TY - JOUR T1 - Capillary electrokinetic chromatography of insulin and related synthetic analogues. AU - Ortner,K, AU - Buchberger,W, AU - Himmelsbach,M, Y1 - 2008/11/07/ PY - 2008/07/03/received PY - 2008/10/26/revised PY - 2008/11/03/accepted PY - 2008/11/26/pubmed PY - 2009/5/12/medline PY - 2008/11/26/entrez SP - 2953 EP - 7 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 14 N2 - With the implementation of recombinant DNA technology in the pharmaceutical industry, some synthetic insulins have been developed in order to improve the therapy of diabetes. These analogues differ only slightly in the amino acid sequence, therefore displaying a great challenge for analytical chemistry. Within the work presented in this paper, capillary zone electrophoresis (CZE), micellar electrokinetic chromatography (MEKC) with sodium dodecylsulphate (SDS) as micelle-forming agent, and microemulsion electrokinetic chromatography (MEEKC) with microemulsions consisting of SDS, n-octane and 1-butanol were investigated for the separation of human insulin and five synthetic analogues. Best results were achieved with a solvent-modified MEKC system consisting of 100mM sodium dodecyl sulphate and 15% acetonitrile in 10mM borate buffer (pH 9.2). A similar system based on perfluorooctanoic acid as micelle-forming agent in ammonium acetate (pH 9.2) was successfully employed for the hyphenation with a quadrupole/time-of-flight mass spectrometer via a sheath-flow interface. In this case, detection limits at 10mg/L could be achieved. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19027906/Capillary_electrokinetic_chromatography_of_insulin_and_related_synthetic_analogues_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(08)01940-7 DB - PRIME DP - Unbound Medicine ER -