fluorescent in situ hybridization on salivary polytenes positioned MdesL1 on the long arm of Autosome 1. BLASTp and conserved domain searches revealed the deduced amino acid sequence contained a lipase superfamily domain with similarity to lipases and phospholipases from other insects. A secretion signal peptide was identified at the amino terminus of the deduced amino acid sequence. Analysis of the transcript of MdesL1 in larval Hessian fly tissues by quantitative real-time PCR (qPCR) revealed the greatest abundance was in salivary glands. Analysis of transcript levels during development showed the greatest level was detected in feeding 1st-instar and early 2nd-instar larvae. Transcript levels increased dramatically over time in larvae feeding on susceptible wheat but were detected at low levels in larvae feeding on resistant wheat. These data suggest the protein encoded by MdesL1 is likely secreted into host-plant cells during larval feeding and could be involved in extra-oral digestion and changes in host-cell permeability or in generating a second messenger in a host-cell-signaling cascade.

Authors+Show Affiliations

Crop Production and Pest Control Research Unit, USDA-ARS, Department of Entomology, Purdue University, West Lafayette, IN 47907, USA. shukle@purdue.eduNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19026654

Citation

Shukle, Richard H., et al. "Characterization and Expression Analysis of a Gene Encoding a Secreted Lipase-like Protein Expressed in the Salivary Glands of the Larval Hessian Fly, Mayetiola Destructor (Say)." Journal of Insect Physiology, vol. 55, no. 2, 2009, pp. 104-11.
Shukle RH, Mittapalli O, Morton PK, et al. Characterization and expression analysis of a gene encoding a secreted lipase-like protein expressed in the salivary glands of the larval Hessian fly, Mayetiola destructor (Say). J Insect Physiol. 2009;55(2):104-11.
Shukle, R. H., Mittapalli, O., Morton, P. K., & Chen, M. S. (2009). Characterization and expression analysis of a gene encoding a secreted lipase-like protein expressed in the salivary glands of the larval Hessian fly, Mayetiola destructor (Say). Journal of Insect Physiology, 55(2), 104-11. https://doi.org/10.1016/j.jinsphys.2008.10.008
Shukle RH, et al. Characterization and Expression Analysis of a Gene Encoding a Secreted Lipase-like Protein Expressed in the Salivary Glands of the Larval Hessian Fly, Mayetiola Destructor (Say). J Insect Physiol. 2009;55(2):104-11. PubMed PMID: 19026654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization and expression analysis of a gene encoding a secreted lipase-like protein expressed in the salivary glands of the larval Hessian fly, Mayetiola destructor (Say). AU - Shukle,Richard H, AU - Mittapalli,Omprakash, AU - Morton,Philip K, AU - Chen,Ming-Shun, Y1 - 2008/12/18/ PY - 2008/07/18/received PY - 2008/10/17/revised PY - 2008/10/20/accepted PY - 2008/11/26/pubmed PY - 2009/4/28/medline PY - 2008/11/26/entrez SP - 104 EP - 11 JF - Journal of insect physiology JO - J Insect Physiol VL - 55 IS - 2 N2 - In a salivary gland transcriptomics study we identified a cDNA with a full-length open reading frame for a gene (MdesL1) encoding a lipase-like protein expressed in the salivary glands of the larval Hessian fly, Mayetiola destructor (Say). Fluorescent in situ hybridization on salivary polytenes positioned MdesL1 on the long arm of Autosome 1. BLASTp and conserved domain searches revealed the deduced amino acid sequence contained a lipase superfamily domain with similarity to lipases and phospholipases from other insects. A secretion signal peptide was identified at the amino terminus of the deduced amino acid sequence. Analysis of the transcript of MdesL1 in larval Hessian fly tissues by quantitative real-time PCR (qPCR) revealed the greatest abundance was in salivary glands. Analysis of transcript levels during development showed the greatest level was detected in feeding 1st-instar and early 2nd-instar larvae. Transcript levels increased dramatically over time in larvae feeding on susceptible wheat but were detected at low levels in larvae feeding on resistant wheat. These data suggest the protein encoded by MdesL1 is likely secreted into host-plant cells during larval feeding and could be involved in extra-oral digestion and changes in host-cell permeability or in generating a second messenger in a host-cell-signaling cascade. SN - 0022-1910 UR - https://www.unboundmedicine.com/medline/citation/19026654/Characterization_and_expression_analysis_of_a_gene_encoding_a_secreted_lipase_like_protein_expressed_in_the_salivary_glands_of_the_larval_Hessian_fly_Mayetiola_destructor__Say__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-1910(08)00219-9 DB - PRIME DP - Unbound Medicine ER -