Tags

Type your tag names separated by a space and hit enter

A rapid and sensitive liquid chromatography-tandem mass spectrometric method for the determination of timosaponin B-II in blood plasma and a study of the pharmacokinetics of saponin in the rat.
J Pharm Biomed Anal. 2008 Dec 15; 48(5):1411-6.JP

Abstract

A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of Timosaponin B-II (TB-II), a pharmacologically active constituent isolated from Anemarrhena asphodeloides. This method was used to examine the pharmacokinetics and bioavailability of TB-II in rats using ginsenoside Re as an internal standard. After simple protein precipitation of the plasma samples with acetonitrile, the analytes were separated on an ODS column (150 mm x 2.1 mm i.d., 5 microm) with the mobile phase of acetonitrile-water (35:65, v/v) containing 0.05% formic acid and detected by electrospray ionization mass spectrometry in the negative multiple reaction monitoring (MRM) mode with a chromatographic run time of 3.0 min. The calibration curves were linear over the range of 5-15,000 ng/ml and the lower limit of quantification (LLOQ) was 5 ng/ml in rat plasma. In this range, relative standard deviations (R.S.D.) were <7.4% for intra-day precision and <9.0% for inter-day precision. The accuracy was within the range of 97.7-107.3%. The method was successfully applied to assess the pharmacokinetics and oral bioavailability of TB-II after intravenous and oral administration in rats, with the oral bioavailability being only 1.1%.

Authors+Show Affiliations

Department of Pharmacy, Changzheng Hospital, Second Military Medical University, No. 415 Fengyang Road, Shanghai 200003, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19027255

Citation

Cai, Fei, et al. "A Rapid and Sensitive Liquid Chromatography-tandem Mass Spectrometric Method for the Determination of Timosaponin B-II in Blood Plasma and a Study of the Pharmacokinetics of Saponin in the Rat." Journal of Pharmaceutical and Biomedical Analysis, vol. 48, no. 5, 2008, pp. 1411-6.
Cai F, Sun L, Gao S, et al. A rapid and sensitive liquid chromatography-tandem mass spectrometric method for the determination of timosaponin B-II in blood plasma and a study of the pharmacokinetics of saponin in the rat. J Pharm Biomed Anal. 2008;48(5):1411-6.
Cai, F., Sun, L., Gao, S., Yang, Y., Yang, Q., & Chen, W. (2008). A rapid and sensitive liquid chromatography-tandem mass spectrometric method for the determination of timosaponin B-II in blood plasma and a study of the pharmacokinetics of saponin in the rat. Journal of Pharmaceutical and Biomedical Analysis, 48(5), 1411-6. https://doi.org/10.1016/j.jpba.2008.09.032
Cai F, et al. A Rapid and Sensitive Liquid Chromatography-tandem Mass Spectrometric Method for the Determination of Timosaponin B-II in Blood Plasma and a Study of the Pharmacokinetics of Saponin in the Rat. J Pharm Biomed Anal. 2008 Dec 15;48(5):1411-6. PubMed PMID: 19027255.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A rapid and sensitive liquid chromatography-tandem mass spectrometric method for the determination of timosaponin B-II in blood plasma and a study of the pharmacokinetics of saponin in the rat. AU - Cai,Fei, AU - Sun,Lianna, AU - Gao,Shouhong, AU - Yang,Yang, AU - Yang,Qi, AU - Chen,Wansheng, Y1 - 2008/09/30/ PY - 2008/07/27/received PY - 2008/09/17/revised PY - 2008/09/18/accepted PY - 2008/11/26/pubmed PY - 2009/4/25/medline PY - 2008/11/26/entrez SP - 1411 EP - 6 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 48 IS - 5 N2 - A rapid, sensitive and selective liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed and validated for the determination of Timosaponin B-II (TB-II), a pharmacologically active constituent isolated from Anemarrhena asphodeloides. This method was used to examine the pharmacokinetics and bioavailability of TB-II in rats using ginsenoside Re as an internal standard. After simple protein precipitation of the plasma samples with acetonitrile, the analytes were separated on an ODS column (150 mm x 2.1 mm i.d., 5 microm) with the mobile phase of acetonitrile-water (35:65, v/v) containing 0.05% formic acid and detected by electrospray ionization mass spectrometry in the negative multiple reaction monitoring (MRM) mode with a chromatographic run time of 3.0 min. The calibration curves were linear over the range of 5-15,000 ng/ml and the lower limit of quantification (LLOQ) was 5 ng/ml in rat plasma. In this range, relative standard deviations (R.S.D.) were <7.4% for intra-day precision and <9.0% for inter-day precision. The accuracy was within the range of 97.7-107.3%. The method was successfully applied to assess the pharmacokinetics and oral bioavailability of TB-II after intravenous and oral administration in rats, with the oral bioavailability being only 1.1%. SN - 0731-7085 UR - https://www.unboundmedicine.com/medline/citation/19027255/A_rapid_and_sensitive_liquid_chromatography_tandem_mass_spectrometric_method_for_the_determination_of_timosaponin_B_II_in_blood_plasma_and_a_study_of_the_pharmacokinetics_of_saponin_in_the_rat_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(08)00515-3 DB - PRIME DP - Unbound Medicine ER -